Calcineurin A基因对马尔尼菲篮状菌致病力的影响

中国真菌学杂志　 2020, Vol. 15 　Issue (3): 138-144.

Calcineurin A基因对马尔尼菲篮状菌致病力的影响

黄春阳^1,3, 潘开素^1,3, 李秀楹¹, 曹存巍^1,3, 郑艳青^2,3

1. 广西医科大学第一附属医院皮肤性病科, 南宁 530021;
2. 南宁市第四人民医院, 南宁 530023;
3. 广西艾滋病防治研究重点实验室, 南宁 530021

收稿日期:2020-02-17 出版日期:2020-06-28 发布日期:2020-06-28
通讯作者: 曹存巍,E-mail:caocunwei@yeah.net;郑艳青,E-mail:dryqzheng@yeah.net E-mail:caocunwei@yeah.net;dryqzheng@yeah.net
作者简介:黄春阳,女(壮族),硕士研究生在读.E-mail:huangchunyangy@163.com;潘开素,女(汉族),硕士研究生.E-mail:pankaisu431@163.com
基金资助:
国家自然科学基金（81960567，81271804），广西自然科学基金（2018GXNSFAA294090，桂科 AB18221017）

The effects of calcineurin A on the pathogenicity of Talaromyces marneffei

HUANG Chun-yang^1,3, PAN Kai-su^1,3, LI Xiu-ying¹, CAO Cun-wei^1,3, ZHENG Yan-qing^2,3

1. Department of Dermatology and Venereology, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China;
2. The Fourth People's Hospital of Nanning, Nanning 530023, China;
3. Guangxi Key Laboratory of AIDS Prevention and Treatment, Nanning 530021, China

Received:2020-02-17 Online:2020-06-28 Published:2020-06-28

摘要/Abstract

摘要：

目的探讨calcineurin A基因（CnaA）对马尔尼菲篮状菌致病力的影响。方法通过观察CnaA基因缺陷菌株（ΔcnaA）形态发生、细胞壁完整性、压力应激、与巨噬细胞共培养后的生长情况，并构建动物感染模型以明确ΔcnaA敲除株致病力特点。结果 ΔcnaA敲除株在形态发生及细胞壁完整性等方面存在缺陷；与巨噬细胞共孵育的ΔcnaA的分生孢子迅速被吞噬，相比于 wild-type，被吞噬24 h后的ΔcnaA菌株生长速度较慢，在48 h时，巨噬细胞内的ΔcnaA菌株大部分被杀灭并分解；ΔcnaA 感染的小鼠死亡率及组织菌载量较wild-type明显降低。结论 CnaA在马尔尼菲篮状菌的致病力中发挥重要的调控作用。

关键词: 马尔尼菲篮状菌, CnaA, 形态发生, 细胞壁完整性, 免疫逃逸, 致病力

Abstract:

Objective To investigate the effects of calcineurin A from T. marneffei on pathogenicity. Methods The morphogenesis, cell wall integrity, external stress responses, growth of co-cultured with macrophages, and constructed animal infection model were observed to determine the roles of the CnaA on the pathogenicity of T. marneffei. Results Deletion of the CnaA gene in T. marneffei resulted in a strain with significant defects in morphogenesis, cell wall integrity, and resistance to various stressors. The conidia of ΔcnaA strain co-incubated with RAW264.7 macrophages were rapidly phagocytosed. By 24 h, the ΔcnaA strain showed much slower growth than the wild-type cells. At 48 h, very few ΔcnaA strain remaining in macrophagesshowed that most of the ΔcnaA strain had been killed and degraded. In the infected mouse model, the mortality and tissue fungi burden of ΔcnaA mutant infected mice were significantly lower than those of wild-type. Conclusion CnaA of T. marneffei plays a key role in controlling fungal morphogenesis and the response to internal and external stresses, as well as in the host immune response and fungal pathogenicity.

Key words: Talaromyces marneffei, CnaA, morphogenesis, cell wall integrity, immune escape, pathogenicity

中图分类号:

R379.9

黄春阳, 潘开素, 李秀楹, 曹存巍, 郑艳青. Calcineurin A基因对马尔尼菲篮状菌致病力的影响[J]. 中国真菌学杂志, 2020, 15(3): 138-144.

HUANG Chun-yang, PAN Kai-su, LI Xiu-ying, CAO Cun-wei, ZHENG Yan-qing. The effects of calcineurin A on the pathogenicity of Talaromyces marneffei[J]. Chinese Journal of Mycology, 2020, 15(3): 138-144.

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