聚六亚甲基双胍体外抗真菌作用研究

中国真菌学杂志　 2020, Vol. 15 　Issue (2): 93-96.

聚六亚甲基双胍体外抗真菌作用研究

谭静文¹, 徐红², 宋金凤¹, 高志琴¹, 杨虹¹, 杨连娟¹, 温海²

1. 上海市皮肤病医院真菌病科, 上海 200443;
2. 海军军医大学附属长征医院皮肤科, 上海 200003

收稿日期:2020-01-07 出版日期:2020-04-28 发布日期:2020-04-28
通讯作者: 杨连娟,E-mail:zhy52808290@qq.com;温海,E-mail:wenhai98@sohu.com E-mail:zhy52808290@qq.com;wenhai98@sohu.com
作者简介:谭静文,女(汉族),硕士研究生,主治医师.E-mail:cecilia_88903@163.com
基金资助:
上海市卫生健康委员会面上项目（201940476）

In vitro antifungal susceptibility of polyhexamethylene biguanide against common superficial fungal species

TAN Jing-wen¹, XU Hong², SONG Jin-feng¹, GAO Zhi-qin¹, YANG Hong¹, YANG Lian-juan¹, WEN Hai²

1. Department of Medical Mycology, Shanghai Dermatology Hospital, 200443 Shanghai, China;
2. Department of Dermatology, Shanghai Changzheng Hospital, Second Affiliated Hospital of Naval Military Medical University, 200003 Shanghai, China

Received:2020-01-07 Online:2020-04-28 Published:2020-04-28

摘要/Abstract

摘要：

目的探讨聚六亚甲基双胍（PHMB）对浅部真菌病常见致病菌的杀灭作用。方法参照标准的微量液基稀释法和琼脂稀释法检测PHMB粉剂和溶液对红色毛癣菌、须癣毛癣菌、犬小孢子菌及白念珠菌临床分离株的体外最低抑菌浓度（MIC）和最低杀菌浓度（MFC）。结果 PHMB对红色毛癣菌、须癣毛癣菌、犬小孢子菌和白念珠菌临床分离株的MIC范围分别为1.0~4.0 μg/mL、2.0~4.0 μg/mL、2.0~4.0 μg/mL和0.3~5.0 μg/mL；MFC范围分别为2.0~8.0 μg/mL、 4.0 μg/mL、2.0~4.0 μg/mL和5.0~10.0 μg/mL。琼脂稀释法结果表明PHMB溶液浓度为1.25%时，红色毛癣菌、犬小孢子菌和须癣毛癣菌无菌落生长；PHMB溶液浓度为0.32%时，白念珠菌无菌落生长。加入透皮剂氮酮后，杀菌效果并无改变。结论 PHMB对浅部真菌病常见的致病真菌皮肤癣菌及白念珠菌具有较好的抑制和杀灭作用。

关键词: 聚六亚甲基双胍, 红色毛癣菌, 须癣毛癣菌, 犬小孢子菌, 白念珠菌

Abstract:

Objective To determine the in vitro antifungal susceptibility of polyhexamethylene biguanide (PHMB) against common superficial fungal species. Methods We used the standard broth microdilution method and agar dilution method to evaluate the minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) of PHMB against the clinical isolates of Trichophyton rubrum, T. mentagrophytes, Microsporum canis and Candida albicans. Results Broth microdilution method showed that MIC range of PHMB powder against T. rubrum was 1.0~4.0 μg/mL, the MFC range was 2.0~8.0 μg/mL. The MIC range of T. mentagrophytes was 2.0~4.0 μg/mL and the MFC was 4.0 μg/mL. To M. canis the MIC range was 2.0~4.0 μg/mL and MFC range was 2.0~4.0 μg/mL. To C. albicans the MIC range turned out to be 0.3~5.0 μg/mL and the MFC range was 5.0~10.0 μg/mL. The agar dilution method showed there were none T. rubrum, T. mentagrophytes and M.canis colony growth on agar plate when PHMB liquid at the concentration of 1.25%. There was none C. albicans colony growth at the concentration of 0.32%. After added the penetration enhancer of azone, the result showed no difference. Conclusions PHMB showed great in vitro efficacy against T. rubrum, T. mentagrophytes, M. canis and C. albicans, suggesting a potential therapeutic choice for treatment of superficial fungal infection.

Key words: polyhexamethylene biguanide (PHMB), Trichophyton rubrum, Trichophyton mentagrophytes, Microsporum canis, Candida albicans

中图分类号:

R978.5

谭静文, 徐红, 宋金凤, 高志琴, 杨虹, 杨连娟, 温海. 聚六亚甲基双胍体外抗真菌作用研究[J]. 中国真菌学杂志, 2020, 15(2): 93-96.

TAN Jing-wen, XU Hong, SONG Jin-feng, GAO Zhi-qin, YANG Hong, YANG Lian-juan, WEN Hai. In vitro antifungal susceptibility of polyhexamethylene biguanide against common superficial fungal species[J]. Chinese Journal of Mycology, 2020, 15(2): 93-96.

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