肺曲霉球1例及实验研究

中国真菌学杂志　 2019, Vol. 14 　Issue (1): 24-27.

肺曲霉球1例及实验研究

邵锦, 余进, 万喆, 李若瑜, 王爱平

北京大学第一医院皮肤性病科北京大学真菌和真菌病研究中心皮肤病分子诊断北京市重点实验室, 北京 100034

收稿日期:2018-04-23 出版日期:2019-02-28 发布日期:2019-02-28
通讯作者: 王爱平,E-mail:wangap516@163.com E-mail:wangap516@163.com
作者简介:邵锦,女(汉族),硕士研究生在读.E-mail:1334172109@qq.com
基金资助:
北京市自然科学基金（7164255）

Pulmonary aspergilloma: a case report and laboratory study

SHAO Jin, YU Jin, WAN Zhe, LI Ruo-yu, WANG Ai-ping

Department of Dermatology, Peking University First Hospital;Research Center for Medical Mycology, Peking University;Beijing Key Laboratory of Molecular Diagnosis on Dermatoses, Beijing 100034, China

Received:2018-04-23 Online:2019-02-28 Published:2019-02-28

摘要/Abstract

摘要：

目的对1例未进行真菌培养的肺真菌球患者术后的组织病理进行实验研究以确定病原菌属。方法 1例咯血并左肺上叶占位的患者接受了左肺上叶切除术，术后组织病理诊断肺真菌球，曲霉感染可能性大，但病变组织未进行真菌培养。本研究对其组织病理标本分别进行了真菌荧光染色、免疫组化染色和组织PCR。结果真菌荧光染色清楚地显示亮蓝色有隔菌丝，呈锐角分支；免疫组织化学染色提示病原菌为曲霉属；组织PCR检测结果阴性。结论当病变组织未进行真菌培养时，免疫组化技术有助于确定病原菌属。

关键词: 肺真菌球, 曲霉, 真菌荧光染色法, 免疫组化, 组织PCR

Abstract:

Objective Laboratory study was performed on the surgical specimens of a pulmonary fungus ball patient without fungal culture to determine the pathogen. Methods A patient with hemoptysis was found lung space occupying lesion on left upper lung and received left upper lobectomy. The postoperative histopathologic diagnosis was pulmonary fungus ball (The pathogenic fungi was the most likely Aspergillus) and the tissue was not used for fungal culture. In this study, fungal fluorescence staining, immunohistochemistry and tissue PCR assays were carried on histopathological specimens. Results Fungal fluorescence staining showed bright blue septate mycelium, with sharp-angle branch. The results of immunohistochemistry suggested that the pathogen was Aspergillus. Tissue PCR result was negative. Conclusion When lacking of fungal culture results, immunohistochemistry techniques might help determine the genus of pathogenic fungi, which was helpful for clinical diagnosis and treatment.

Key words: pulmonary fungus ball, Aspergillus, fungal fluorescence staining, immunohistochemistry, tissue PCR

中图分类号:

R519.8

邵锦, 余进, 万喆, 李若瑜, 王爱平. 肺曲霉球1例及实验研究[J]. 中国真菌学杂志, 2019, 14(1): 24-27.

SHAO Jin, YU Jin, WAN Zhe, LI Ruo-yu, WANG Ai-ping. Pulmonary aspergilloma: a case report and laboratory study[J]. Chinese Journal of Mycology, 2019, 14(1): 24-27.

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