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中国真菌学杂志 2015, Vol. 10  Issue (3): 163-167.

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一种适于丝状真菌基因RNA干扰方法的研究

张振颖1, 侯彬彬2, 刘霞2   

  1. 1. 香港大学深圳医院皮肤科, 深圳 518053;
    2. 大连医科大学附属第二医院皮肤科, 大连 116023
  • 收稿日期:2014-12-29 出版日期:2015-06-28 发布日期:2015-06-28
  • 通讯作者: 侯彬彬, E-mail:houbinbin1001@163.com;刘霞, E-mail:765124126@qq.com E-mail:houbinbin1001@163.com;765124126@qq.com
  • 作者简介:张振颖, 女 (汉族), 博士, 副主任医师.E-mail:15541192286@126.com
  • 基金资助:

    国家自然科学基金(81000699), 深圳市科创委(JCYJ20140414092023239)

An investigation on RNA-interference method applicable for the filamentous fungi

ZHANG Zhen-ying1, HOU Bin-bin2, LIU Xia2   

  1. 1. Department of Dermatology, HongKong University-Shenzhen Hospital, Shenzhen 518053;
    2. Department of Dermatology, 2nd Affiliated Hospital of Dalian Medical University, Dalian 116023
  • Received:2014-12-29 Online:2015-06-28 Published:2015-06-28

摘要:

目的 获得一种适于丝状真菌基因研究用的、由根癌农杆菌介导的RNA干扰方法。方法 采用基因重组及菌株干扰体系筛选的方法。结果 获得适于根癌农杆菌转染的重组载体PCB309-pfgrt,并将其成功用于对孢子丝菌双组份信号组氨酸蛋白激酶DRK1基因的干扰中。结论 该方法优化了根癌农杆菌的转化体系,解决了丝状真菌RNA干扰载体构建困难及干扰效率低下的难题,在丝状真菌基因功能及遗传转化研究方面具有广泛的应用前景。

关键词: 重组载体, RNA干扰, 丝状真菌, 根癌农杆菌

Abstract:

Objective To obtain a RNA-interference method mediated by Agrobacterium tumefaciens applicable for gene research on filamentous fungi.Methods Gene recombination and interference system selection were used.Results The recombinant vector PCB309-pfgrt was obtained and used in interfering the expression level of two-component DRK1 gene successfully.Conclusions This method optimized the transformation system,solving the problem of high efficient RNA interference vector construction,which has wide application prospect in genetic research in filamentous fungi.

Key words: recombinant vector, RNA-interference, filamentous fungi, Agrobacterium tumefaciens

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