分离自1例慢性阻塞性肺疾病患者的Aspergillus lentulus在蜡螟模型上的毒力初探

摘要/Abstract

摘要： 目的建立Aspergillus lentulus（A. lentulus或A.L）感染动物模型，借动物模型初步探究A. lentulus的毒力。方法将125只蜡螟随机分成5组，以Aspergillus lentulus临床株、Aspergillus lentulus标准株作为实验组，烟曲霉、白念珠菌为对照组，PBS为空白对照组。实验组及对照组菌株分别制成10⁶ CFU/mL孢子悬液，感染各组蜡螟。记录72 h内蜡螟的生存情况并制作生存曲线，24 h后提取蜡螟肠道组织，HE染色组织切片观察肠道组织损伤情况，用组织匀浆法，测定蜡螟肠道内真菌载量及真菌逆培养阳性率，用真菌荧光染色法观察肠道培养真菌镜下形态。结果 A. lentulus临床株和A. lentulus标准株的蜡螟存活数与对照组之间差异有统计学意义（P<0.05）；结果显示A. lentulus临床株和A. lentulus标准株肠壁结构大致正常，局部可见水肿少量菌丝、孢子及炎症细胞浸润，对照组肠道结构破坏严重，可见菌丝、孢子及大量炎症细胞浸润；不同菌种感染蜡螟幼虫各组肠道载菌量及真菌逆培养阳性率比较，差异具有统计学意义（P<0.05）；真菌荧光显微镜观察，A. lentulus临床株和标准株菌丝多、孢子少，白念珠菌和烟曲霉组孢子多。结论与烟曲霉和白念珠菌相比，A. lentulus菌株对蜡螟毒力和肠道损伤能力较弱且致死率低。

关键词: Aspergillus lentulus, 蜡螟, 烟曲霉, 白念珠菌, 毒力

Abstract: Objective Establish an animal model of Aspergillus lentulus (A. lentulus or A.L) infection, and use the animal model to explore the virulence of A. lentulus. Methods One hundred and twenty-five Galleria mellonella were randomly divided into five groups, with Aspergillus lentulus clinical strain and Aspergillus lentulus standard strain as the experimental group, Aspergillus fumigatus and Candida albicans as the control group, and PBS as the blank control group. The experimental group and the control group were made into 10⁶ CFU/mL spore suspension, respectively, and infected with each group of Galleria mellonella. The survival of Galleria mellonella within 72 hours was recorded and the survival curve was made. The intestinal tissues of Galleria mellonella were extracted 24 hours later. The intestinal tissue damage was observed by HE staining. The fungal load in the intestinal tract of the Galleria mellonella and the positive rate of fungal reverse culture were determined by tissue homogenization. The fungal morphology of the intestinal culture was observed by fungal fluorescence staining. Results There was a statistical difference between the survival numbers of Galleria mellonellas of A. lentulus clinical strain and A. lentulus standard strain and A. fumigatus group and C. albicans group (P<0.05); The results showed that the intestinal wall structure of the clinical strain of A. lentulus and the standard strain of A. lentulus was roughly normal, with a small amount of hyphae, spores and inflammatory cell infiltration in local edema. The intestinal structure of the control group was seriously damaged, with hyphae, spores and a large number of inflammatory cell infiltrations; There was a statistical difference in the intestinal fungal load and the positive rate of fungal reverse culture in each group of different fungal species infected Galleria mellonellas (P<0.05); According to the fluorescence microscope observation of the fungus, the clinical strain and standard strain of A. lentulus had more hyphae and fewer spores, while the C. albicans, and A. fumigatus group had more spores. Conclusion Compared with A.fumigatus and C. albicans, the A. lentulus strain has weaker virulence and intestinal damage to larvaes and has a lower lethality rate.

Key words: A. lentulus, Galleria mellonella, A. fumigatus, C. albicans, virulence

中图分类号:

R379.6

谢韵, 张丽娟, 帕丽达·阿布利孜. 分离自1例慢性阻塞性肺疾病患者的Aspergillus lentulus在蜡螟模型上的毒力初探[J]. 中国真菌学杂志, 2021, 16(3): 155-160.

XIE Yun, ZHANG Lijuan, PALIDAE·Abliz. A preliminary study on the virulence of Aspergillus lentulus isolated from a patient with COPD on the Galleria mellonella model[J]. Chinese Journal of Mycology, 2021, 16(3): 155-160.

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