HIV感染和非HIV感染人群抗耶氏肺孢子菌主要表面糖蛋白的IgG和IgM抗体水平调查

中国真菌学杂志　 2017, Vol. 12 　Issue (6): 342-345.

HIV感染和非HIV感染人群抗耶氏肺孢子菌主要表面糖蛋白的IgG和IgM抗体水平调查

郭爽¹, 刘意², 田小军¹, 栗绍刚¹, 宋营改³, 任翊¹

1. 首都医科大学附属北京友谊医院北京热带医学研究所热带病防治研究北京市重点实验室, 北京 100050;
2. 首都医科大学附属北京佑安医院检验科, 北京 100069;
3. 北京大学第一医院皮肤性病科, 北京 100034

收稿日期:2017-03-22 出版日期:2017-12-28 发布日期:2017-12-28
通讯作者: 任翊,E-mail:ren7016@msn.com;宋营改,E-mail:syg3515@163.com E-mail:ren7016@msn.com;syg3515@163.com
作者简介:郭爽,女(汉族),硕士研究生在读.E-mail:gshdaydayup@163.com
基金资助:
首都医科大学基础临床科研合作基金（17JL03）

Quantitative levels of anti-Pneumocystis jirovecii major surface glycoprotein IgG and IgM antibodies in HIV and non-HIV groups

GUO Shuang¹, LIU Yi², TIAN Xiao-jun¹, LI Shao-gang¹, SONG Ying-gai³, REN Yi¹

Capital Medical University, Beijing Friendship Hospital, Beijing Tropical Medicine Research Institute, Beijing Key Laboratory for Research on Prevention and Treatment of Tropical Disease, Beijing 100050, China

Received:2017-03-22 Online:2017-12-28 Published:2017-12-28

摘要/Abstract

摘要：

目的对北京地区HIV和非HIV人群抗耶氏肺孢子菌（Pneumocystis jirovecii，Pj）主要表面糖蛋白（Major surface glycoprotein，Msg）IgG、IgM抗体滴度水平进行调查，探索其在流行病学调查中的价值。方法利用重组Msg融合蛋白作为抗原建立酶联免疫吸附试验（Enzyme-linked immunosorbent assay，ELISA），用阴性标准血清等比例稀释样本血清建立相对定量抗体检测方法，对176例HIV感染者和226例非HIV人群血清样本进行抗Pj Msg IgG和IgM抗体定量水平调查，所有入选患者临床均不诊断肺孢子菌肺炎（Pneumocystis pneumonia，PCP）。结果抗Pj Msg IgG抗体在非HIV人群中阳性率为42.5%（113/266），113例阳性标本中1：100、1：200、1：400和1：800滴度分别为77例（68.1%）、15例（13.3%）、6例（5.3%）和15例（13.3%）；抗Pj Msg IgG抗体在HIV感染者中阳性率为24.4%（43/176），43例阳性标本中1：100、1：200、1：400和1：800滴度分别为28例（65.1%）、10例（23.3%）、3例（7.0%）和2例（4.7%），与非HIV组相比差异无统计学意义（χ²=4.254，P=0.235）。抗Pj Msg IgM抗体在非HIV人群中阳性率为41.7%（111/266），111例阳性标本中1：100、1：200、1：400和1：800滴度分别为57例（51.4%）、21例（18.9%）、19例（7.1%）和14例（12.6%）；抗Pj Msg IgM抗体在HIV感染者中阳性率为12.5%（22/176），22例阳性标本中1：100、1：200、1：400和1：800滴度分别为10例（45.5%）、2例（9.1%）、4例（18.2%）和6例（27.3%），与非HIV组相比差异无统计学意义（χ²=3.788，P=0.285）。结论非PCP人群中抗Pj Msg IgG抗体定量水平主要集中在1：100和1：200低滴度水平区间，抗Pj Msg抗体水平的定量检测方法及其临床意义值得进一步研究。

关键词: 耶氏肺孢子菌, 主要表面糖蛋白, 抗体, 定量

Abstract:

Objective To investigate the quantitative levels of anti-Pneumocystis jirovecii (Pj) major surface glycoprotein (Msg) IgG and IgM antibodies in HIV and non-HIV groups to explore its value in epidemiological investigation.Method One target sequence of Pj Msg gene was selected and expressed using prokaryotic expression system.Enzyme-linked immunosorbent assay (ELISA) was established to detect anti-Pj Msg IgG and IgM antibodies using recombinant Pj Msg as antigen.Multiple proportion dilution methods were established to measure quantitative levels of anti-Pj Msg IgG and IgM antibodies.A cohort of individuals with HIV infection (n=176) and non HIV controls (n=266) in Beijing China were investigated.All patients were not diagnosed with a situation of pneumocystis pneumonia (PCP).Results The positive rate of anti-Pj Msg IgG antibody in non-HIV group was 42.5% (113/266).Among these 113 positive cases,there were 77 cases (68.1%),15 cases (13.3%),6 cases (5.3%),and 15 cases (13.3%) in the titer of 1:100,1:200,1:400,and 1:800 levels respectively.The positive rate of anti-Pj Msg IgG antibody in HIV infection group was 24.4% (43/176).Among these 43 positive cases,there were 28 cases (65.1%),10 cases (23.3%),3 cases (7.0%),and 2 cases (4.7%) in the titer of 1:100,1:200,1:400,and 1:800 levels respectively.There was no statistical significance in the difference of anti-Pj Msg IgG antibody titer levels compared with non-HIV controls (χ²=4.254,P=0.235).The positive rate of anti-Pj Msg IgM antibody in non-HIV group was 41.7% (111/266).Among these 111 positive cases,there were 57 cases (51.4%),21 cases (18.9%),19 cases (7.1%),and 14 cases (12.6%) in the titer of 1:100,1:200,1:400,and 1:800 levels respectively.The positive rate of anti-Pj Msg IgM antibody in HIV infection group was 12.5% (22/176).Among these 22 positive cases,there were 10 cases (45.4%),2 cases (9.1%),4 cases (18.2%),and 6 cases (27.3%) in the titer of 1:100,1:200,1:400,and 1:800 levels respectively.There was no statistical significance in the difference of anti-Pj Msg IgM antibody titer levels compared with non-HIV controls (χ²=3.788,P=0.285).Conclusions Among these non-PCP individuals,the anti-Pj Msg IgG antibody titer levels were mostly between 1:100 and 1:200.The methods of antibody quantitative levels and their clinic significance need further studies.

Key words: Pneumocystis jirovecii, major surface glycoprotein, antibody, quantitative level

中图分类号:

R519

郭爽, 刘意, 田小军, 栗绍刚, 宋营改, 任翊. HIV感染和非HIV感染人群抗耶氏肺孢子菌主要表面糖蛋白的IgG和IgM抗体水平调查[J]. 中国真菌学杂志, 2017, 12(6): 342-345.

GUO Shuang, LIU Yi, TIAN Xiao-jun, LI Shao-gang, SONG Ying-gai, REN Yi. Quantitative levels of anti-Pneumocystis jirovecii major surface glycoprotein IgG and IgM antibodies in HIV and non-HIV groups[J]. Chinese Journal of Mycology, 2017, 12(6): 342-345.

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