球形孢子丝菌黑素抵御抗真菌药物杀伤作用的研究

中国真菌学杂志　 2018, Vol. 13 　Issue (4): 193-196,212.

论著下一篇

球形孢子丝菌黑素抵御抗真菌药物杀伤作用的研究

崔岩¹, 李珊山¹, 侯媛媛², 宋洋¹, 陈瑞丽¹, 李洪霞¹

1. 吉林大学第一医院皮肤性病科, 长春 130021;
2. 吉林大学第一医院儿科, 长春 130021

收稿日期:2018-03-15 出版日期:2018-08-28 发布日期:2018-08-28
通讯作者: 李洪霞,E-mail:724586922@qq.com E-mail:724586922@qq.com
作者简介:崔岩,男,汉族,讲师,E-mail:cui_yan_516@sina.com
基金资助:
吉林省卫生科研计划（2013Z073）；国家自然科学基金（81573060，81703136）；吉林省科技发展计划项目（20160101047JC）。

The mechanism of Sporothrix globosa melanins protect against antifungal drugs

CUI Yan¹, LI Shan-shan¹, HOU Yuan-yuan², SONG Yang¹, CHEN Rui-li¹, LI Hong-xia¹

1. Department of Dermatology and Venereology, First Hospital of Jilin University, Changchun 130021, China;
2. Department of Pediatrics, First Hospital of Jilin University, Changchun 130021, China

Received:2018-03-15 Online:2018-08-28 Published:2018-08-28

摘要/Abstract

摘要：

目的探讨球形孢子丝菌黑素对抗真菌药特比萘芬（TRB）、氟康唑（FCZ）、伊曲康唑（ICZ）和两性霉素B（AMB）的作用。方法利用琼脂平板涂布法比较球形孢子丝菌黑素野生株（MEL⁺）与白化突变株（MEL^-）对抗真菌药物的敏感性；利用紫外分光光度法，检测黑素对抗真菌药浓度的影响；利用HPLC法，分析黑素对抗真菌药的作用机制。结果药敏结果显示，在AMB的（4~16）μg/mL和ICZ的（0.06~0.25）μg/mL浓度间，MEL^-生存率显著低于MEL⁺；紫外分光光度法检测显示，黑素可以显著降低AMB的浓度（P<0.01）；HPLC图谱显示，黑素加入后AMB出现新峰。结论球形孢子丝菌黑素能够抵御AMB和ICZ对菌株的杀伤，并提示黑素可能对AMB进行转化，从而降低药物浓度。

关键词: 球形孢子丝菌, 黑素, 抗真菌药, 药物敏感性, 两性霉素B

Abstract:

Objective To investigate the effects of Sporothrix globosa melanins on four antifungal agents (TRB, FCZ, ICZ and AMB). Methods The agar plate coating method was used to compare the susceptibility of wild-type strain (MEL⁺) and albino mutant (MEL^-) to antifungal drugs; the effect of melanin on the antifungal drug was detected by ultraviolet spectrophotometry. The HPLC was used to analyze the mechanism of melanins against fungal drugs. Results Antifungal drugs susceptibility results showed that the MEL^- survival rate was significantly lower than MEL⁺ at concentrations of 4~16 μg/mL for AMB and 0.06-0.25 μg/mL for ICZ; the results of UV spectrophotometry assay showed that melanin could significantly reduce the concentration of AMB (P<0.01); the chromatogram of HPLC showed that, compared with control group, new peak was appeared after the addition of melanin.Conclusion Sporothrix globosa melanins can protect against AMB and ICZ, and reduce AMB concentration through transforming, probably.

Key words: Sporothrix globosa, melanin, antifungal drug, susceptibility testing, amphotericin B

中图分类号:

R379.9

崔岩, 李珊山, 侯媛媛, 宋洋, 陈瑞丽, 李洪霞. 球形孢子丝菌黑素抵御抗真菌药物杀伤作用的研究[J]. 中国真菌学杂志, 2018, 13(4): 193-196,212.

CUI Yan, LI Shan-shan, HOU Yuan-yuan, SONG Yang, CHEN Rui-li, LI Hong-xia. The mechanism of Sporothrix globosa melanins protect against antifungal drugs[J]. Chinese Journal of Mycology, 2018, 13(4): 193-196,212.

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球形孢子丝菌黑素抵御抗真菌药物杀伤作用的研究

The mechanism of Sporothrix globosa melanins protect against antifungal drugs

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