隐球菌性脑膜炎小鼠模型脑组织中VEGFR表达变化的研究

中国真菌学杂志　 2016, Vol. 11 　Issue (5): 257-260.

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隐球菌性脑膜炎小鼠模型脑组织中VEGFR表达变化的研究

郑冲¹, 温海², 郭芳¹, 樊一斌³

1. 福建医科大学附属龙岩第一医院神经内科, 龙岩 364000;
2. 上海长征医院皮肤病与真菌病研究所全军真菌病重点实验室第二军医大学附属长征医院皮肤科, 上海 200003;
3. 浙江省人民医院皮肤科, 杭州 310024

收稿日期:2016-06-30 出版日期:2016-10-28 发布日期:2016-10-28
通讯作者: 樊一斌,E-mail:frankgets@sina.com E-mail:frankgets@sina.com
作者简介:郑冲,男(汉族),硕士,主治医师.E-mail:15880699252@163.com

The study of expression changes of VEGFR in mice brain tissue infected with Cryptococcus neoformans

ZHENG Chong¹, WEN Hai², GUO Fang¹, FAN Yi-bin³

1. Department of neurology, The Longyan affiliated hospital of Fujian Medical University, Longyan 364000;
2. Institute of dermatology and fungal disease, Key Laboratory of the army fungal disease, Department of dermatology, Changzheng Hospital, Shanghai 200003;
3. Department of Dermatology, Zhejiang Provincial People's Hospital, Hangzhou 310024

Received:2016-06-30 Online:2016-10-28 Published:2016-10-28

摘要/Abstract

摘要：

目的探寻隐球菌性脑膜炎小鼠模型中脑组织血管内皮生长因子受体（vascular endothelial growth factor recepter，VEGFR）在感染隐球菌后的变化规律。方法通过尾静脉接种隐球菌菌悬液的方法构建中枢神经系统隐球菌感染小鼠模型（n=30）为实验组，根据模型建立后5个时间点（6 h、12 h、24 h、48 h、72 h）分为5组，每组6只；对照组（n=6）尾静脉接种等量生理盐水。采用Western blotting技术测定小鼠脑组织中VEGFR1和VEGFR2蛋白。结果实验组小鼠脑组织中VEGFR1和VEGFR2蛋白测定结果与对照组相比有下降，差别有统计学意义。结论隐球菌性脑膜炎小鼠感染隐球菌后脑组织VEGFR表达下调，可能是隐球菌感染致脑水肿的继发保护机制。

关键词: 隐球菌性脑膜炎, 小鼠模型, 血管内皮生长因子受体

Abstract:

Objective To investigate the expression changes of the vascular endothelial growth-factor receptors (VEGFR) in the mice brain tissus after being infected by Cryptococcus neoformans.Methods Test group mice (n=30) were injected with Cryptococcus neoformans via the tail vein,while control group mice (n=6) received saline.Western blotting technology was used in 6 h,12 h,24 h,48 h,72 h to detect VEGFR1 and VEGFR2 expression changes in mice brain tissus.Rusluts Compared with the control group,VEGFR1 and VEGFR2 protein expressions in the test group were down.There was significant difference between the test group and control group (P<0.05).Conclusions VEGFR might play protective role in brain edema caused by cryptococcal meningitis.

Key words: cryptococcal meningitis, mice model, vascular endothelial growth factor recepter

中图分类号:

R379.5

郑冲, 温海, 郭芳, 樊一斌. 隐球菌性脑膜炎小鼠模型脑组织中VEGFR表达变化的研究[J]. 中国真菌学杂志, 2016, 11(5): 257-260.

ZHENG Chong, WEN Hai, GUO Fang, FAN Yi-bin. The study of expression changes of VEGFR in mice brain tissue infected with Cryptococcus neoformans[J]. Chinese Journal of Mycology, 2016, 11(5): 257-260.

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隐球菌性脑膜炎小鼠模型脑组织中VEGFR表达变化的研究

The study of expression changes of VEGFR in mice brain tissue infected with Cryptococcus neoformans

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