球形孢子丝菌刺激小鼠树突状细胞表达前炎症细胞因子的研究

摘要/Abstract

摘要：

目的本研究旨在观察分离于新疆的球形孢子丝菌临床株刺激小鼠树突状细胞（Dendritic cells，DCs）后不同炎症因子分泌表达的特征，初步预测这些炎症因子的功能。方法菌株来源于淋巴管型孢子丝菌病患者。将该菌配置成不同浓度的菌悬液（1×10⁴个/mL~1×10⁷个/mL），刺激小鼠DC （细胞悬浮液浓度1×10⁶细胞/mL），分别收集6 h、24 h、48 h、72 h时细胞培养上清液，采用酶免法检测IL-1β、IL-6、IL-4、TNF-α、IFN-γ的含量表达。结果以最低浓度菌液（1×10⁴个/mL）刺激DC，被刺激后的DC分泌了IL-1β、IL-6和TNF-α，不同时间点分泌量分别为：IL-1β（6 h：21.26±3.03；24 h：24.04±4.25；48 h：24.90±4.31；72 h：27.29±6.09）、IL-6（6 h：44.38±3.73；24 h：101.72±12.28；48 h：133.10±8.67；72 h：180.38±13.84）、TNF-α（6 h：860.36±20.64；24 h：356.03±11.46；48 h：457.43±17.39；72 h：1454.53±19.46），但是IFN-γ和IL-4未见分泌表达。IL-1β和IL-6分泌水平有随时间和剂量依赖而逐渐增高，但是TNF-α释放量呈现不规律表达。结论 DC参与了球形孢子丝菌感染的天然免疫应答，分泌的关键炎症因子是IL-1β、IL-6、和TNF-α，表达量为TNF-α > IL-6 > IL-1β。其中IL-1β和IL-6分泌表达量具有时间和剂量依赖性。

关键词: 孢子丝菌病, 球形孢子丝菌, 树突状细胞, 天然免疫

Abstract:

Objective To observe the characteristics of the secretion and expression of different inflammatory factors in mouse dendritic cells(DCs) stimulated by the Sporothrix globosa clinical strain in Xinjiang, and to predict the function of the inflammatory factor. Methods The experimental clinical strain was derived from infected lymphatic sporotrichosis, which was configured into different concentrations of fungal suspension (1×10 ⁴ cells/mL to 1×10⁷ cells/mL) to stimulate mouse DC (cell suspension concentration 1×10⁶ cells/mL), cell culture supernatants at concentrations of 6 hours, 24 hours, 48 hours, and 72 hours were collected, and IL-1β, IL-6, IL-4, TNF-α,and IFN-γ were detected by enzyme immunoassay. Results The stimulated DCs secreted IL-1β, IL-6 and TNF-α, and the secretions at different time points were:IL-1β (6 hours:21.26±3.03; 24 hours:24.04±4.25; 48 hours):24.90 ±4.31; 72 hours:27.29 ±6.09), IL-6 (6 hours:44.38 ±3.73; 24 hours:101.72 ±12.28; 48 hours:133.10 ±8.67; 72 hours:180.38 ±13.84), TNF-α (6 hours:860.36 ±20.64; 24 hours:356.03 ±11.46; 48 hours:457.43 ±17.39; 72 hours:1454.53 ±19.46), but no secretory expression was observed for IFN-γ and IL-4.The secretion levels of IL-1β and IL-6 increased gradually with time and dose, but the release of TNF-α was irregularly expressed. Conclusion DCs are involved in the innate immune response of Sporothrix globosa infection. The key inflammatory factors secreted are IL-1β, IL-6, and TNF-α, and the expression levels are TNF-α > IL-6 > IL-1β. The secretion levels of IL-1β and IL-6 are gradually increased in a time- and dose-dependent manner.

Key words: sporotrichosis, Sporothrix globosa, dendritic cells, innate immunity

中图分类号:

R379.9

王晓东, 迪丽努尔·塔西买买提, 迪丽达尔·地里夏提, 哈地利亚·哈斯木, 帕丽达·阿布利孜. 球形孢子丝菌刺激小鼠树突状细胞表达前炎症细胞因子的研究[J]. 中国真菌学杂志, 2019, 14(6): 321-325.

WANG Xiao-dong, Dlinuer·Taximaimaiti, Dlidaer·Dlixiati, Hadiliya·Hasimu, Paride Abliz. Expression of proinflammatory cytokines in mouse dendritic cells stimulated by Sporothrix globosa[J]. Chinese Journal of Mycology, 2019, 14(6): 321-325.

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