上海某综合性医院秋季室内外环境空气中真菌种群分布的研究

中国真菌学杂志　 2016, Vol. 11 　Issue (6): 361-365.

上海某综合性医院秋季室内外环境空气中真菌种群分布的研究

洪南, 方文捷, 刘加, 陈敏, 潘炜华, 廖万清

第二军医大学附属长征医院皮肤科, 上海长征医院皮肤病与真菌病研究所, 上海市医学真菌分子生物学重点实验室, 全军真菌病重点实验室, 上海 200003

收稿日期:2016-08-30 出版日期:2016-12-28 发布日期:2016-12-28
通讯作者: 廖万清,E-mail:liaowanqing@sohu.com;潘炜华,E-mail:panweihua@medmail.com.cn E-mail:liaowanqing@sohu.com;panweihua@medmail.com.cn
作者简介:洪南,男(汉族),硕士研究生在读.E-mail:smallhello@126.com
基金资助:
国家973项目（2013CB531601，2013CB531606）；重大传染病防治科技重大专项（2013ZXLO004612）；国家自然科学基金（81201269）；上海市科委专项（14DZ2272900）

A survey on indoor and outdoor airborne fungal species distribution in a general hospital located in Shanghai, China

HONG Nan, FANG Wen-jie, LIU Jia, CHEN Min, PAN Wei-hua, LIAO Wan-qing

Institute of dermatology and fungal disease, Key Laboratory of the army fungal disease, Department of dermatology, Changzheng Hospital, Shanghai 200003

Received:2016-08-30 Online:2016-12-28 Published:2016-12-28

摘要/Abstract

摘要：

目的监测目前上海地区某综合性医院室内外环境空气中真菌种群分布特征，为临床侵袭性真菌感染的防治提供科学依据。方法于2015年秋季以基于微生物气溶胶采集的空气采样器采样法与曝皿法随机对上海地区某综合性三甲医院的26处室内及7处室外环境进行同步空气采样，相关样本在含有氯霉素的沙氏培养基上经培养、分离、纯化及分子鉴定后，对不同采样地点真菌种群结构予以统计学分析。结果上海地区综合性三甲医院室内空气中青霉属（约24.14%）在真菌种群结构中占有明显优势，室外空气中则以分枝孢子菌属占有优势（约34.09%），青霉属在室内空气中所占比重（约24.14%）明显高于室外（11.09%）。结论虽然上海地区的医疗水平国内领先，且被调查医院已按相关规定进行了日常环境消毒工作，但被调查医院室内外空气中仍被发现存在高度多样性的真菌种群结构，且包含了常见的机会致病真菌如烟曲霉等，继续加强综合性医院室内外环境中真菌等病原微生物的监测和加强院内感染的控制是非常必要的。

关键词: 医院污染, 室内真菌, 空气采样, 真菌感染

Abstract:

Objective To monitor the characteristics of airborne fungi distribution around a Chinese general hospital during Autumn in Shanghai,for scientific basis for clinical prevention and treatment of invasive fungal infections.Methods In a randomly selected general hospital in Shanghai,collecting air samples in 26 indoor and 7 outdoor environments using cyclone-based bioaerosol sampling method and precipitation method,simultaneously.The samples were cultivated,separated and purified on the Sabouraud Agar with chloramphenicol and then identified in molecular technique.All of the data obtained was analyzed statistically.Results The genus Penicillium (24.14%) was prominent in the fungal population structure in indoor environments of the enrolled hospital.In contrast,the genus Cladosporium (34.09%) has the advantage in outdoor environments.The proportion of the genus Penicillium in indoor environments (24.14%) is significantly higher than that in outdoor environments (11.09%).Conclusion Although the level of medicine in Shanghai remains leading position in China and the investigated hospital has been conducting disinfection work according to regulations,there is still a high diversity of fungal species distribution found in the surveyed hospital,which contains common opportunistic pathogenic fungi such as Aspergillus fumigatus,etc.Therefore,continue to reinforce the research on environmental surveillance and infection control of general hospital is of significance.

Key words: hospital contamination, indoor fungi, air sampling, mycological infection

中图分类号:

R379

洪南, 方文捷, 刘加, 陈敏, 潘炜华, 廖万清. 上海某综合性医院秋季室内外环境空气中真菌种群分布的研究[J]. 中国真菌学杂志, 2016, 11(6): 361-365.

HONG Nan, FANG Wen-jie, LIU Jia, CHEN Min, PAN Wei-hua, LIAO Wan-qing. A survey on indoor and outdoor airborne fungal species distribution in a general hospital located in Shanghai, China[J]. Chinese Journal of Mycology, 2016, 11(6): 361-365.

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