域发喷剂敷料对糠秕马拉色菌体外抑菌活性的实验研究

中国真菌学杂志　 2023, Vol. 18 　Issue (1): 19-23,41.

域发喷剂敷料对糠秕马拉色菌体外抑菌活性的实验研究

高瑞佳¹, 刘士瑞², 孙瑾鹏³, 樊靖华¹, 李福秋²

1. 西安市中心医院, 西安 710004;
2. 吉林大学第二医院, 吉林 130041;
3. 大连皮肤病医院, 大连 116021

收稿日期:2021-08-02 发布日期:2023-03-17
通讯作者: 李福秋,E-mail:lifuqiu1234@126.com E-mail:lifuqiu1234@126.com
作者简介:高瑞佳,女(汉族),硕士,住院医师.E-mail:136772749@qq.com

Experimental study on the in vitro antibacterial activity of Medi hair spray dressing against Malassezia

GAO Ruijia¹, LIU Shirui², SUN Jinpeng³, FAN Jinghua¹, LI Fuqiu²

1. Xi'an Central Hospital, Xi'an 710004, China;
2. The Second Hospital of Jilin University, Jilin 130041, China;
3. Dalian Dermatology Hospital, Dalian 116021, China

Received:2021-08-02 Published:2023-03-17

摘要/Abstract

摘要： 目的采用琼脂平板扩散法 (打孔法)和微量液基稀释法两种体外药敏实验方法,观察域发喷剂敷料及其主要成分(微生物源性抗菌肽)对头皮脂溢性皮炎常见的马拉色菌体外抑菌活性。方法不同浓度域发喷剂敷料、微生物源性抗菌肽为实验组,采乐洗剂(浓度2%酮康唑洗剂)、域发喷剂敷料空白溶剂为对照组,用琼脂平板扩散法 (打孔法)和微量液基稀释法测试体外对马拉色菌的抑制作用,同时测定MIC(最小抑菌浓度)值。结果域发喷剂敷料、微生物源性抗菌肽、采乐洗剂均对马拉色菌有不同程度的体外抑制作用,其抑菌效果与药物浓度呈正相关。域发喷剂敷料的MIC值为0.977 μL/mL、微生物源性抗菌肽的MIC值为3.907 μL/mL。结论域发喷剂敷料安全性高,不易产生耐药性,副作用少,有较好地抑制马拉色菌作用,使用方便,可用于临床上头皮脂溢性皮炎的治疗。

关键词: 域发喷剂敷料, 脂溢性皮炎, 马拉色菌, 微生物源性抗菌肽, 体外抑菌, MIC

Abstract: Objective Two in vitro drug sensitivity test methods, ager plate diffusion method (punch method) and microliquid-based dilution method, were used to observe the in vitro antibacterial activity of Medi hair spray dressing and its main components(Microbial antimicrobial peptide) against common Malassezia on the surface of seborrheic dermatitis lesions of the head. Methods Medi Hair Spray Dressing with different concentration, microbial antimicrobial peptide were used as the experimental group, and Caile lotion (concentration 2% ketoconazole lotion), blank solvent of Medi Hair Spray Dressing were used as the control group,and the diameters of the inhibition zone were compared in each group. The inhibitory effect on Malassezia in vitro was tested by agar plate diffusion method (punch method) and microdilution method, and the MIC (minimum inhibitory concentration) values were also determined. Results Medi Hair Spray Dressing and microbial antimicrobial peptide inhibited Malassezia to varying degrees in vitro,and the effect was positively correlated with drug concentration. Medi Hair Spray Dressing MIC was 0.977 μL/mL;microbial antimicrobial peptide MIC was 3.907 μL/mL. Conclusion Medi Hair Spray Dressing is safe,and has a better inhibition of Malassezia effect.

Key words: Medi Hair Spray Dressing, seborrheic dermatitis, Malassezia, microbial antimicrobial peptide, in vitro antifungal activity, MIC

中图分类号:

R379.9

高瑞佳, 刘士瑞, 孙瑾鹏, 樊靖华, 李福秋. 域发喷剂敷料对糠秕马拉色菌体外抑菌活性的实验研究[J]. 中国真菌学杂志, 2023, 18(1): 19-23,41.

GAO Ruijia, LIU Shirui, SUN Jinpeng, FAN Jinghua, LI Fuqiu. Experimental study on the in vitro antibacterial activity of Medi hair spray dressing against Malassezia[J]. Chinese Journal of Mycology, 2023, 18(1): 19-23,41.

参考文献

[1] 郑晓欢,郑荣昌,温炬, 等. 马拉色菌常见相关疾病的研究进展[J].国际医药卫生导报, 2018, 24(15):2227-2229.
[2] PARK H, OH J, LEE Y, et al. Inflammasome-mediated inflammation by Malassezia in human keratinocytes:A comparative analysis with different strains[J]. Mycoses, 2020:64(3):292-299.
[3] GAITANIS G, MAGIATIS P, HANTSCHKE M, et al. The Malassezia genus in skin and systemic diseases[J]. Clin Microbiol Rev, 2012, 25(1):106-141.
[4] 周捷, 宁静, 于伟,等. 从专利角度分析微生物源抗菌肽研发现状及研发思路[J]. 沈阳药科大学学报, 2019, 36(8):750-754.
[5] 奚锦,王文佳, 罗绂重,等. 抗菌肽的研究现状[J]. 贵阳中医学院学报, 2019, 41(6):88-90.
[6] AGARWAL G, GABRANI R. Antiviral peptides: identification and validation[J]. Int J Pept Res Ther, 27(1):149-168. [7] MERCER D, TORRES M, DUAY S, et al. Antimicrobial susceptibility testing of antimicrobial peptides to better predict efficacy[J]. Front Cell Infect Microbiol, 2020, 10: 326. DOI: 10.3389/fcimb.2020.00326. [8] CHEN C, LU T. Development and challenges of antimicrobial peptides for tTherapeutic applications[J]. Antibiotics (Basel), 2020, 9(1):24. [9] 黄小建, 李跃龙, 戚南山,等. 抗菌肽的生物学活性及其在畜禽生产中的作用 [J]. 动物医学进展, 2020, 41(12): 115-119. [10] 李云香, 姚倩, 任玫,等. 抗菌肽作用机制研究进展 [J]. 动物医学进展, 2019, 40(9): 98-103. [11] 徐珂, 张丽萍, 张园园,等. 微生物源抗菌肽表达系统研究进展及改造策略 [J]. 河北科技大学学报, 2019, 40(5): 454-460. [12] 陈亮, 李瑞静, 黄亮,等. 微生物抗菌肽及其在动物生产中的应用[J]. 中国畜牧杂志, 2017, 53(2): 15-19. [13] PISOSCHI A, POP A, GEORGESCU C, et al. An overview of natural antimicrobials role in food[J]. Eur J Med Chem, 2018,143: 922-935.DOI:10.1016/j.ejmech.2017.11.095.

[1]	李娟娟, 史冬梅. 马拉色菌菌丝阳性毛发型扁平苔癣1例[J]. 中国真菌学杂志, 2022, 17(6): 500-502.
[2]	张华, 薛潇春, 沈玉美, 赵红信, 潘炜华. 马拉色菌在消化相关疾病中的研究进展[J]. 中国真菌学杂志, 2022, 17(6): 513-516.
[3]	郝小康, 任立汆. 马拉色菌病误诊为痤疮、湿疹2例分析[J]. 中国真菌学杂志, 2022, 17(5): 399-401.
[4]	杨虹, 高志琴, 刘思禹, 谭静文, 徐红, 陈健, 杨连娟. Microsphaeropsis arundinis引起右前臂皮肤暗色丝孢霉病1例及文献分析[J]. 中国真菌学杂志, 2022, 17(3): 220-224.
[5]	杨卓, 杨欢, 于继凯, 周云, 岳剑, 李淑霞. 真菌菌群在炎症性肠病中的作用研究进展[J]. 中国真菌学杂志, 2022, 17(3): 255-259.
[6]	曲昱颖, 史冬梅. 真菌与银屑病发生发展及治疗相关性研究进展[J]. 中国真菌学杂志, 2021, 16(6): 423-427.
[7]	林洁, 杨连娟, 杨名珍. 头颈部特应性皮炎患者面部马拉色菌的菌种分布[J]. 中国真菌学杂志, 2021, 16(3): 161-165.
[8]	张敬霞, 崔恩博, 张鞠玲, 郭莉娜, 徐英春, 曲芬. 肝病患者无菌部位酵母菌感染的临床特征及抗真菌药物的敏感性分析[J]. 中国真菌学杂志, 2020, 15(1): 15-21.
[9]	张焕梅. 卢立康唑乳膏联合柳烯酸溶液喷雾剂治疗马拉色菌毛囊炎疗效观察[J]. 中国真菌学杂志, 2020, 15(1): 52-54.
[10]	朱红梅, 梁晓博, 温海, 李斌. 中药洗液联合联苯苄唑乳膏治疗马拉色菌毛囊炎疗效观察[J]. 中国真菌学杂志, 2019, 14(5): 277-279.
[11]	孙祺琳, 王桂祯, 陈骏, 刘洋, 杨雅骊, 徐慧. 马拉色菌引起乳房垢着病1例并文献分析[J]. 中国真菌学杂志, 2019, 14(3): 164-168.
[12]	戚欣雨, 胡永轩. 马拉色菌感染的光学治疗研究进展[J]. 中国真菌学杂志, 2018, 13(5): 318-320.
[13]	郭莉娜, 徐志鹏, 王瑶, 王贺, 赵颖, 肖盟, 徐英春. 微量肉汤稀释法比较分析不同培养时间念珠菌属对唑类药物的敏感性[J]. 中国真菌学杂志, 2016, 11(6): 332-336.
[14]	孙玉鲁, 张锐利, 苏芳. 卫凤莲硝酸舍他康唑的临床应用研究进展[J]. 中国真菌学杂志, 2015, 10(4): 252-256.
[15]	唐新平, 何俐勇, 樊龙中, 仇建国. 南海海军部队特发性瘙痒症患者皮肤马拉色菌定植调查[J]. 中国真菌学杂志, 2014, 9(6): 339-341,334.