关键词: 烟曲霉, 硫氧还蛋白还原酶, 免疫保护作用

Abstract:

Objective To investigate the immunoprotection in mice immunized with thioredoxin reductase GliT of Aspergillus fumigatus.Methods C57BL/6 mice were randomly assigned into two groups:immunization group intramuscularly immunized with recombinant TR for two times;control group intramuscularly immunized with PBS instead of TR.Survival rate,A.fumigatus lung burdens,pathologic examination and cell count of alveolar lavage were evaluated.Results The survival rate of immunization group was 64.7% within 7 days after infection while that of control group was 0.The medians of CFUs from the lung tissues of the suvivors and the nonsuvivors were 0 and 44 (P₂₅,P₇₅ were 21,70) respectively (P<0.01).Pulmonary pathology indicated that the lungs of the dead immunized mice and control mice showed alveolar hemorrhage,inflammatory cells recruitment,focal tissue necrosis and hyphal elements throughout the entire lung.In contrast,the lung tissues of the survivors were damaged slightly and free of hyphal elements.Alveolar lavage fluid smear showed that the counting of the survival is given priority to with mononuclear cells,accounting for about 84.2%,neutrophils accounted 15.8%.The counting of the nonsurvival is given priority to with neutrophils,accounting for about 66.4%,while 33.6% mononuclear cells.Conclusion Recombinant TR of Aspergillus fumigatus can induce a modest protection in a murine model of invasive aspergillosis and might be a potential protective antigen.

Key words: Aspergillus fumigatus, thioredoxin reductase GliT, protective effect