贝莱斯芽孢杆菌Y6抗菌肽抑制白念珠菌活性的研究

中国真菌学杂志　 2022, Vol. 17 　Issue (3): 183-187,194.

贝莱斯芽孢杆菌Y6抗菌肽抑制白念珠菌活性的研究

杨成^1,2, 乔云龙³, 厉荣玉¹, 熊延靖¹, 汤兴丽¹

1. 皖南医学院微生物学与免疫学教研室, 芜湖 241002;
2. 活性生物大分子研究安徽省重点实验室(皖南医学院), 芜湖 241002;
3. 皖南医学院药学院, 芜湖 241002

收稿日期:2021-07-08 出版日期:2022-06-28 发布日期:2022-07-02
作者简介:杨成,男(汉族),硕士,副教授.E-mail:58910583@qq.com
基金资助:
安徽省重点实验室自主课题项目(LAB202002);安徽省大学生创新创业训练项目(S202010368061)

Antifungal activity of Bacillus velezensis Y6 peptide against Candida albicans

YANG Cheng^1,2, QIAO Yunlong³, LI Rongyu¹, XIONG Yanjing¹, TANG Xingli¹

1. Department of Microbiology and Immunology, Wannan Medical College, Wuhu 241002, China;
2. Anhui Province Key Laboratory of Biological Macro-molecules Research (Wannan Medical College), Wuhu 241002, China;
3. School of Pharmacy, Wannan Medical College, Wuhu 241002, China

Received:2021-07-08 Online:2022-06-28 Published:2022-07-02

摘要/Abstract

摘要： 目的对贝莱斯芽孢杆菌Y6代谢物抑制白念珠菌活性及其作用机理进行初步研究。方法采用平板结合牛津杯研究贝莱斯芽孢杆菌Y6发酵上清液对白念珠菌抑制活性,梯度稀释法测定了最小抑菌浓度(MIC),通过薄层色谱法对代谢物的活性成分进行筛检;同时比较抗菌肽处理前和处理后菌体胞内大分子释放量及荧光染料PI的摄入量的变化,考察抗菌肽对胞膜通透性影响。结果贝莱斯芽孢杆菌Y6代谢物能有效抑制白念珠菌生长繁殖,测试浓度为10 mg·mL^-1时,抑菌圈直径达到(38.2±0.5) mm,其MIC浓度为0.625 mg·mL^-1;通过薄层色谱结合茚三酮显色对活性组分筛检发现Y6代谢物中有2个抗菌组分,其中主要活性组分遇茚三酮显紫色,推测为肽类组分;经抗菌肽处理后的白念珠菌胞内大分子释放量及PI摄入量明显高于对照组,表明菌株Y6抗菌肽对白念珠菌胞膜完整性具有一定的损伤作用。结论贝莱斯芽孢杆菌Y6产肽类抗菌物质,且对白念珠菌细胞膜存在一定的损伤作用,从而产生明显的抗菌活性。

关键词: 贝莱斯芽孢杆菌, 白念珠菌, 抗菌肽, 抗菌活性

Abstract: Objective To study the inhibitory activity of Bacillus velezensis Y6 metabolite against C. albicans and its mechanism. Methods The inhibitory activity of Bacillus velezensis Y6 fermentation supernatant against C. albicans was studied by plate combined with Oxford cup. The minimum inhibitory concentration (MIC) was determined by gradient dilution method. The active components of the metabolites were screened by TLC. At the same time, the changes in the amount of intracellular macromolecules released and the intake of fluorescent dye PI before and after the treatment of antimicrobial peptides were compared to investigate the effect of antimicrobial peptides on membrane permeability. Results Bacillus velezensis Y6 metabolite could effectively inhibit the growth of C.albicans. When the test concentration was 10 mg·mL^-1, the inhibition zone diameter reached (38.2±0.5) mm, and the MIC concentration was 0.625 mg·mL^-1. Two antimicrobial components were found in the metabolites of Y6 by TLC. The main active component appears purple when encountering ninhydrin,so it may be peptide. The release of intracellular macromolecules and the intake of PI in C. albicans treated with antimicrobial peptides were significantly higher than those in the control group, indicating that the antimicrobial peptides of strain Y6 had a certain damage effect on the integrity of the membrane of C. albicans. Conclusion Bacillus velezensis Y6 produces peptide antifungal substances, which can damage the cell membrane of C.albicans, resulting in obvious antimicrobial activity.

Key words: Bacillus velezensis, Candida albicans, antifungal peptide, antimicrobial activity

中图分类号:

R379.4

杨成, 乔云龙, 厉荣玉, 熊延靖, 汤兴丽. 贝莱斯芽孢杆菌Y6抗菌肽抑制白念珠菌活性的研究[J]. 中国真菌学杂志, 2022, 17(3): 183-187,194.

YANG Cheng, QIAO Yunlong, LI Rongyu, XIONG Yanjing, TANG Xingli. Antifungal activity of Bacillus velezensis Y6 peptide against Candida albicans[J]. Chinese Journal of Mycology, 2022, 17(3): 183-187,194.

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