微进化对阿萨希毛孢子菌感染宿主的影响

中国真菌学杂志　 2022, Vol. 17 　Issue (3): 213-219.

微进化对阿萨希毛孢子菌感染宿主的影响

彭卓颖, 廖勇, 巴根, 杨鑫, 张骞宇, 杨蓉娅

中国人民解放军总医院第七医学中心皮肤科, 北京 100700

收稿日期:2020-04-26 出版日期:2022-06-28 发布日期:2022-07-02
通讯作者: 杨蓉娅,E-mail:yangrya@sina.com E-mail:yangrya@sina.com
作者简介:彭卓颖,女(汉族),硕士,住院医师.E-mail:zofiapeng@126.com
基金资助:
国家自然科学基金(81571972);北京市科技新星项目(Z161100004916139)

The effects of microevolution on Trichosporon assahii infection

PENG Zhuoying, LIAO Yong, BA Gen, YANG Xin, ZHANG Qianyu, YANG Rongya

Department of Dermatology, The Seventh Medical Center of PLA General Hospital, Beijing 100700, China

Received:2020-04-26 Online:2022-06-28 Published:2022-07-02

摘要/Abstract

摘要： 目的研究微进化对阿萨希毛孢子菌(Trichosporon asahii,T.asahii)感染宿主情况及感染后机体免疫反应等方面的影响。方法用微进化株(TEVO)与阿萨希毛孢子菌原代株(TO)感染Balb/c小鼠,比较两株菌感染小鼠脏器组织中真菌的相对载量、髓过氧化物酶(myeloperoxidase,MPO)、血清β-葡聚糖水平以及脾脏T细胞分化差异。结果 TEVO菌株以孢子状态为主,TO菌株以菌丝生长状态为主;TEVO组小鼠各组织相对载量、血浆(1,3)-β-D-葡聚糖水平及MPO浓度均明显低于TO组;TEVO组小鼠脾脏中CD4⁺T细胞和CD8⁺T细胞的比例均高于TO组。结论微进化后的TEVO菌株对宿主的感染能力以及感染前期宿主对TEVO菌株的防御和杀伤能力均低于TO菌株,这可能与TEVO菌株表型变化密切相关。

关键词: 微进化, 阿萨希毛孢子菌, 表型

Abstract: Objective To study the effects of microevolution on T. asahii infecting host and the immune response of the body. Methods Use the evolutional strain (TEVO) and original strain (TO) to infect the Balb/c mice, and comparing the differences of fungal relative load and MPO in the tissues、β-glucan in the serum and T cell differentiation in spleens. Results TEVO stains were almost spores, and TO stains were mycelia in morphology. The fungal relative load, MPO and β-glucan concentration of the TEVO group were significantly lower than those of the TO group. The CD4⁺T cells and CD8⁺T cells in the spleens of the TEVO group were more than that in the TO group. Conclusion The ability of the evolutional strain infecting host and the host defense to the evolutional strain in the early stage of infection were all lower than those of original strain, which may be closely related to the phenotype change of the evolutional strain.

Key words: microevolution, Trichosporium asahi, phenotype

中图分类号:

R379.9

彭卓颖, 廖勇, 巴根, 杨鑫, 张骞宇, 杨蓉娅. 微进化对阿萨希毛孢子菌感染宿主的影响[J]. 中国真菌学杂志, 2022, 17(3): 213-219.

PENG Zhuoying, LIAO Yong, BA Gen, YANG Xin, ZHANG Qianyu, YANG Rongya. The effects of microevolution on Trichosporon assahii infection[J]. Chinese Journal of Mycology, 2022, 17(3): 213-219.

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