烟曲霉cyp51A基因序列BLAST数据库的建立

中国真菌学杂志　 2022, Vol. 17 　Issue (5): 353-358.

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烟曲霉cyp51A基因序列BLAST数据库的建立

洪翩翩¹, 陈玉萍¹, 郑楠², 刘沐桑¹

1. 中国医学科学院北京协和医学院皮肤病研究所, 南京 210042;
2. 南京大学医学院, 南京 210093

收稿日期:2022-01-24 发布日期:2022-10-26
通讯作者: 刘沐桑,E-mail:liums@pumcderm.cams.cn E-mail:liums@pumcderm.cams.cn
作者简介:洪翩翩,女(汉族),硕士研究生在读.E-mail:pyhongpianpian@student.pumc.edu.cn
基金资助:
国家自然科学基金青年基金（81501726）；江苏省自然科学基金青年基金（BK20150069）；中国医学科学院中央级公益性科研院所基本科研业务费临床与转化医学研究基金项目（2019XK320077）

Construction of BLAST database of Aspergillus fumigatus cyp51A gene sequence

HONG Pianpian¹, CHEN Yuping¹, ZHENG Nan², LIU Musang¹

1. Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China;
2. Medical School of Nanjing University, Nanjing 210093, China

Received:2022-01-24 Published:2022-10-26

摘要/Abstract

摘要： 目的构建我国烟曲霉cyp51A基因序列BLAST数据库。方法对本课题组收集的143株耐药与非耐药临床烟曲霉进行cyp51A基因测序，使用BLAST工具包汇总测序结果，构建本地数据库。结果此BLAST数据库包含143株耐药与非耐药烟曲霉cyp51A基因序列，以1株耐药与1株非耐药待测烟曲霉标本的cyp51A序列对此数据库进行检索和比对可以确定烟曲霉cyp51A基因型。结论本数据库有望用于cyp51A基因的检索与比对，鉴定烟曲霉cyp51A耐药基因型。

关键词: 烟曲霉, cyp51A, 唑类耐药, BLAST检索, 数据库

Abstract: Objective To construct BLAST database of Aspergillus fumigatus cyp51A gene sequence. Methods The cyp51A genes of the 143 drug-resistant and non-drug-resistant clinical Aspergillus fumigatus strains collected by our group were sequenced, and the sequencing results were put together to construct a local BLAST database. Results This BLAST database contains cyp51A gene sequences of 143 drug-resistant and non-drug-resistant Aspergillus fumigatus strains. cyp51A sequences of Aspergillus fumigatus specimens can be confirmed by searching this database. Conclusion This BLAST database can be used for search and comparison of cyp51A gene to identify cyp51A drug-resistant genotypes of Aspergillus fumigatus.

Key words: Aspergillus fumigatus, cyp51A, azole-resistance, BLAST search, database

中图分类号:

R379.6A

洪翩翩, 陈玉萍, 郑楠, 刘沐桑. 烟曲霉cyp51A基因序列BLAST数据库的建立[J]. 中国真菌学杂志, 2022, 17(5): 353-358.

HONG Pianpian, CHEN Yuping, ZHENG Nan, LIU Musang. Construction of BLAST database of Aspergillus fumigatus cyp51A gene sequence[J]. Chinese Journal of Mycology, 2022, 17(5): 353-358.

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烟曲霉cyp51A基因序列BLAST数据库的建立

Construction of BLAST database of Aspergillus fumigatus cyp51A gene sequence

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