基于定量蛋白质组学的NT-89抗白念珠菌作用机制研究

中国真菌学杂志　 2019, Vol. 14 　Issue (2): 70-77.

基于定量蛋白质组学的NT-89抗白念珠菌作用机制研究

刘昱, 阎澜, 姜远英

海军军医大学药学系军特药研究中心, 上海 200433

收稿日期:2018-03-14 出版日期:2019-04-28 发布日期:2019-04-28
通讯作者: 阎澜,E-mail:ylan20001228@sina.com;姜远英,E-mail:13761571578@163.com E-mail:ylan20001228@sina.com;13761571578@163.com
作者简介:刘昱,男(汉族),硕士研究生在读.E-mail:liuyu120906@163.com
基金资助:
国家自然科学基金（81573473），上海市自然科学基金（17ZR1437700）

Study on the mechanism of NT-89 against Candida albicans based on quantitative proteomics

LIU Yu, YAN Lan, JIANG Yuan-ying

Military Special Medicine Research Center, Department of Pharmacy, Naval Medical University, Shanghai 200433, China

Received:2018-03-14 Online:2019-04-28 Published:2019-04-28

摘要/Abstract

摘要：

目的利用定量蛋白质组学iTRAQ技术，分析抗真菌化合物NT-89作用后白念珠菌蛋白质组的含量变化。方法提取NT-89作用前后的白念珠菌总蛋白与细胞壁蛋白，利用iTRAQ技术检测蛋白提取物中蛋白质的相对丰度，寻找药物作用前后的差异蛋白，并利用GO数据库注释蛋白质功能分类。结果总蛋白（TP）提取物中检测出295种差异蛋白，其中的Ywp1p、Pga10p在总蛋白中含量下调最为显著。细胞壁蛋白（CWP）提取物中有6种GPI锚定蛋白含量显著降低。结论 NT-89影响了白念珠菌细胞壁的结构完整与功能，iTRAQ技术能够为药物的作用机制研究提供有效参考信息。

关键词: NT-89, 白念珠菌, 细胞壁, 氟化氢吡啶, GPI锚定蛋白, iTRAQ

Abstract:

Objective To analyze the differentially expressed proteins after the treatment of anti-fungal compound NT-89 against Candida albicansby quantitative proteomics iTRAQ technology. Methods Total protein and cell wall proteins of C. albicans were extracted with or without NT-89 treatment. The relative abundanceand differential proteins in protein extracts were detected by iTRAQ. Then the function classification of the differential proteins was performed using GO database. Results 295 differential proteins were detected in extract of total protein (TP), and Ywp1p and Pga10p were most significantly down-regulated in total protein. 6 GPI-anchored proteins in cell wall protein (CWP) extract were found significantly down-regulated. Conclusion NT-89 disrupted the structural integrity and function of cell wall in C. albicans. The iTRAQ technology can provide effective reference information for drug mechanism research.

Key words: NT-89, Candida albicans, cell wall, HF-pyridine, GPI-anchored protein, iTRAQ

中图分类号:

刘昱, 阎澜, 姜远英. 基于定量蛋白质组学的NT-89抗白念珠菌作用机制研究[J]. 中国真菌学杂志, 2019, 14(2): 70-77.

LIU Yu, YAN Lan, JIANG Yuan-ying. Study on the mechanism of NT-89 against Candida albicans based on quantitative proteomics[J]. Chinese Journal of Mycology, 2019, 14(2): 70-77.

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