关键词: 隐球菌感染, DNA芯片, 分子诊断

Abstract:

Objective To diagnose the case of meningitis suspected of cryptococcal infection by molecular detection technique. Methods Cerebrospinal fluid samples of patients were collected, DNA was extracted, primers were designed for PCR amplification, and gene detection was performed on the amplified products by DNA chip technology. Results Sample showed positive results for Cryptococcus neoformans. Conclusion PCR amplification of primers designed from ITS conserved sequence and non-culture detection of cerebrospinal fluid samples by DNA chip technology have clinical reference value for deep fungal infections that are difficult to diagnose by traditional methods.

Key words: Cryptococcus infection, DNA chip, molecular diagnosis