小鼠隐球菌性脑膜炎模型AQP4与脑水肿关系的实验研究

中国真菌学杂志　 2012, Vol. 7 　Issue (6): 335-338.

小鼠隐球菌性脑膜炎模型AQP4与脑水肿关系的实验研究

樊一斌¹, 温海², 朱红梅², 徐红², 赵瑾²

1. 浙江省人民医院皮肤科,杭州 310024;
2. 上海长征医院皮肤病与真菌病研究所全军真菌病重点实验室第二军医大学附属长征医院皮肤科,上海 200003

收稿日期:2012-08-28 出版日期:2012-12-28 发布日期:2012-12-28
通讯作者: 温海 E-mail:wenhai98@sohu.com
作者简介:樊一斌,男 (汉族),博士,住院医师.E-mail:frankgets@sina.com
基金资助:
国家自然科学基金 (30670118)

The relationship between AQP4 and brain edema of cryptococcal meningitis model in mice

FAN Yi-bin¹, WEN Hai², ZHU Hong-mei², XU Hong², ZHAO Jin²

1. Department of Dermatology,Zhejiang Provincial People's Hospital,Hangzhou 310024;
2. Institute of dermatology and fungal disease,Key Laboratory of the army fungal disease,Department of dermatology,Changzheng Hospital,Shanghai 200003

Received:2012-08-28 Online:2012-12-28 Published:2012-12-28

摘要/Abstract

摘要：

目的探讨隐球菌感染中枢神经系小鼠模型AQP4与脑水肿的关系。方法尾静脉接种隐球菌构建小鼠中枢神经系感染模型,随机分为实验组和对照组,免疫抑制后实验组小鼠尾静脉注射隐球菌菌悬液,对照组注射等量生理盐水。两组均采用Western blotting技术于6 h、12 h、24 h、48 h、72 h动态检测小鼠脑组织中AQP4蛋白表达变化。结果小鼠隐球菌中枢神经系统感染后,脑组织中AQP4蛋白表达24 h开始上升,48 h达峰值,在24 h和48 h与对照组比较,实验组的标准化AQP4蛋白表达水平显著增加,与对照组差异有统计学意义 (P<0.05)。结论 AQP4对隐球菌中枢神经系统感染所致脑水肿具有保护性作用。

关键词: 新生隐球菌, 小鼠模型, 脑水肿, 水通道蛋白-4

Abstract:

Objective To investigate the relationship between AQP4 and brain edema in murine model with central nervous system cryptococcal infection.Methods Mice were randomLy devided into two groups.Test group mice were injected with Cryptococcus neoformans via the tail vein,while control group mice received saline. Western Blotting technology was used in 6 h, 12 h, 24 h, 48 h, 72 h to detected AQP4 protein expression changes in the brain tissue of mice.Results Comared with the control group, AQP4 protein expression in the test group began to rise at 24 h and reached maximum at 48 h.Conclusions AQP4 play protective role in cerebral edema caused by Cryptococcal meningitis.

Key words: Cryptococcus neoformans, mice model, brain edema, AQP-4

中图分类号:

R 379.5

樊一斌, 温海, 朱红梅, 徐红, 赵瑾. 小鼠隐球菌性脑膜炎模型AQP4与脑水肿关系的实验研究[J]. 中国真菌学杂志, 2012, 7(6): 335-338.

FAN Yi-bin, WEN Hai, ZHU Hong-mei, XU Hong, ZHAO Jin. The relationship between AQP4 and brain edema of cryptococcal meningitis model in mice[J]. Chinese Journal of Mycology, 2012, 7(6): 335-338.

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