肉桂醛对阿萨希毛孢子菌临床分离株的生长及其生物膜形成的影响

中国真菌学杂志　 2021, Vol. 16 　Issue (5): 296-302.

肉桂醛对阿萨希毛孢子菌临床分离株的生长及其生物膜形成的影响

张伟铮¹, 肖英伦¹, 彭湘明², 李松¹, 张轩¹, 陈茶¹

1. 广州中医药大学第二附属医院/广东省中医院检验医学部, 广州 510006;
2. 广州市红十字会医院检验科, 广州 510220

收稿日期:2020-05-19 发布日期:2021-10-28
通讯作者: 陈茶,E-mail:chencha906@163.com E-mail:chencha906@163.com
作者简介:张伟铮,男(汉族),硕士,副主任技师.E-mail:zwzzw83@163.com
基金资助:
广东省中医药局中医药科研项目基金（20212086）

The effect of cinnamaldehyde on the growth and biofilm formation of Trichosporon asahii

ZHANG Weizheng¹, XIAO Yinglun¹, PENG Xiangming², LI Song¹, ZHANG Xuan¹, CHEN Cha¹

1. The Clinical Laboratory of The Second Affiliated Hospital of Guangzhou University of Chinese Medicine/Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510006, China;
2. The Clinical Laboratory of The Guangzhou Red Cross Hospital, Guangzhou 510220, China

Received:2020-05-19 Published:2021-10-28

摘要/Abstract

摘要： 目的研究中药单体肉桂醛是否对阿萨希毛孢子菌的生长及其生物膜形成有影响。方法选取临床分离菌株进行ITS基因序列分析及VITEK MS鉴定，通过培养建立体外生物膜模型。倍比稀释法测定1050 mg/L、525 mg/L、262.5 mg/L和131.25 mg/L浓度肉桂醛溶液对阿萨希毛孢子菌生长的抑制作用，银染法观察不同浓度肉桂醛对阿萨希毛孢子菌生物膜形成的影响。结果实验中各浓度的肉桂醛都能抑制阿萨希毛孢子菌的生长，延长生物膜的形成时间，且肉桂醛浓度越高，其作用效果越明显。当肉桂醛浓度达到1050 mg/L时，对阿萨希毛孢子菌生长的抑制效果最显著，该浓度下培养基孔内未见阿萨希毛孢子菌生长。银染法观察可见，此浓度下培养基内未形成生物膜，表明1050 mg/L浓度肉桂醛对阿萨希毛孢子菌生物膜的形成产生了明显的抑制作用。结论肉桂醛能够抑制阿萨希毛孢子菌生长及其生物膜形成。

关键词: 肉桂醛, 阿萨希毛孢子菌, 生物膜

Abstract: Objective To find the effect of cinnamaldehyde on the growth and biofilm formation of Trichosporon asahii(T. asahii). Methods Clinical isolates were selected for gene sequencing and VITEK MS identification, and the biofilm model was established in vitro. The inhibitory effects of cinnamaldehyde at 1050 mg/L, 525 mg/L, 262.5 mg/L and 131.25 mg/L on the growth of T. asahii were determined by multiple dilution method.The effects of different concentrations of cinnamaldehyde on the biofilm formation of T. asahii. were observed by silver staining method. Results Each concentration of cinnamaldehyde could affect the growth of T. asahii and prolong the formation time of biofilm. The higher the concentration of cinnamaldehyde, the more obvious the effect was. When the concentration of cinnamaldehyde reached 1050 mg/L, the inhibition effect of cinnamaldehyde on the growth of T. asahii was the most significant, the growth of T. asahii was not observed in the medium wells at the concentration. Silver staining showed that no biofilm was formed in the medium at this concentration, indicating that the concentration of cinnamaldehyde at 1050mg/L had a significant inhibitory effect on the formation of biofilm of T. asahii. Conclusion Cinnamaldehyde can affect the growth and biofilm formation of T. asahii.

Key words: cinnamaldehyde, Trichosporon asahii, biofilm

中图分类号:

R379.9

张伟铮, 肖英伦, 彭湘明, 李松, 张轩, 陈茶. 肉桂醛对阿萨希毛孢子菌临床分离株的生长及其生物膜形成的影响[J]. 中国真菌学杂志, 2021, 16(5): 296-302.

ZHANG Weizheng, XIAO Yinglun, PENG Xiangming, LI Song, ZHANG Xuan, CHEN Cha. The effect of cinnamaldehyde on the growth and biofilm formation of Trichosporon asahii[J]. Chinese Journal of Mycology, 2021, 16(5): 296-302.

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