近平滑念珠菌的形态转换观察

中国真菌学杂志　 2010, Vol. 5 　Issue (2): 87-91.

近平滑念珠菌的形态转换观察

王淼淼, 吕雪莲, 刘泽虎, 吕桂霞, 沈永年, 刘维达

中国医学科学院北京协和医学院皮肤病研究所真菌科, 南京, 210042

收稿日期:2009-10-15 出版日期:2010-04-28 发布日期:2010-04-28
通讯作者: 刘维达,E-mail:liumyco@hotmail.com E-mail:liumyco@hotmail.com
作者简介:王淼淼,女(汉族),博士研究生在读.E-mail:wwssyy535@sohu.com

Phenotype switching of Candida parapsilosis

WANG Miao-miao, LV Xue-lian, LIU Ze-hu, LV Xue-lian, SHEN Yong-nian, LIU Wei-da

Department of Mycology, Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing 210042, China

Received:2009-10-15 Online:2010-04-28 Published:2010-04-28

摘要/Abstract

摘要： 目的观察近平滑念珠菌在不同培养基的形态转换现象,以及温度对其形态转换的影响。方法收集近平滑念珠菌正常人皮肤携带株及临床致病株和标准株,接种于改良Lee培养基和含桃红B的YPD培养基,观察其不同形态转换,以及温度变化对光滑(W)与皱褶(O)形态转换的影响。结果近平滑念珠菌在Lee培养基和含桃红B的YPD培养基上,均可以出现多种形态以及一定频率W-O转换现象。在观察W向O形态转换过程中发现,与25℃培养温度相比,37℃条件下光滑菌落形态占更多的比例。结论近平滑念珠菌体外培养时存在形态转换及W-O转换现象,且于37℃时更易保持光滑形态。含桃红B的YPD培养基也可以用于基本的W-O形态转换观察。

关键词: 念珠菌,近平滑, 形态转换, 光滑, 皱褶

Abstract: Objective To investigate the phenotype switching of Candida parapsilosis on different medium and the effect of temperature.Methods Nine isolates of C.parapsilosis collected from the skin of healthy people and clinic patients were vauinated on modified Lee’s medium and YPD medium supplemented with phloxine B.Phenotype switching and the effect of different temperature were studied.Results High-frequency phenotypic switching and white opaque switch of C.parapsilosis were observed on both Lee’s medium and YPD medium supplemented with phloxine B.The proportion of opaque colony under 37℃ was lower than that under 25℃ on Lee’s medium.Conclusions C.parapsilosis is capable of high-frequency phenotypic switching and white-opaque transition with stable smooth form at 37℃.

Key words: Candida parapsilosis, phenotype switching, white, opaque

中图分类号:

R379.4

王淼淼, 吕雪莲, 刘泽虎, 吕桂霞, 沈永年, 刘维达. 近平滑念珠菌的形态转换观察[J]. 中国真菌学杂志, 2010, 5(2): 87-91.

WANG Miao-miao, LV Xue-lian, LIU Ze-hu, LV Xue-lian, SHEN Yong-nian, LIU Wei-da. Phenotype switching of Candida parapsilosis[J]. Chinese Journal of Mycology, 2010, 5(2): 87-91.

参考文献

[1] Slutsky B,Staebell M,Anderson J,et al."White-opaque transition":a second high-frequency switching system in Candida albicans[J].J Bacteriol,1987,169 (1):189-197.
[2] Kavanagh K.Medical mycology:cellular and molecular techniques[M].New York:John Wiley & Sons,Ltd,2007.
[3] Pfaller MA,Diekema DJ.Epidemiology of invasive candidiasis:a persistent public health problem[J].Clin Microbiol Rev,2007,20(1):133-163.
[4] Mokaddas EM,Al-Sweih NA,Khan ZU.Species distribution and antifungal susceptibility of Candida bloodstream isolates in Ku-wait:a 10-year study[J].J Med Microbiol,2007,56 (Pt 2):255-259.
[5] Soll DR.High-frequency switching in Candida albicans[J].Clin Microbiol Rev,1992,5 (2):183-203.
[6] Laffey SF,Butler G.Phenotype switching affects biofilm forma-tion by Candida parapsilosis[J].Microbiology,2005,151 (Pt 4):1073-1081.
[7] Lockhart SR,Pujol C,Daniels K J,et al.In Candida albicans,white-opaque switchers are homozygous for mating type[J].Genetics,2002,162 (2):737-745.
[8] Miller MG,Johnson AD.White-opaque switching in Cartdida albicans is controlled by mating-type locus homeodomain proteins and allows efficient mating[J].Cell,2002,110(3):293-302.
[9] Enger L,Joly S,Pujol C,et al.Cloning and characterization of a complex DNA fingerprinting probe for Candida parapsilosis[J].J Clin Microbiol,2001,39(2):658-4569.
[10] Logue ME,Wong S,Wolfe KH,et al.A genome sequence survey shows that the pathogenic yeast Candida parapsilosis has a defective MTLa1 allele at its mating type locus[J].Eukaryot Cell,2005,4(6):1009-1017.
[11] Lachke SA,Lockhart SR,Daniels KJ,et al.Skin facilitates Candida albicans mating[J].Infect Immun,2003,71 (9):4970-4976.
[12] Kvaal CA,Srikantha T,Soll DR.Misexpression of the whitephase-specific gene WH11 in the opaque phase of Candida albicans affects switching and virulence[J].Infect Immun,1997,65(11):4468-4475.
[13] Miller NS,Dick JD,Merz WG.Phenotypic switching in Candida lusitaniae on copper sulfate indicator agar:association with amphotericin B resistance and filamentation[J].J Clin Microbiol,2006,44 (4):1536-1539.
[14] Lachke SA,Joly S,Daniels K,et al,Phenotypic switching and filamentation in Candida glabrata[J].Microbiol,2002,148(Pt 9):2661-2674.