CHK1基因对白念珠菌体外超微结构及氟康唑最低抑菌浓度的影响

中国真菌学杂志　 2022, Vol. 17 　Issue (4): 265-268,288.

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CHK1基因对白念珠菌体外超微结构及氟康唑最低抑菌浓度的影响

黄悦¹, 蔡良奇¹, 张子平², 程波²

1. 厦门大学附属第一医院皮肤科, 厦门大学医学院, 福建医科大学第三临床医学院, 厦门 361002;
2. 福建医科大学附属第一医院皮肤科, 福州 350004

收稿日期:2021-09-07 出版日期:2022-08-28 发布日期:2022-08-31
通讯作者: 程波,E-mail:chengbo630415@126.com E-mail:chengbo630415@126.com
作者简介:黄悦,女(汉族),博士,副主任医师.E-mail:huangyueyuran@163.com

Influence of CHK1 gene on ultrastrcture and fIuconazole susceptibility of Candida albicans

HUANG Yue¹, CAI Liangqi¹, ZHANG Ziping², CHENG Bo²

1. Department of Dermatology, the First Affiliated Hospital, Xiamen University, School of Medicine, The Third Clinical Medical College, Fujian Medical University, Xiamen 361002, China;
2. Department of Dermatology, the First Affiliated Hospital, Fujian Medical University, Fuzhou 350005, China

Received:2021-09-07 Online:2022-08-28 Published:2022-08-31

摘要/Abstract

摘要： 目的探讨CHK1基因对白念珠菌体外超微结构及对氟康唑最低抑菌浓度的影响。方法设置实验组CHK21组（chk/chk双等位基因缺陷株），对照组标准株组（CAF-2）。通过扫描、透射电镜观察两组菌株超微结构的变化，并采用NCCLS-M27A方案对白念珠菌标准株CAF-2、CHK1基因缺陷株进行氟康唑体外药敏试验，观察其对氟康唑最低抑菌浓度的影响。结果透射电镜下标准株组细胞壁各层结构完整；CHK1基因缺陷株细胞壁的电子致密层明显变薄，部分区域出现局灶性缺失的现象。扫描电镜下标准株酵母细胞表面光滑，出芽数目较多；CHK1基因缺陷株细胞表面粗糙，可见小斑块或者斑片状凸起。氟康唑对白念珠菌标准株CAF-2、CHK1缺陷株CHK21的MIC值分别为1 μg/mL、0.125~0.25 μg/mL。结论 CHK1基因可能调控白念珠菌细胞壁的生物合成以及对氟康唑的敏感性。

关键词: 白念珠菌, 氟康唑, 缺陷株, 药敏试验, 超微结构

Abstract: Objective To explore the influence of CHK1 gene on ultrastructure of Candida albicans and the susceptibility with fIuconazole. Methods Ultrastructure of Candida albicans in CHK21 group(chk1/chk1 double mutant) and CAF-2 group were observed under scanning electronmicroscope(SEM) and transmission electron microscope(TEM). We also compare the susceptibility with fluconazole of two strains. Result Under scanning electron microscope, two groups of cells appeared round or ellipse with the CHK21 showed rough and uneven surface while CAF-2 cells appeared smooth with much more budding scars. Under transmission electron microscope,the walls of CHK21 showed the electron dense layers disappeared somewhere. In contrast CAF-2 cells wall were integrity. The MIC of CAF-2 and CHK21 with fluconazole were 1ug/mL、0.125~0.25ug/mL respectly. Conclusion CHK1 gene may regulate the biosynthesis of cell wall in Candida albicans and they may also play a role in the susceptibility of C.albicans with fiuconazole.

Key words: Candida albicans, fluconazole, mutant strain, antifugal susceptibility test, ultrastructure

中图分类号:

R379.4

黄悦, 蔡良奇, 张子平, 程波. CHK1基因对白念珠菌体外超微结构及氟康唑最低抑菌浓度的影响[J]. 中国真菌学杂志, 2022, 17(4): 265-268,288.

HUANG Yue, CAI Liangqi, ZHANG Ziping, CHENG Bo. Influence of CHK1 gene on ultrastrcture and fIuconazole susceptibility of Candida albicans[J]. Chinese Journal of Mycology, 2022, 17(4): 265-268,288.

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CHK1基因对白念珠菌体外超微结构及氟康唑最低抑菌浓度的影响

Influence of CHK1 gene on ultrastrcture and fIuconazole susceptibility of Candida albicans

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