儿童孢子丝菌病的临床分析及致病菌的基因型检测

中国真菌学杂志　 2013, Vol. 8 　Issue (2): 74-77,128.

儿童孢子丝菌病的临床分析及致病菌的基因型检测

吕莎¹, 李福秋¹, 金菊花¹, 刘晓明²

1. 吉林大学第二医院皮肤科, 长春 130041;
2. 大连医科大学第一医院皮肤科, 大连 116000

收稿日期:2012-08-13 出版日期:2013-04-28 发布日期:2013-04-28
通讯作者: 李福秋,E-mail:Lifuqiu@medmail.com.cn E-mail:Lifuqiu@medmail.com.cn
作者简介:吕莎,女 (汉族),硕士,住院医师.E-mail:tuzicaoye@163.com
基金资助:
吉林省自然科学基金(201215062)

Clinical analysis and genotype identification of pathogenic strains of children’s sporotrichosis in Fuyu county, Jilin Province

LV Sha¹, LI Fu-qiu¹, JIN Ju-hua¹, LIU Xiao-ming²

1. Department of Dermatology, the Second Hospital of Jilin University, Changchun 130041;
2. The First Hospital of Dalian medical University, Dalian 116000

Received:2012-08-13 Online:2013-04-28 Published:2013-04-28

摘要/Abstract

摘要： 目的对吉林省扶余县35例儿童孢子丝菌病进行临床分析并对部分菌株进行基因测定,了解该地区孢子丝菌病的致病菌菌株基因是否发生变异而致致病力增强及发病率增高。方法收集2011年1月1日~4月30日来自扶余县就诊于我科并确诊为孢子丝菌病的患儿35例,进行临床分析;从中选取8株菌,对其核糖体保守区及内转录间隔区(ITS)、非转录间隔区(NTS)区进行基因测定,了解基因水平上的异同。结果 8株孢子丝菌菌株中5株NTS区可见较大范围的碱基缺失。结论吉林省扶余县孢子丝菌菌株可能由于NTS区碱基较大范围缺失而致致病力增强,导致该地区短时间内发病率增高。

关键词: 申克孢子丝菌, NTS, 致病力

Abstract: Objective To make the clinical analysis of children's Sporothrix schenckii infections and genic identification of partial strains in Fuyu county,Jilin Province. And to understand whether the strains in this area had stronger pathogenicity because of genic differences by comparing the gene sequences of these strains with those of Sporothrix schenckii strains from another county in Jilin Province.Methods To analyze the clinical characteristics, 35 cases of children's sporotrichosis in our clinic from January 1 to Apirl 30,2011 were enrolled. The genic identifications of ribosome-conservative spacer, ITS and NTS of 8 of the strains were performed.Results Comparing with the standard strain, the NTS of five strains which caused Sporothrix schenckii infections in Fuyu coutry have the loss of many bases. Among them, four strains have the non-contiguous loss of 10 bases from exactly the same positions 208 to 232bp; the other one strain have the non-contiguous loss of 21 bases from the position 208 to 249 bp.Conclusions The strains which resulted in the higher incidence of sporotrichosis in Fuyu county might have stronger pathogenicity because of the bases loss in NTS.

Key words: Sporothrix schenckii, NTS, pathogenicity

中图分类号:

R756.6

吕莎, 李福秋, 金菊花, 刘晓明. 儿童孢子丝菌病的临床分析及致病菌的基因型检测[J]. 中国真菌学杂志, 2013, 8(2): 74-77,128.

LV Sha, LI Fu-qiu, JIN Ju-hua, LIU Xiao-ming. Clinical analysis and genotype identification of pathogenic strains of children’s sporotrichosis in Fuyu county, Jilin Province[J]. Chinese Journal of Mycology, 2013, 8(2): 74-77,128.

[1]	黄春阳, 潘开素, 李秀楹, 曹存巍, 郑艳青. Calcineurin A基因对马尔尼菲篮状菌致病力的影响[J]. 中国真菌学杂志, 2020, 15(3): 138-144.
[2]	张静云, 佘晓东, 刘维达. 白念珠菌糖代谢与毒力[J]. 中国真菌学杂志, 2020, 15(1): 61-64.
[3]	赵林, 王林会, 郑方亮, 侯彬彬. 申克孢子丝菌STE20基因RNA干扰菌株的构建方法[J]. 中国真菌学杂志, 2019, 14(3): 129-133.
[4]	张一男, 刘晓娜, 杨晶, 刘玥姣, 石铭, 薛文成. 子宫颈感染孢子丝菌1例[J]. 中国真菌学杂志, 2019, 14(1): 47-49.
[5]	张振颖, 胡媛媛, 陈凯丽, 侯彬彬. 初探Ste-20激酶在调控孢子丝菌双相生长过程中的作用[J]. 中国真菌学杂志, 2018, 13(5): 268-271,261.
[6]	宁心强, 魏金瑛, 郑艳青, 梁浩, 曹存巍. SakA对马尔尼菲篮状菌药物应激及致病力的影响[J]. 中国真菌学杂志, 2018, 13(2): 65-70.
[7]	赵莉佩, 崔岩, 李珊山. 申克孢子丝菌基因分型的研究现状与展望[J]. 中国真菌学杂志, 2016, 11(5): 316-320.
[8]	万雪, 贺丹, 王丽. 申克孢子丝菌毒力因子及宿主抗感染免疫的研究进展[J]. 中国真菌学杂志, 2013, 8(5): 307-310.
[9]	吕莎, 李福秋, 金菊花, 刘晓明. 1例皮肤播散型孢子丝菌病的分离菌株的基因鉴定[J]. 中国真菌学杂志, 2013, 8(1): 35-36.
[10]	胡素泉, 郭宁如, 蔡晴, 宋学东, 李筱芳, 吴绍熙. 1株多重耐药申克孢子丝菌感染的治疗[J]. 中国真菌学杂志, 2012, (4): 228-229.
[11]	李芸, 毛笑非, 杜伟, 刘跃华, 孙秋宁. 鼻部固定型孢子丝菌病1例[J]. 中国真菌学杂志, 2012, (2): 98-100.
[12]	李聪慧, 冉玉平, 王鹏, 代亚玲. 发生于双侧腰部的孢子丝菌病1例[J]. 中国真菌学杂志, 2012, 7(1): 31-32.
[13]	王爱平, 高那, 刘伟, 万喆, 陈伟, 涂平, 李若瑜. 伊曲康唑联合特比萘芬治疗孢子丝菌病疗效及其对病原真菌体外抗菌活性研究[J]. 中国真菌学杂志, 2011, 6(1): 5-9.
[14]	蔡晴, 宋洋, 沈永年, 吕桂霞, 陈伟, 李珊山, 刘维达. 申克孢子丝菌菌丝相至酵母相转化的实验研究[J]. 中国真菌学杂志, 2010, 5(5): 273-276.
[15]	原英, 赵颖, 马依努尔, 徐英春, 王澎. 申克孢子丝菌感染诊治1例[J]. 中国真菌学杂志, 2010, 5(3): 172-174.