烟曲霉在不同温度和营养条件下的生长特性初探

中国真菌学杂志　 2013, Vol. 8 　Issue (1): 6-9.

烟曲霉在不同温度和营养条件下的生长特性初探

张晋卿, 刘伟, 谭静文, 万喆, 李若瑜

北京大学第一医院皮肤性病科北京大学真菌与真菌病研究中心皮肤病分子诊断北京市重点实验室, 北京 100034

收稿日期:2012-12-10 出版日期:2013-02-28 发布日期:2013-02-28
通讯作者: 李若瑜,E-mail:mycolab@126.com E-mail:mycolab@126.com
作者简介:张晋卿,女(汉族),博士研究生在读.E-mail:jinqing.chung@gmail.com
基金资助:
国家自然科学基金重点项目(30930006);国家自然科学基金(30970131);北京市自然科学基金(7102149);教育部"新世纪优秀人才支持计划"(NCET-10-0198)

Analysis of the growth characteristics of Aspergillus fumigatus in different temperature and nutrient media

ZHANG Jin-qing, LIU Wei, TAN Jing-wen, WAN Zhe, LI Ruo-yu

Department of Dermatology, Peking University First Hospital, Research Center for Medical Mycology, Beijing Key Laboratory of Molecular Diagnosis on Dermatoses, Peking University, Beijing 100034, China

Received:2012-12-10 Online:2013-02-28 Published:2013-02-28

摘要/Abstract

摘要： 目的确定烟曲霉的最适生长温度、最高生长温度及致死温度。方法生长曲线法用来确定培养烟曲霉的最适宜培养基,并测定其最适生长温度和最高生长温度;菌落计数法用来确定其致死温度。结果 1)酵母浸膏培养基(yeast extract medium,YG)是测定烟曲霉生长曲线的最适宜培养基;2)在YG培养基中,37℃是烟曲霉的最适宜生长温度,52℃为其最高生长温度。随着温度的升高,菌株的生长速度逐渐减慢;3)65℃处理180 min或70℃处理120 min能使烟曲霉完全失活。结论在YG培养基中,37℃是烟曲霉的最适宜生长温度,52℃为其最高生长温度;烟曲霉的致死条件为65℃处理180 min或70℃处理120 min。

关键词: 烟曲霉, 温度, 生长曲线

Abstract: Objective To determine the growth characteristics of Aspergillus fumigatus in different nutrient media and temperature.Methods Growth curves were used to determine the optimum medium, optimum and maximum growth temperatures for two strains of Aspergillus fumigatus:colony counts were used to determine the fatal temperatures for them.Results Three different nutrient media, yeast extract medium (YG), minimal medium (MM) and yeast nitrogen base (YNB), were tested. YG medium was the optimum medium for measuring the growth curves of Aspergillus fumigatus; In YG medium, the optimum growth temperature for Aspergillus fumigatus was 37℃, and the maximum growth temperature was 52℃. The slower growth rates of strains were observed with gradually elevated temperature. Unexpectedly, the responses of these two strains to high temperature were different:one strain's growth became slower markedly at 48℃ than at 37℃ during log phase, while the other one grew at 48℃ as well as at 37℃ during log phase. The strains of Aspergillus fumigatus were dead when exposed to 65℃ lasting more than 180 min or 70℃ lasting more than 120 min.Conclusions The optimum and the maximum growth temperature for Aspergillus fumigatus were 37℃ and 52℃, respectively.The fatal condition for Aspergillus fumigatus was 65℃ lasting more than 180 min or 70℃ lasting more than 120 min.

Key words: Aspergillus fumigatus, temperature, growth curve

中图分类号:

R379.6

张晋卿, 刘伟, 谭静文, 万喆, 李若瑜. 烟曲霉在不同温度和营养条件下的生长特性初探[J]. 中国真菌学杂志, 2013, 8(1): 6-9.

ZHANG Jin-qing, LIU Wei, TAN Jing-wen, WAN Zhe, LI Ruo-yu. Analysis of the growth characteristics of Aspergillus fumigatus in different temperature and nutrient media[J]. Chinese Journal of Mycology, 2013, 8(1): 6-9.

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