转化生长因子对巨噬细胞抗新生隐球菌功能的影响

中国真菌学杂志　 2017, Vol. 12 　Issue (2): 65-68.

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转化生长因子对巨噬细胞抗新生隐球菌功能的影响

李颖芳, 桑军军, 李娟, 姜伟伟, 廖万清, 潘炜华

上海长征医院皮肤病与真菌病研究所全军真菌病重点实验室第二军医大学附属长征医院皮肤科, 上海 200003

收稿日期:2017-03-03 出版日期:2017-04-28 发布日期:2017-04-28
通讯作者: 潘炜华,E-mail:panweihua@medmail.com.cn E-mail:panweihua@medmail.com.cn
作者简介:李颖芳,女(汉族),硕士研究生在读.E-mail:liyingfang99@163.com
基金资助:
973项目（2013CB531606）；国家自然基金（31270180）

Effects of transforming growth factor on the function of macrophage against Cryptococcus neoformans

LI Ying-Fang, SANG Jun-Jun, LI Juan, JIANG Wei-Wei, LIAO Wan-Qing, PAN Wei-Hua

Department of dermatology, Changzheng Hospital, Institute of dermatology and medical mycology, Changzheng Hospital, Key laboratory of molecular medical mycology, PLA key laboratory of medical mycology, Shanghai 200003

Received:2017-03-03 Online:2017-04-28 Published:2017-04-28

摘要/Abstract

摘要：

目的研究转化生长因子对巨噬细胞吞噬和杀伤新生隐球菌（Cryptococcus neoformans）功能的影响。方法采用TGF-β干预体外活化巨噬细胞吞噬和杀伤实验，分别观察巨噬细胞吞噬率和巨噬细胞内隐球菌生长情况，采用Griess regent试剂盒检测巨噬细胞NO（Nitric oxide）生成量，并比较TGF-β干预下巨噬细胞NO生成量变化。结果 TGF-β干预后巨噬细胞吞噬率下降了43.9%，差异有统计学意义（P<0.05）。巨噬细胞在转化生长因子干预下NO生成量增加，胞内隐球菌负荷降低，差异有统计学意义（P<0.05）。结论体外细胞实验中，在TGF-β干预下活化巨噬细胞的趋化作用和吞噬能力受抑制，但巨噬细胞合成NO量增加，杀伤隐球菌能力增强。

关键词: 新生隐球菌, 转化生长因子, 巨噬细胞

Abstract:

Objective To investigate the phagocytosis and killing ability of macrophage under TGF-β intervention.Methods Using TGF-β intervene phagocytosis and killing assay of activated macrophages in vitro.Phagocytic index of macrophage and the intracellular growth of Cryptococcus neoformans was observed respectively.Nitric oxide (NO) production was detected using the Griess regent kit, and then comparing the changes of NO production in macrophages under the TGF-β intervention.Results The phagocytic index with the treatment of TGF-β decreased by 43.9%, significantly lower than control group (P<0.05).While the production of NO increased and the intracellular fungal burden significantly declined under the intervention of TGF-β (P<0.05).Conclusion Chemotaxis and phagocytosis of activated macrophage were inhibited under the intervention of TGF-β in vitro, but the NO production increased and killing ability enhanced.

Key words: Cryptococcus neoformans, transforming growth factor, macrophage

中图分类号:

R379.5

李颖芳, 桑军军, 李娟, 姜伟伟, 廖万清, 潘炜华. 转化生长因子对巨噬细胞抗新生隐球菌功能的影响[J]. 中国真菌学杂志, 2017, 12(2): 65-68.

LI Ying-Fang, SANG Jun-Jun, LI Juan, JIANG Wei-Wei, LIAO Wan-Qing, PAN Wei-Hua. Effects of transforming growth factor on the function of macrophage against Cryptococcus neoformans[J]. Chinese Journal of Mycology, 2017, 12(2): 65-68.

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转化生长因子对巨噬细胞抗新生隐球菌功能的影响

Effects of transforming growth factor on the function of macrophage against Cryptococcus neoformans

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