覆盆子提取物联合唑类药物抗真菌活性研究

中国真菌学杂志　 2012, Vol. 7 　Issue (1): 4-7.

覆盆子提取物联合唑类药物抗真菌活性研究

张石群¹, 林辉², 苗琦¹, 刘伟², 曹永兵^1,2, 姜远英^1,2

1. 第二军医大学药学院药理学教研室, 上海200433;
2. 福建中医药大学药学院中药教研室, 福州350108

收稿日期:2011-11-24 出版日期:2012-02-28 发布日期:2012-02-28
通讯作者: 曹永兵,E-mail:ybcao@vip.sina.com;姜远英,E-mail:jiangyy@smmu.edu.cn E-mail:jiangyy@smmu.edu.cn
作者简介:张石群,男(汉族),硕士研究生在读.E-mail:zhang- shiqun1987@163.com;林辉,男(汉族),硕士研究生在读.E-mail: nuLi60tian@163.com

Combination antifungal activity of ethanol extracts from Rubus idaeus and antifungal azoles

ZHANG Shi-qun¹, LIN Hui², MIAO Qi¹, LIU Wei², CAO Yong-bing^1,2, JIANG Yuan-ying^1,2

1. Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433;
2. Department of Chinese medicine, School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108

Received:2011-11-24 Online:2012-02-28 Published:2012-02-28

摘要/Abstract

摘要： 目的探讨中药覆盆子提取物联合唑类药物的体外抗真菌作用。方法采用CLSI公布的M27-A方案微量液基稀释法和棋盘式微量稀释法,测定覆盆子提取物单用及联合唑类药物对不同念珠菌的MIC值和FICI指数。结果覆盆子不同溶液提取物与氟康唑均表现出协同关系,以覆盆子醇提物为例,单用对念珠菌的MIC₈₀测定值范围主要集中在0.16～1.25 mg/mL,与氟康唑合用后表现出协同关系(FICI≤0.5),且MIC₈₀测定值范围降至0.01～0.04 mg/mL;合用后的氟康唑抗真菌活性也明显增强。另外,覆盆子醇提物与不同唑类药物合用后均有协同关系,其MIC₈₀测定值由单用时大于10 mg/mL降至0.04 mg/mL。结论覆盆子醇提物和唑类药物单用时对耐药念珠菌的抑菌作用较弱,但二者合用后表现出明显的协同关系,对耐药念珠菌的抑菌作用明显增强。

关键词: 覆盆子, 醇提物, 体外抗真菌活性, 部分抑菌浓度指数(FICI)

Abstract: Objective To investigate the antifungal activity of extracts from Rubus idaeus combined with azole drugs against Candida spp.in vitro.Methods The minimum inhibitory concentration(MIC),the fractional inhibitory concentration index(FICI) of extracts from Rubus idaeus and combination with azole drugs against Candida spp.were determined by M27-A Microdilution Method based on CLSI.Results All extracts from Rubus idaeus by different solution methods showed collaboration with fluconazole.For example,the MIC₈₀ of ethanol extracts from Rubus idaeus against Candida spp.mainly concentrated on 0.16~1.25 mg/mL,while combined with fluconazole showed the collaboration(FICI≤0.5),and the MIC₈₀ reduced to 0.01~0.04 mg/mL.The antifungal effect of fluconazole against Candida spp.was also strengthened.Besides,the MIC₈₀s of ethanol extracts from Rubus idaeus were also reduced from 10 mg/mL to 0.04 mg/mL when being combined with other azole drugs.Conclusion The antifungal effect of ethanol extracts from Rubus idaeus or azole drugs against resistant Candidas was weaker,while the combination showed clear collaboration and the effect against resistant Candida spp.significantly enhanced.

Key words: Rubus idaeus, ethanol extracts, in vitro antifungal activity, fractional inhibitory concentration index(FICI)

中图分类号:

R379

张石群, 林辉, 苗琦, 刘伟, 曹永兵, 姜远英. 覆盆子提取物联合唑类药物抗真菌活性研究[J]. 中国真菌学杂志, 2012, 7(1): 4-7.

ZHANG Shi-qun, LIN Hui, MIAO Qi, LIU Wei, CAO Yong-bing, JIANG Yuan-ying. Combination antifungal activity of ethanol extracts from Rubus idaeus and antifungal azoles[J]. Chinese Journal of Mycology, 2012, 7(1): 4-7.