国产卡泊芬净对曲霉菌的体外活性评估

中国真菌学杂志　 2019, Vol. 14 　Issue (3): 141-146.

国产卡泊芬净对曲霉菌的体外活性评估

于淑颖^1,2, 张丽¹, 李颖^1,2, 徐英春¹

1. 中国医学科学院北京协和医院检验科侵袭性真菌病机制研究与精准诊断北京市重点实验室, 北京 100730;
2. 中国医学科学院北京协和医学院, 北京 100730

收稿日期:2018-11-05 出版日期:2019-06-28 发布日期:2019-06-28
通讯作者: 徐英春,E-mail:xycpumch@139.com E-mail:xycpumch@139.com
作者简介:于淑颖(1993-),女(民族).博士研究生,E-mail:yushuying2016@163.com
基金资助:
国家自然科学基金（81572057），北京协和医学院创新工程基金（2018-1002-01-02）

Evaluation of the in vitro antifungal activity of the domestic caspofungin against clinical Aspergillus isolates

YU Shu-ying^1,2, ZHANG Li¹, LI Ying^1,2, XU Ying-chun¹

1. Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Department of Clinical Laboratory, Peking Union Medical College Hospital, Beijing;
2. Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China

Received:2018-11-05 Online:2019-06-28 Published:2019-06-28

摘要/Abstract

摘要：

目的以进口卡泊芬净（原研药）为对照，评估国产卡泊芬净（仿制药）对曲霉菌的体外抗菌活性及与进口卡泊芬净的一致性。方法利用北京协和医院分离的75株侵袭性曲霉菌，采用CLSI推荐的微量液基稀释法对8种抗真菌药物进行体外敏感性试验。结果棘白菌素类药物的MEC值总体低于唑类药物的MIC值，而米卡芬净的MEC₅₀和MEC₉₀比卡泊芬净低出2个梯度。除了对1株黄曲霉的MIC值为4 μg/mL，两性霉素B对其余74株菌株的MIC值≤ 2 μg/mL。全部菌株对伏立康唑和泊沙康唑均敏感，分别有16株烟曲霉、2株黄曲霉和5株黑曲霉对伊曲康唑耐药。国产卡泊芬净和进口卡泊芬净仅对11株曲霉菌的MEC值存在不一致，且国产卡泊芬净仅较卡进口泊芬净只低一个浓度梯度。卡泊芬净和米卡芬净对超过76%的曲霉菌24 h和48 h的药敏测定结果完全一致。结论相较于唑类药物和两性霉素B，卡泊芬净对曲霉菌具有很好的体外抗真菌活性，且国产卡泊芬净与进口卡泊芬净的体外抗菌活性基本高度一致。

关键词: 卡泊芬净, 药物敏感性, 曲霉菌, 一致性

Abstract:

Objective To evaluate the in vitro antifungal activity and consistency of original and domestic caspofungin against clinical Aspergillus isolates. Methods A total of 75 clinical Aspergillus isolates were collected from Peking Union Medical College Hospital. The antifungal susceptibility testing was carried out using the microdilution method recommended by CLSI. Results The MEC values of echinocandins were generally lower than the MIC values of the azoles, while MEC₅₀ and MEC₉₀ of micafungin were lower two dilutions than that of caspofungin. Except for one Aspergillus flavus isolates with MIC of 4 μg/mL, the MICs of remaining 74 Aspergillus isolates to amphotericin B were no more than 2 μg/mL. All the Aspergillus isolates were susceptible to voriconazole and posaconazole, but 16 Aspergillus fumigatus, 2 Aspergillus flavus and 5 Aspergillus niger isolates were resistant to itraconazole. The MEC values of original and domestic caspofungin against 11 Aspergillus isolates were inconsistent, but the MEC values of domestic caspofungin were only lower one dilution than those of original caspofungin. The consistency occurred in the antifungal susceptibility of over 76% of Aspergillus isolates to caspofungin and micafungin between 24h and 48h results. Conclusions Compared with azoles and amphotericin B, caspofungin had good in vitro antifungal activity against Aspergillus isolates. and domestic caspofungin was highly consistent with original caspofungin.

Key words: caspofungin, antifungal susceptibility, Aspergillus, consistency

中图分类号:

于淑颖, 张丽, 李颖, 徐英春. 国产卡泊芬净对曲霉菌的体外活性评估[J]. 中国真菌学杂志, 2019, 14(3): 141-146.

YU Shu-ying, ZHANG Li, LI Ying, XU Ying-chun. Evaluation of the in vitro antifungal activity of the domestic caspofungin against clinical Aspergillus isolates[J]. Chinese Journal of Mycology, 2019, 14(3): 141-146.

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