不同浓度5-氨基酮戊酸对光动力方法抗白念珠菌效果的影响

中国真菌学杂志　 2015, Vol. 10 　Issue (2): 104-107.

不同浓度5-氨基酮戊酸对光动力方法抗白念珠菌效果的影响

徐辉¹, 陈虹霞², 邹先彪²

1. 解放军医学院, 北京 100853;
2. 解放军总医院第一附属医院皮肤科, 北京 100048

收稿日期:2014-12-30 出版日期:2015-04-28 发布日期:2015-04-28
通讯作者: 邹先彪,E-mail:xbzou@126.com E-mail:xbzou@126.com
作者简介:徐辉,男(汉族),硕士研究生在读,主治医师.E-mail:81764988@qq.com

The antifungal effect of 5-aminolevulinic acid photodynamic therapyat different concentrations on C.albicans

XU Hui¹, CHEN Hong-xia², ZOU Xian-biao²

1. Chinese PLA Medical School, Beijing 100853, China;
2. Department of Dernatlogy, First Affiliated Hospital of Chinese PLA General Hospital, Beijng 100048, China

Received:2014-12-30 Online:2015-04-28 Published:2015-04-28

摘要/Abstract

摘要：

目的通过测定白念珠菌内不同光敏剂浓度下生成原卟啉IX (PpIX)的水平及5-氨基酮戊酸光动力疗法 (5-ALA-PDT)对白念珠菌的抑菌率,为临床选择最佳光敏剂浓度提供理论依据。方法制备白念珠菌悬液,与ALA避光孵育,采用激光共聚焦显微镜观察PpIX产生情况,MTT法测定5-ALA-PDT对白念珠菌生长的抑制率。结果白念珠菌与ALA避光孵育后有荧光物质PpIX产生,ALA光敏剂浓度与PpIX强度呈正相关,当ALA浓度到达300 mg/mL时,PpIX强度将不再增加。对照组无荧光物质出现。结论 ALA-PDT对白念珠菌抑制效应同光敏剂浓度密切相关。这为临床ALA-PDT治疗白念珠菌疾病提供了理论依据。

关键词: 5-氨基酮戊酸, 光动力, 白念珠菌, 药物浓度

Abstract:

Objective To contribute theoretically to the selection of the optimal concentration of the photosensitizer through the determination of the level at which C.albicans generate protoporphyrin IX (PpIX) under different concentrations of the photosensitizer and of the bacteriostatic rate of 5-amino ketones pentanoic acid photodynamic therapy (5-ALA PDT).Methods C.albicans suspension was prepared and incubated with ALA in the dark.The generation of PpIX was observed under a laser confocal microscope.The inhibitory rate of 5-ALA PDT on C.albicans was determined by MTT method.Results After dark incubation,C.albicans and ALA produced fluorescent PpIX.ALA concentration was positively correlated with the intensity of PpIX.When drug concentration reached 300mmol/L,the intensity of PpIX strength ceased to increase.There was no fluorescent material in control group.Conclusions The inhibitory effect of ALA PDT on C.albicans was closely related to the concentration of the photosensitizer,which provides data for treatment of C.albicans infection.

Key words: 5-amino ketones pentanoic acid, photodynamic, C.albicans, drug concentration

中图分类号:

R379.4

徐辉, 陈虹霞, 邹先彪. 不同浓度5-氨基酮戊酸对光动力方法抗白念珠菌效果的影响[J]. 中国真菌学杂志, 2015, 10(2): 104-107.

XU Hui, CHEN Hong-xia, ZOU Xian-biao. The antifungal effect of 5-aminolevulinic acid photodynamic therapyat different concentrations on C.albicans[J]. Chinese Journal of Mycology, 2015, 10(2): 104-107.

参考文献

[1] Smijs TG,vander HR,Lugtenburg J,et al.Photodynamic treatment of the dermatophyte Trichophyton rubrum and its microconidia with porphyrin photosensitizers
[J].Photochem photobiol,2004,80(2):197-202.

[2] Soares BM,Resende MA,Silva DL,et al.In vitro photodynamic inactivation of Candida spp.growth and adhesion to buccal epithelial cells
[J].J Photochem Photobiol B,2009,94(1):65-70.

[3] Qiao JJ,Li RY,Ding YG,et al.Photodynamic therapy in the treatment of superficial mycoses:an evidence-based evaluation
[J].Mycopathologia,2010,170(5):339-343.

[4] Alexiades-Armenakas M.Aminolevulinic acid photodynamic therapy for actinickeratoses/actinic cheilitis/acne:vascular laser
[J].Dermatol Clin,2007,25(1):25-33.

[5] Wang XL,Wang HW,Guo MX,et al,Treatment of skin cancer and pre-cancer using topical ALA-PDT-A single hospital experience
[J].Photodiagnosis Photodyn Ther,2008,5(2):127-133.

[6] Wang XL,Wang HW,Guo MX,et al.Combination of immunotherapy and photodynamic therapy in the treatment of Bowenoid papulosis
[J].Photodiagnosis Photodyn Ther,2007,4(2):88-93.

[7] Wang XL,Wang HW,WangHS,et al.Topical 5-aminolevulinic acid photodynamic therapy for the treatment of urethral condylomata acuminata
[J].Br J Dermatol,2004,151(4):880-885.

[8] Kacerovska D,Pizinger K,KumpovaM,et al.Genital warts treated by photodynamic therapy
[J].Skinmed,2007,6(6):295-297.

[9] NestorMS.The use of photo dynamic therapy for treatment of acne vulgaris
[J].Dermatol Clin,2007,25(1):47-57.

[10] Horfelt C,Stenquist B,Larko O,et al.Photodynamic therapy for acne vulgaris:a pilot study of the dose-response and mechanism of action
[J].Acta Derm Venereol,2007,87(4):325-329.

[11] 麦浩山.铁螯合剂增强 5-氨基酮戊酸光动力诊疗进展
[J].北方药学,2014,11(5):103-104.

[12] 梁义,卢丽明,陈勇,等.5-氨基酮戊酸光动力疗法对白念珠菌抑制效应的实验研究
[J].海南医学,2013,24(10):1405-1406.

[13] YUANTian-hong,WANG Ming-yong,WU Sheng-wei,et al.Experimental study on correlation betweendimorphismand virulence in Candida albicans
[J].Chin J Derm Venereol,2004,1(1):580-581(in Chinese).

[14] LevitzSM,Diamond RD.A rapid eolorimetric assay of fungal viability with the tetrazolium salt MTT
[J].Infect Dis,1985,152(5):938-945.

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