关键词: 大蒜素, 白念珠菌, 生物膜

Abstract: Objective To investigate thein vitroeffects of allicin onCandida albicansbiofilms.Methods MTT assay was performed to determine the effects of allicin on Candida albicans biofilms formation and cells adhesion.Serum germ tube counting was used to evaluate the effects of allicin on germ tube formation of Candida albicans.Results The inhibition rate of allicin against Candida albicans biofilms formation was (23.0±1.1)% by 4 μg/mL and (95.6±0.3)% by 64 μg/mL.Allicin was active against different stages of biofilms,with (88.5±0.5)% 、(63.3±0.8)% and (52.3±1.1)% inhibition rate when 32 μg/mL allicin was added at 0 h、12 h and 48 h during the incubation period,respectively.Allicins from 4 μg/mL to 32 μg/mL showed distinct inhibitive effects on adhesion to Candida albicans cultured for 30 mins、60 mins、90 mins and 120 mins (P < 0.05).The rate of germ tube formation was (91.2±16)% in the absence of allicin,whereas (2.2±1.2)% in 64 μg/mL allicin group.Conclusions Allicin was demonstrated to had potent activity against Candida albicans biofilms in vitro.

Key words: allicin, Candida albicans, biofilm