头颈部特应性皮炎患者面部马拉色菌的菌种分布

中国真菌学杂志　 2021, Vol. 16 　Issue (3): 161-165.

头颈部特应性皮炎患者面部马拉色菌的菌种分布

林洁¹, 杨连娟², 杨名珍³

1. 上海市宝山区仁和医院皮肤科, 上海 200431;
2. 上海同济大学医学院附属上海市皮肤病医院皮肤科, 上海 200050;
3. 宁夏回族自治区银川市贺兰县人民医院药剂科, 宁夏 750200

收稿日期:2021-03-04 出版日期:2021-06-28 发布日期:2021-06-28
通讯作者: 杨连娟,E-mail:zhy52808290@qq.com E-mail:zhy52808290@qq.com
作者简介:林洁,女(汉族),本科,主治医师.E-mail:lj@193idc.com
基金资助:
上海市宝山区科学技术委员会科技创新专项资金项目（20-E-27）

Distribution of Malassezia species on the face of patients with atopic dermatitis of head and neck

LIN Jie¹, YANG Lianjuan², YANG Mingzhen³

1. Department of Dermatology, Renhe Hospital, Baoshan District, Shanghai 200431, China;
2. Department of Dermatology, Shanghai Skin Disease Hospital, School of Medicine of Tongji University, Shanghai 200050, China;
3. Department of Pharmacy, The People's Hospital of Helan County, Ningxia 750200, China

Received:2021-03-04 Online:2021-06-28 Published:2021-06-28

摘要/Abstract

摘要： 目的探讨马拉色菌（Malassezia）在头颈部特应性皮炎（atopic dermatitis，AD）患者中面部菌种分布以及与严重程度之间的关系。方法 2020年9月—12月共招募45例AD患者和50名健康志愿者，无菌棉签对患者和志愿者面部皮损区和非皮损区分别进行皮肤鳞屑取样，用荧光实时定量PCR的方法检测两组人群的7种马拉色菌的拷贝数，进行菌种分布以及数量的横断面分析。结果头颈部AD患者面部皮损区限制马拉色菌和球形马拉色菌的拷贝数分别为健康志愿者的3.13倍（P<0.05）和6.69倍（P<0.05），两者皆与患者AD病情严重度相关（P<0.05）。结论头颈部AD患者面部皮损区限制马拉色菌和球形马拉色菌含量均高于健康人，两者含量均与AD患者病情严重程度相关。

关键词: 马拉色菌, 特应性皮炎, 实时荧光定量PCR, 皮肤, 定植

Abstract: Objective To investigate the distribution of Malassezia in patients with head and neck atopic dermatitis(AD) and its relationship with severity. Methods From September to December, 2020, 45 AD patients and 50 healthy volunteers were recruited. Scale samples were taken from the lesion and non-lesion areas on the face both of patients and volunteers with sterile cotton swabs, and the number of copies of 7 species of Malassezia were detected by quantitative real-time PCR. The species distribution and number were analyzed by using cross-sectional analysis. Results For head and neck AD patients,the Malassezia restricta and Malassezia globosa in the facial lesions were 3.13 times (P<0.05) and 6.69 times (P<0.05) of the healthy volunteers, respectively. Both of them were correlated with the severity of AD (P<0.05). Conclusion Malassezia restricta and Malassezia globosa of lesion areas on the face were more in head and neck AD patients than that in healthy people, and their levels were correlated with the severity of the disease.

Key words: Malassezia, atopic dermatitis, quantitative real-time PCR, skin, colonization

中图分类号:

R379.9

林洁, 杨连娟, 杨名珍. 头颈部特应性皮炎患者面部马拉色菌的菌种分布[J]. 中国真菌学杂志, 2021, 16(3): 161-165.

LIN Jie, YANG Lianjuan, YANG Mingzhen. Distribution of Malassezia species on the face of patients with atopic dermatitis of head and neck[J]. Chinese Journal of Mycology, 2021, 16(3): 161-165.

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