硬脂酸单甘油酯协同卡泊芬净的体外抗念珠菌活性研究

中国真菌学杂志　 2021, Vol. 16 　Issue (3): 145-149,165.

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硬脂酸单甘油酯协同卡泊芬净的体外抗念珠菌活性研究

邱丽娟¹, 巢雯¹, 石安喆², 阎澜², 吕权真², 姜远英²

1. 海军军医大学基础医学院, 上海 200433;
2. 海军军医大学药学院, 上海 200433

收稿日期:2020-03-03 出版日期:2021-06-28 发布日期:2021-06-28
通讯作者: 吕权真,E-mail:lvquanzhen2011@163.com;姜远英,E-mail:13761571578@163.com E-mail:lvquanzhen2011@163.com;13761571578@163.com
作者简介:邱丽娟,女(汉族),硕士,实验师.E-mail:253867468@qq.com
基金资助:
上海市青年科技英才扬帆计划资助（19YF1458800）

Study on the synergistic antifungal activity of glyceryl monostearate and caspofungin in vitro

QIU Lijuan¹, CHAO Wen¹, SHI Anzhe², YAN Lan², LYU Quanzhen², JIANG Yuanying²

1. Basic Medicine School of Naval Medical University, Shanghai 200433, China;
2. School of Pharmacy of Naval Medical University, Shanghai 200433, China

Received:2020-03-03 Online:2021-06-28 Published:2021-06-28

摘要/Abstract

摘要： 目的通过化合物体外筛选，获得与临床常用抗真菌药物卡泊芬净具有协同作用的化合物，增强卡泊芬净的抗菌效果。方法采用棋盘式微量液基稀释法考察硬脂酸单甘油酯（glyceryl monostearate， GMS）与卡泊芬净（CAS）、氟康唑（FCZ）对多种念珠菌的最低抑菌浓度（MIC）和协同指数（FICI）；考察硬脂酸单甘油酯与卡泊芬净单用及联合使用时，在RPMI 1640和Spider培养基中抑制白念珠菌菌丝形成的抑菌浓度。结果在考察的绝大多数致病念珠菌中，硬脂酸单甘油酯与卡泊芬净具有协同抗菌作用，协同指数FICI≤0.5；联用硬脂酸单甘油酯后，卡泊芬净抑制菌丝形成的浓度降低。结论硬脂酸单甘油酯可以增强卡泊芬净的体外抗真菌效果，二者具有较强的协同抗念珠菌活性。

关键词: 念珠菌, 卡泊芬净, 硬脂酸单甘油酯, 协同作用

Abstract: Objective In order to improve the antifungal effect of caspofungin, we screened the synergistic effects of many compounds with caspofungin in vitro. Methods The minimum inhibitory concentration (MIC) and synergistic index (FICI) of glyceryl monostearate (GMS), caspofungin (CAS) and fluconazole (FCZ) against Candida were measured by a microdilution method. In addition, the minimum concentration of glyceryl monostearate and caspofungin used alone or in combination to inhibit the hyphae formation of Candida albicans was investigated in the RPMI 1640 and Spider medium. Results The combination of glyceryl monostearate and caspofungin showed synergistic antifungal effect on pathogenic Candida, and the synergistic index was less than 0.5. When combined with glyceryl monostearate, the concentration of caspofungin inhibiting the formation of hyphae decreased significantly. Conclusion Glyceryl monostearate could enhance the antifungal effect of caspofungin in vitro.

Key words: Candida, caspofungin, glyceryl monostearate, synergistic effect

中图分类号:

R978.5

邱丽娟, 巢雯, 石安喆, 阎澜, 吕权真, 姜远英. 硬脂酸单甘油酯协同卡泊芬净的体外抗念珠菌活性研究[J]. 中国真菌学杂志, 2021, 16(3): 145-149,165.

QIU Lijuan, CHAO Wen, SHI Anzhe, YAN Lan, LYU Quanzhen, JIANG Yuanying. Study on the synergistic antifungal activity of glyceryl monostearate and caspofungin in vitro[J]. Chinese Journal of Mycology, 2021, 16(3): 145-149,165.

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硬脂酸单甘油酯协同卡泊芬净的体外抗念珠菌活性研究

Study on the synergistic antifungal activity of glyceryl monostearate and caspofungin in vitro

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