白念珠菌SIM1基因敲除与功能研究

中国真菌学杂志　 2018, Vol. 13 　Issue (4): 223-228.

白念珠菌SIM1基因敲除与功能研究

张晓龙, 鲁仁义, 阎澜, 姜远英

中国人民解放军海军军医大学药学院新药研究中心, 上海 200433

收稿日期:2018-01-27 出版日期:2018-08-28 发布日期:2018-08-28
通讯作者: 姜远英,E-mail:13761571578@163.com;阎澜,E-mail:ylan20001228@sina.com E-mail:13761571578@163.com;ylan20001228@sina.com
作者简介:张晓龙,男(汉族),硕士研究生在读.E-mail:zhangxl0255@163.com
基金资助:
国家自然科学基金面上项目（81573473）

Disruption and functional characterization of SIM1 gene in Candida albicans

ZHANG Xiao-long, LU Ren-yi, YAN Lan, JIANG Yuan-ying

Center for New Drug Research, School of Pharmacy, Naval Medical University, Shanghai 200433, China

Received:2018-01-27 Online:2018-08-28 Published:2018-08-28

摘要/Abstract

摘要：

目的构建白念珠菌SIM1基因缺失菌，初步考察SIM1基因的功能。方法采用同源重组的方法构建SIM1基因双臂缺失菌，通过测定生长曲线、菌丝诱导、黏附上皮细胞等实验考察SIM1基因缺失菌表型。结果成功构建SIM1基因缺失菌，SIM1基因缺失后没有显著影响白念珠菌生长繁殖、菌丝及被膜形成，但白念珠菌对Caco-2细胞和KB细胞的黏附能力显著下降，对部分药物的敏感性增加。结论白念珠菌SIM1基因缺失导致细胞壁成分改变，并影响白念珠菌对宿主的黏附。

关键词: 白念珠菌, SIM1基因, 基因敲除, 细胞壁, 黏附

Abstract:

Objective To construct deletion mutants for Candida albicans SIM1 and explore its functions. Methods Using homologous recombination method to construct deletion mutants for Candida albicans SIM1, by measuring the growth curve, hyphae induction, adhesion epithelial cells and conducting other experiments to observe its phenotypes. Results Gene deletion mutants of SIM1 were successfully constructed. Deletion mutants of SIM1 did not significantly affect the growth and reproduction, hyphae and biofilms formation of Candida albicans, but its adhesive ability to Caco-2 cells and KB cells was obviously impaired. Furthermore deletion mutants of SIM1 were more sensitive to some drugs. Conclusions Deletion mutants of SIM1 result in changes in cell wall composition and affect the adhesion of Candida albicans to hosts.

Key words: Candida albicans, SIM1, gene knockout, cell wall, adhesion

中图分类号:

R379.4

张晓龙, 鲁仁义, 阎澜, 姜远英. 白念珠菌SIM1基因敲除与功能研究[J]. 中国真菌学杂志, 2018, 13(4): 223-228.

ZHANG Xiao-long, LU Ren-yi, YAN Lan, JIANG Yuan-ying. Disruption and functional characterization of SIM1 gene in Candida albicans[J]. Chinese Journal of Mycology, 2018, 13(4): 223-228.

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