10种中药单体对球形孢子丝菌的体外抗真菌作用研究

中国真菌学杂志　 2020, Vol. 15 　Issue (3): 145-149.

10种中药单体对球形孢子丝菌的体外抗真菌作用研究

曲芯瑶¹, 李珊山², 曲悦³, Abdulhakim Salad Nageye², 宋洋², 崔岩²

1. 长春中医药大学附属医院药物临床实验办公室, 长春 130021;
2. 吉林大学第一医院皮肤性病科, 长春 130021;
3. 梅河口市中心医院中医科, 通化 13500

收稿日期:2019-04-10 出版日期:2020-06-28 发布日期:2020-06-28
通讯作者: 崔岩,E-mail:cui_yan_516@sina.com E-mail:cui_yan_516@sina.com
作者简介:曲芯瑶,女(汉族),硕士,助理研究员.E-mail:quxinyao07@163.com
基金资助:
国家自然科学基金（81703136）和吉林省科技发展计划项目（20170204060SF）

In vitro antifungal susceptibility of 10 Chinese herbal monomers against Sporothrix globosa

QU Xin-yao¹, LI Shan-shan², QU Yue³, Abdulhakim Salad Nageye², SONG Yang², CUI Yan²

1. Good Clinical Practice Office, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun 130021, China;
2. Department of Dermatology and Venereology, First Hospital of Jilin University, Changchun 130021, China;
3. Department of Traditional Chinese Medicine, Meihekou Central Hospital, Tonghua 13500, China

Received:2019-04-10 Online:2020-06-28 Published:2020-06-28

摘要/Abstract

摘要：

目的观察10种中药单体对球形孢子丝菌体外抑菌效果。方法参照CLSI的制M38-A2（3rd），采用微量液基稀释法，检测10种中药单体对20株球形孢子丝菌的作用。结果 10种中药单体最小抑菌浓度（MIC）的几何均数范围为53.82 μg/mL~>1024 μg/mL，大蒜素、小檗碱、苦参碱、蛇床子素和丹皮酚的抑菌活性最佳，几何均数分别依次为53.82 μg/mL、84.45 μg/mL、103.97 μg/mL、137.19 μg/mL和284.05 μg/mL，大蒜素的抗菌作用最强，MIC的范围为16 ~128 μg/mL。最小杀菌浓度（MFC）的范围为64 μg/mL~>1024 μg/mL。大蒜素的MFC值最低，几何均数为415.87 μg/mL。结论大蒜素、小檗碱、苦参碱、蛇床子素和丹皮酚对球形孢子丝菌具有一定的抑菌活性，不同中药单体抑菌活性存在差异。

关键词: 球形孢子丝菌, 中药单体, 药敏试验, 大蒜素

Abstract:

Objective To investigate the antifungal effects of 10 Chinese herbal monomers against Sporothrix globosa in vitro. Methods The effects of 10 Chinese herbal monomers against 20 strains of S. globosa were determined by micro-broth dilution method with reference to CLSI M38-A2 (3rd). Results The geometric mean(GM) of the minimum inhibitory concentration (MIC) of 10 Chinese herbal monomers ranged from 53.82 μg/mL to>1024 μg/mL. The effects of allicin, berberine, matrine, osthole and paeonol were the best, and the geometric mean were 53.82 μg/mL, 84.45 μg/mL, 103.97 μg/mL, 137.19 μg/mL and 284.05 μg/mL, respectively. Allicin was the most active antifungal against S. globosa, showing the range of MIC was 16-128 μg / mL. The minimum fungicidal concentration (MFC) ranged from 64 μg/mL to >1024 μg/mL. Allicin had the lowest MFC value and GM was 415.87 μg/mL. Conclusion Allicin, berberine, matrine, osthole and paeonol could against S. globosa, and the effects among Chinese herbal monomers were different.

Key words: Sporothrix globosa, Chinese herbal monomers, antifungal susceptibility testing, allicin

中图分类号:

曲芯瑶, 李珊山, 曲悦, Abdulhakim Salad Nageye, 宋洋, 崔岩. 10种中药单体对球形孢子丝菌的体外抗真菌作用研究[J]. 中国真菌学杂志, 2020, 15(3): 145-149.

QU Xin-yao, LI Shan-shan, QU Yue, Abdulhakim Salad Nageye, SONG Yang, CUI Yan. In vitro antifungal susceptibility of 10 Chinese herbal monomers against Sporothrix globosa[J]. Chinese Journal of Mycology, 2020, 15(3): 145-149.

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