5%阿莫罗芬甲涂剂治疗兔甲真菌病的疗效观察

中国真菌学杂志　 2013, Vol. 8 　Issue (5): 269-273.

5%阿莫罗芬甲涂剂治疗兔甲真菌病的疗效观察

杨连娟^1,2, 莫小辉², 杨虹², 高志琴², 张楚光², 余茜², 朱红梅¹, 温海¹

1. 上海长征医院皮肤病与真菌病研究所全军真菌病重点实验室第二军医大学附属长征医院皮肤科, 上海 200003;
2. 上海市皮肤病医院, 上海 200050

收稿日期:2013-05-27 出版日期:2013-10-28 发布日期:2013-10-28
通讯作者: 温海,E-mail:wenhai98@sohu.com E-mail:wenhai98@sohu.com
作者简介:杨连娟,女 (汉族),博士研究生在读,副主任医师.E-mail:lianjuan_yang@aliyun.com

Efficacy evaluation of 5% amorolfine nail lacquer topical treatment in the rabbits’ onychomycosis model

YANG Lian-juan^1,2, MO Xiao-hui², YANG Hong², GAO Zhi-qin², ZHAGN Chu-guang², YU Qian², ZHU Hong-mei¹, WEN Hai¹

1. Institute of dermatology and fungal disease, Key Laboratory of the army fungal disease, Department of dermatology, Changzheng Hospital, Shanghai 200003;
2. Shanghai Dermatology hospital, Shanghai 200050

Received:2013-05-27 Online:2013-10-28 Published:2013-10-28

摘要/Abstract

摘要： 目的建立甲真菌病动物模型,观察局部使用5%阿莫罗芬甲涂剂治疗模型甲的疗效。方法我们将须癣毛癣菌接种在新西兰白兔的后肢趾甲上,构建兔甲真菌病模型,感染成功后予组织病理学检查观察真菌在甲内的生长形态和方式,并外用5%阿莫罗芬甲涂剂治疗模型甲4周,用真菌学半定量培养评估其疗效。结果在免疫抑制的情况下,须癣毛癣菌感染新西兰白兔的甲真菌病模型构建成功,接种1周后趾甲近端即出现白色、淡黄色云雾状的改变,与人甲真菌病的临床改变相似。组织学可以看到有隔分支菌丝穿入甲板,到达甲床。接种后2、4、6周,模型甲总体感染率分别为52.78%、77.78%和83.33%。外用5%阿莫罗芬甲涂剂治疗2、4周时真菌学有效率分别是22.22%和66.67%。结论成功建立了甲真菌病的动物模型,并用此模型评估了外用抗真菌药的疗效,5%阿莫罗芬甲涂剂治疗兔甲真菌病4周的真菌学有效率为66.67%。

关键词: 甲真菌病, 新西兰白兔, 组织学检查, 真菌培养, 5%阿莫罗芬甲涂剂

Abstract: Objective To establish an animal model of onychomycosis and evaluate the efficacy of 5% amorolfine nail lacquer in the model.Methods We applied Trichophyton mentagrophytes to the nails of the hind limbs of rabbits to establish an experimental,in vivo model of onychomycosis. The nails were taken from the rabbits' feet to histopathological examination after infection success. The growth form of the fungi was evaluated histopathologically. Then 5% amorolfine nail lacquer was applied to the nail for 4 weeks and the efficacy was evaluated by the fungal semiquantitative culture method.Result We successed to establish the onychomycosis model of rabbits that underwent steroid treatment. A cloudy white or pallide-flavens appearance like that of human onychomycosis was observed in the infected nails after inoculation one week. On histopathological examination, hyphae of T.mentagrophytes were seen to penetrate the nail plate, reached the nail bed. The total infection rate at 2, 4, and 6 weeks after inoculation was 52.78%,77.78% and 83.33%, respectively. The culture-positive rate at 2 and 4 weeks after topical treatment was 22.22% and 66.67%, respectively.Conclusion We successed to establish an onychomycosis model in using rabbits and evaluated the efficacy of a topical antifungal agent in this model. The efficacy of 5% amorolfine nail lacque in this model was 66.67% after 4 weeks.

Key words: onychomycosis, rabbit, histopathological examination, fungal culture, 5% amorolfine nail lacque

中图分类号:

R756.4

杨连娟, 莫小辉, 杨虹, 高志琴, 张楚光, 余茜, 朱红梅, 温海. 5%阿莫罗芬甲涂剂治疗兔甲真菌病的疗效观察[J]. 中国真菌学杂志, 2013, 8(5): 269-273.

YANG Lian-juan, MO Xiao-hui, YANG Hong, GAO Zhi-qin, ZHAGN Chu-guang, YU Qian, ZHU Hong-mei, WEN Hai. Efficacy evaluation of 5% amorolfine nail lacquer topical treatment in the rabbits’ onychomycosis model[J]. Chinese Journal of Mycology, 2013, 8(5): 269-273.

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