两种方法纯化临床分离阿萨希毛孢子菌荚膜多糖GXM

中国真菌学杂志　 2022, Vol. 17 　Issue (5): 359-362,366.

两种方法纯化临床分离阿萨希毛孢子菌荚膜多糖GXM

肖英伦¹, 邓光远¹, 张轩¹, 罗燕芬¹, 张伟铮²

1. 广州中医药大学第二附属医院广东省中医院检验医学部, 广州 510006;
2. 广州市干部健康管理中心广州市第十一人民医院医学检验科, 广州 510530

收稿日期:2021-12-03 发布日期:2022-10-26
通讯作者: 张伟铮,E-mail:zwzzw83@163.com E-mail:zwzzw83@163.com
作者简介:肖英伦,男(汉族),本科,初级技师.E-mail:xyl981027@126.com
基金资助:
广东省中医院院内专项青年基金（YN2019QL02）；广东省中医药局中医药科研项目基金（20212086）

Two methods for purification and clinical isolation of capsular polysaccharide GXM from Trichosporon asahii

XIAO Yinglun¹, DENG Guangyuan¹, ZHANG Xuan¹, LUO Yanfen¹, ZHANG Weizheng²

1. The Clinical Laboratory of The Second Affiliated Hospital of Guangzhou University of Chinese Medicine/Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510006, China;
2. The Clinical Laboratory of Guangzhou Cadre Health Management Center/The 11^th People's Hospital of Guangzhou, Guangzhou 510530, China

Received:2021-12-03 Published:2022-10-26

摘要/Abstract

摘要： 目的使用两种方法分离和纯化临床分离阿萨希毛孢子菌的荚膜多糖葡萄糖醛酸木糖甘露聚糖（GXM）。方法对临床分离的阿萨希毛孢子菌进行增菌，采用无水乙醇沉淀荚膜多糖，通过十六烷基三甲基溴化铵（CTAB）对GXM特异性沉淀，分别采用透析法和萃取法分离GXM-CTAB复合物从而纯化GXM。使用苯酚硫酸法测定GXM的浓度。结果通过透析法从200 mL培养上清中获得了约6.6 mg的GXM，阿萨希毛孢子菌GXM的获得率（GXM/GXM和GalXM混合多糖）为13.9%（6.6/47.4）。通过萃取法从200 mL培养上清中获得了约8.4 mg的GXM，阿萨希毛孢子菌GXM的获得率为14.2%（8.4/59）。结论两种方法都成功地提取和纯化了阿萨希毛孢子菌荚膜多糖GXM，萃取法较透析法更为高效。

关键词: 阿萨希毛孢子菌, 荚膜多糖, GXM

Abstract: Objective Isolation and purification of xylomannan glucuronate (gxm) from the capsular polysaccharide of Trichosporon asahii in two methods. Methods The clinical isolates of Trichosporon asahii were inoculated, the capsular polysaccharide was precipitated by anhydrous ethanol, GXM was precipitated by cetyltrimethyl ammonium bromide (CTAB), and GXM was purified by dialysis and extraction, respectively. The concentration of GXM was determined by phenol sulfuric acid method. Results about 6.6mg GXM was obtained from 200 mL culture supernatant by dialysis, and the yield of GXM (GXM/GXM and GalXM mixed polysaccharide) was 13.9% (6.6/47.4). About 8.4mg GXM was obtained from 200 mL culture supernatant by extraction method, and the GXM yield of T. assahi was 14.2% (8.4/59). Conclusion These two methods had successfully extracted and purified the capsular polysaccharide GXM from T. assahi, and the extraction method was more efficient than dialysis method.

Key words: Trichosporon asahii, capsularpolysaccharide, GXM

中图分类号:

R379.9A

肖英伦, 邓光远, 张轩, 罗燕芬, 张伟铮. 两种方法纯化临床分离阿萨希毛孢子菌荚膜多糖GXM[J]. 中国真菌学杂志, 2022, 17(5): 359-362,366.

XIAO Yinglun, DENG Guangyuan, ZHANG Xuan, LUO Yanfen, ZHANG Weizheng. Two methods for purification and clinical isolation of capsular polysaccharide GXM from Trichosporon asahii[J]. Chinese Journal of Mycology, 2022, 17(5): 359-362,366.

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