不同激光不同能量对红色毛癣菌的体外抑制评估及部分机制的探讨

中国真菌学杂志　 2019, Vol. 14 　Issue (5): 292-296.

不同激光不同能量对红色毛癣菌的体外抑制评估及部分机制的探讨

熊瑛¹, 陈婷¹, 柴宝², 曾飞鹏², 孙文文¹, 吴波¹

1. 深圳市妇幼保健院皮肤科, 深圳 518000;
2. 深圳市南山区人民医院皮肤科, 深圳 518000

收稿日期:2018-11-19 出版日期:2019-10-28 发布日期:2019-10-28
通讯作者: 吴波,E-mail:13828700253@163.com E-mail:13828700253@163.com
作者简介:熊瑛,女(汉族),硕士,副主任医师.E-mail:251122645@qq.com

In vitro evaluation of inhibitory effect and mechanism of various lasers on Trichophyton rubrum under different energies

XIONG Ying¹, CHEN Ting¹, CHAI Bao², ZENG Fei-peng², SUN Wen-wen¹, WU Bo¹

1. Shenzhen Maternity and Child Healthcare Hospital, Dermatology department, shenzhen 518000;
2. Shenzhen Nan Shan Hospital, Dermatology department, shenzhen 518000

Received:2018-11-19 Online:2019-10-28 Published:2019-10-28

摘要/Abstract

摘要：

目的探讨多种激光在不同的能量下对红色毛癣菌的体外抑制作用以及可能的作用机制。方法将Q开关Nd：YAG 532nm激光、Q开关Nd：YAG 1064nm激光、长脉宽Nd：YAG1064nm激光和强脉冲光分为不同能量组及未照光组，体外照射红色毛癣菌的菌落，观察第0、1、3、7天时菌落直径的变化差异，用酶联免疫吸附测定激光照射后红色毛癣菌产生角蛋白酶的活性。结果与未照光组相比，上述激光在低能量时对红色毛癣菌的菌落直径与角蛋白酶活性无明显影响。2~4J/cm²能量Q开关Nd：YAG 532nm激光、4~8J/cm²能量Q开关Nd：YAG 1064nm激光、4J/cm²长脉宽Nd：YAG 1064nm激光能显著抑制菌落的生长，红色毛癣菌角蛋白酶的活性出现明显下降。结论 2~4J/cm²能量Q开关Nd：YAG 532nm激光、4~8J/cm²能量Q开关Nd：YAG 1064nm激光、4J/cm²长脉宽Nd：YAG 1064nm激光能抑制红色毛癣菌生长及角蛋白酶的活性。

关键词: 激光, 甲真菌病, 红色毛癣菌

Abstract:

Objective To investigate inhibitory effect of various lasers on Trichophyton rubrum under different energies, and explore the possible mechanism. Methods Plates of standard size of T.rubrum colonies were assigned as control or experimental Groups. Experimental group was irradiated by different lasers, which including Q-switched Nd:YAG 532nm laser, Q-switched Nd:YAG 1064nm laser, long pulse width Nd:YAG 1064nm laser and intense pulsed light. The effects of different lasers energies on isolated colonies were assessed. After irradiation, the sizes of colonies were measured at 1^st, 3^rd and 7^th days, the degree of keratinase production was measured by enzyme-linked immunosorbent assay. Results Compared with the control group, low power lasers did not have any inhibitory effect on the growth and keratinase production of Trichophyton rubrum. Q-switched Nd:YAG 532nm laser at 2-4J/cm²,Q-switched Nd:YAG 1064nm laser at 4-8J/cm² and long pulse width Nd:YAG 1064nm laser at 4J/cm² could significantly inhibit the growth of colonies and the production of keratinase. Conclusion Growth of T.rubrum and the production of keratinase could be effectively suppressed by Q-switched Nd:YAG 532nm laser at 2-4J/cm², Q-switched Nd:YAG 1064nm laser at 4-8J/cm² and long pulse width Nd:YAG 1064nm laser at 4J/cm².

Key words: laser, onychomycosis, Trichophyton rubrum

中图分类号:

R379.2

熊瑛, 陈婷, 柴宝, 曾飞鹏, 孙文文, 吴波. 不同激光不同能量对红色毛癣菌的体外抑制评估及部分机制的探讨[J]. 中国真菌学杂志, 2019, 14(5): 292-296.

XIONG Ying, CHEN Ting, CHAI Bao, ZENG Fei-peng, SUN Wen-wen, WU Bo. In vitro evaluation of inhibitory effect and mechanism of various lasers on Trichophyton rubrum under different energies[J]. Chinese Journal of Mycology, 2019, 14(5): 292-296.

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