日舒安洗液联合克霉唑治疗小鼠外阴阴道念珠菌病的疗效观察

中国真菌学杂志　 2019, Vol. 14 　Issue (1): 32-36.

日舒安洗液联合克霉唑治疗小鼠外阴阴道念珠菌病的疗效观察

刘道凡¹, 伍启康²

1. 广东省佛山市第一人民医院皮肤科, 佛山 528000;
2. 广东省佛山市第一人民医院检验科, 佛山 528000

收稿日期:2018-05-24 出版日期:2019-02-28 发布日期:2019-02-28
作者简介:刘道凡,男(汉族),硕士,副主任医师.E-mail:peihongmintl000@163.com
基金资助:
广东省医学科学技术研究基金项目（A2016544）

The efficacy of Rishuan lotion combined with clotrimazole on treatment of vulvovaginal candidiasis in mice

LIU Dao-fan¹, WU Qi-kang²

1. Department of Dermatology, The first people's Hospital of Foshan, Foshan 528000, China;
2. Department of Laboratory, The first people's Hospital of Foshan, Foshan 528000, China

Received:2018-05-24 Online:2019-02-28 Published:2019-02-28

摘要/Abstract

摘要：

目的观察日舒安洗液联合克霉唑治疗小鼠外阴阴道念珠菌病的疗效。方法将95只BALB/c雌鼠（8周龄）随机分为A组（5只，空白对照组），B组（30只，模型对照组），C组（30只，克霉唑组）和D组（30只，日舒安洗液联合克霉唑组）。对B组、C组和D组小鼠阴道接种白念珠菌。对A组和B组予等量的生理盐水冲洗阴道，C组单用克霉唑治疗，对D组进行日舒安洗液联合克霉唑治疗。在接种前及治疗过程中观察各组小鼠体重变化。B组、C组和D组在治疗后的第1、2、3、6、8、10、14和16天取阴道灌洗液进行菌落培养，采用涂布法检测小鼠的阴道真菌载荷量，并通过ELISA检测IL-2和IL-8的水平；qRT-PCR法检测小鼠阴道组织中NLRP3、ASC、caspase-1的mRNA表达。结果在前8 d，4组小鼠的体重均保持稳定，接种后B、C和D组小鼠体重下降，随后体重逐渐上升，接近接种前水平。与空白对照组A相比较，B、C和D组阴道灌洗液中阴道真菌载荷量增加，但B、C和D组之间存在统计学差异，D组阴道真菌载荷量少于B组和C组，C组阴道真菌载荷量少于B组；与空白对照组相比较，B、C和D组阴道灌洗液中IL-2和IL-8增加，但治疗后B、C和D组之间IL-2和IL-8水平存在统计学差异，D组IL-2和IL-8水平显著小于C组， C组IL-2和IL-8水平显著小于B组（P<0.01）。与空白对照组相比较，B、C和D组阴道组织中NLRP3、ASC、caspase-1 mRNA表达上调，但B、C和D组之间存在统计学差异，B组mRNA上调水平大于C组和D组，C组mRNA上调水平大于D组（P<0.01）。结论日舒安洗液联合克霉唑对小鼠外阴阴道念珠菌病治疗作用显著。

关键词: 克霉唑, 阴道念珠菌病, 细胞因子

Abstract:

Objective To explore the curative effect of Rishuan lotion (the main component includes kushen) combined with clotrimazole on the treatment of murine vulvovaginal candidiasis. Methods A total of 95 BALB/c female rats (8 weeks old) were randomly divided into four groups, including group A (n=5, blank control group), group B (n=30, model control group), group C (n=30, clotrimazole group) and group D (n=30, Rishuan lotion plus clotrimazole group). Mice in group B,C and D were inoculated with Candida albicans vagina. Mice in group A and B were given equal amount of normal saline to wash the vagina, while mice in group C and D were treated with clotrimazole, Rishuan lotion combined with clotrimazole, respectively. The weight changes of mice in each group were observed before and during the treatment. In group B,C and D, the vaginal lavage fluid was cultured at 1, 2, 3, 6, 8, 10, 14 and 16 days. The vaginal fungal load of mice was detected by coating method, and the levels of IL-2 and IL-8 were detected by ELISA. The expression of NLRP3, ASC and caspase-1 in the vaginal tissue of mice was detected by qRT-PCR. Results At the first 8 days, the weight of mice in the four groups remained stable. After inoculation, the weight of mice in group B,C and D decreased, and then gradually increased, which was close to the level before inoculation. Compared with group A, vaginal fungal load increased in group B, C and D. But there was a statistical difference in vaginal fungal load between group B, C and D. Vaginal fungal load in group D was less than that in group B and group C, while the load in in group C was less than that in group B. Compared with blank control group, IL-2 and IL-8 increased in vaginal lavage fluid of group B, C and D. However, there was a significant difference in the levels of IL-2 and IL-8 in group B, C and D after treatment. The levels of IL-2 and IL-8 in group D were significantly lower than those in group C, and the levels of IL-2 and IL-8 in group C were significantly lower than those in group B (P<0.01). Compared with the blank control group, the expression of NLRP3, ASC and caspase-1mRNA in vagina tissue of group B, C and D was up-regulated. But there was a statistical difference among the three groups. The mRNA expression level in group B was higher than that in group C and group D, and the expression level in group C was higher than that in group D (P<0.01). Conclusion The effect of Rishuan lotion combined with clotrimazole on the treatment of murine vulvovaginal candidiasis was significant.

Key words: Rishuan lotion, clotrimazole, vaginal candidiasis, cytokine

中图分类号:

R519.3

刘道凡, 伍启康. 日舒安洗液联合克霉唑治疗小鼠外阴阴道念珠菌病的疗效观察[J]. 中国真菌学杂志, 2019, 14(1): 32-36.

LIU Dao-fan, WU Qi-kang. The efficacy of Rishuan lotion combined with clotrimazole on treatment of vulvovaginal candidiasis in mice[J]. Chinese Journal of Mycology, 2019, 14(1): 32-36.

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