中国广西地区格特隐球菌菌种复合体的基因型分析

中国真菌学杂志　 2021, Vol. 16 　Issue (2): 84-89.

中国广西地区格特隐球菌菌种复合体的基因型分析

李炳坤¹, 黄春阳¹, 李秀楹¹, 郑艳青², 潘开素¹, 廖万清³, 曹存巍¹

1. 广西医科大学第一附属医院皮肤性病科, 南宁 530021;
2. 南宁市第四人民医院, 南宁 530023;
3. 上海长征医院皮肤性病与真菌病研究所, 上海 200003

收稿日期:2020-11-23 发布日期:2021-04-25
通讯作者: 曹存巍,E-mail:caocunwei@yeah.net E-mail:caocunwei@yeah.net
作者简介:李炳坤,男(汉族),硕士研究生在读.E-mail:992432097@qq.com
基金资助:
国家自然基金（81960567）；广西自然基金（2020GXNSFGA238001，2018GXNSFAA294090）

Genotype analysis of Cryptococcus gattii species complex in Guangxi, southern China

LI Bingkun¹, HUANG Chunyang¹, LI Xiuying¹, ZHENG Yanqing², PAN Kaisu¹, LIAO Wanqing³, CAO Cunwei¹

1. Department of Dermatology and Venereology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China;
2. Nanning Fourth People's Hospital, Nanning 530023, China;
3. Institute of Dermatology and Mycosis, Shanghai Changzheng Hospital, Shanghai 200003, China

Received:2020-11-23 Published:2021-04-25

摘要/Abstract

摘要： 目的探讨中国广西地区格特隐球菌菌种复合体的基因型特点、种群结构特征和全球菌株的进化关系。方法收集2014—2018年间分离自临床确诊为隐球菌病患者的隐球菌临床株，利用CGB培养基初步筛选格特隐球菌菌种复合体。采用多位点序列分型方法（MLST）确定基因型。通过MEGA7软件构建系统发育树，利用R语言进行主成分分析。使用微量肉汤稀释法M27-A3方案行体外抗真菌药物敏感性检验。结果 120株临床隐球菌中，分离出11株格特隐球菌菌种复合体，6株属于C.deuterogattii（AFLP6/VGⅡ），5株属于C.gattii sensus stricto（AFLP4/VGI）。分离自广西的AFLP6/VGⅡ呈遗传多样性，主要起源进化自南美巴西格特隐球菌菌种复合体。11株分离菌株均对常用抗真菌药物敏感。结论中国广西可能出现高致病性AFLP6/VGⅡ，对格特隐球菌菌种复合体进行有效的全国性监测是必要的。

关键词: 格特隐球菌菌种复合体, 多位点系列分型, 系统发育分析, 抗真菌药物敏感性

Abstract: Objective To explore the genotype characteristics and population structure characteristics of Cryptococcus gattii species complex in Guangxi, China, and to clarify the evolutionary relationship between Guangxi clinical isolates and global strains. Methods Clinical strains of Cryptococci isolated from clinically diagnosed patients with cryptococcosis between 2014 and 2018 were collected, and Cryptococci were preliminarily screened using CGB medium. Multi-site series typing (MLST) was used to determine genotype. Phylogenetic tree construction through MEGA7 software;Principal component analysis using R language. In vitro susceptibility test of antifungal agents was performed using m27-A3 protocol using microbroth dilution. Results Among the 120 strains of clinical Cryptococcus, 11 strains of Cryptococcus gattii species complex were isolated, 6 strains belonged to C.deuterogattii (AFLP6/VGⅡ), and 5 belonged to C.gattii sensus stricto (AFLP4/VGI). Strains of AFLP6/VGⅡ from Guangxi, were genetically variable and mainly originated and evolved from the Brazilian C.deuterogattii in South America. All isolates were sensitive to antifungal drugs. Conclusion Our study suggests that highly pathogenic AFLP6/VGⅡ may occur in Guangxi China, and effective national surveillance of C. gattii species complex is necessary.

Key words: Cryptococcus gattii, MLST, phylogenetic analysis, antifungal susceptibility

中图分类号:

R379.5

李炳坤, 黄春阳, 李秀楹, 郑艳青, 潘开素, 廖万清, 曹存巍. 中国广西地区格特隐球菌菌种复合体的基因型分析[J]. 中国真菌学杂志, 2021, 16(2): 84-89.

LI Bingkun, HUANG Chunyang, LI Xiuying, ZHENG Yanqing, PAN Kaisu, LIAO Wanqing, CAO Cunwei. Genotype analysis of Cryptococcus gattii species complex in Guangxi, southern China[J]. Chinese Journal of Mycology, 2021, 16(2): 84-89.

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