关键词: 红色毛癣菌, 基因型, TRS, 药敏试验

Abstract: Objective To study the relationship between the genotype of Trichophyton rubrum and the site of infection and drug sensitivity in the area. Methods Perform intraspecies typing based on the genotyping of the ribosomal rDNA non-transcribed region (NTS) of Trichophyton rubrum, and test the drug sensitivity according to the "CLSI-M38-A2" protocol. Results There were five banding types in 106 Trichophyton rubrum TRS-1 genotypes, including TypeⅠ52 (49.06%), TypeⅡ14 (13.21%), TypeⅢ5 (4.72%), TypeⅣ3 (2.83%), and others 32 strains (30.18%). There are three banding types in TRS-2 genotypes, including TypeⅠ68 strains (64.15%), TypeⅡ6 strains (5.66%), and others 32 strains (30.19%). The geometric mean MICs of drug sensitivity results from low to high were terbinafine (0.0092 μg/mL), voriconazole (0.0181μg/m), itraconazole (0.1491 μg/mL), ketoconazole (0.1630 μg/mL), and fluconazole (2.3164 μg/mL). 42 strains showed insensitivity to antifungal drugs, and the insensitive strains TRS-1 and TRS-2 were mainly type I. Conclusion Both the TRS-1 and TRS-2 genotypes of Trichophyton rubrum in Hainan are mainly TypeⅠ. Terbinafine (0.0092 μg/mL) and voriconazole (0.0181 μg/mL) have the lowest geometric mean MICs. This result found that the insensitive strains in this area have little relationship with the red hair genotype, and have nothing to do with the infection site, and may be related to the source.

Key words: Trichopyton rubrum, genotyping, TRS, sceptibility test