微进化对阿萨希毛孢子菌与小鼠巨噬细胞RAW264.7相互作用的影响

摘要/Abstract

摘要： 目的研究微进化对阿萨希毛孢子菌（T.asahii）与巨噬细胞RAW264.7相互作用的影响。方法将微进化前后的T.asahii与RAW264.7细胞共培养，检测RAW264.7对两株菌的吞噬和杀伤能力以及两株菌对RAW264.7产生的细胞毒性的差异，同时分析RAW264.7自身细胞因子分泌情况的变化。结果巨噬细胞对原代菌株（TO）的吞噬能力以及杀伤率均明显高于微进化株（TEVO）；TO菌株对巨噬细胞的细胞毒性要强于TEVO；当巨噬细胞与菌株按1：3或1：6共培养24 h时，与TO共培养的巨噬细胞TNF-α和IL-6的分泌量要高于TEVO组，而按1：9共培养时TEVO组细胞因子的分泌量却高于TO组。结论微进化后的TEVO菌株与巨噬细胞的相互作用明显弱于原代株TO，这也为TEVO菌株在宿主体内长期共存提供了良好的基础。

关键词: 微进化, 阿萨希毛孢子菌, 天然免疫

Abstract: Objective To study the effect of microevolution on the interaction between T.asahii and mice macrophage RAW264.7. Methods The original strain (TO) and the evolutional strain (TEVO) were co-cultured with RAW264.7 respectively, then detecting the differences of RAW264.7 phagocytosis and killing the two strains, and the cytotoxicity of the two strains on RAW264.7; analyzing the changes of the cytokines which were secreted by RAW264.7. Results The phagocytic capacity and killing rate of RAW264.7 to TO were significantly higher than that of TEVO. TO had stronger cytotoxicity to RAW264.7 than TEVO. When RAW264.7 and strains were co-cultured with 1:3 or 1:6 for 24h, the secretion of TNF-α and IL-6 by macrophages which co-cultured with TO were higher than that of TEVO, while the secretion of cytokines in TEVO group were higher than that of TO group when co-cultured at 1:9. Conclusion The interaction between the evolutional strain with macrophage was significantly weaker than the original strain which provides a good basis for the long-term coexistence of the evolutional strain in the host.

Key words: microevolution, Trichosporon asahii, innate immunity

中图分类号:

R379.9

彭卓颖, 廖勇, 杨鑫, 张骞宇, 杨蓉娅. 微进化对阿萨希毛孢子菌与小鼠巨噬细胞RAW264.7相互作用的影响[J]. 中国真菌学杂志, 2021, 16(2): 96-100.

PENG Zhuoying, LIAO Yong, YANG Xin, ZHANG Qianyu, YANG Rongya. The effect of microevolution on the interaction between Trichosporon assahii and mice macrophage RAW264.7[J]. Chinese Journal of Mycology, 2021, 16(2): 96-100.

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