3种微孢子虫在我国西南部分地区腹泻患者中的流行情况调查

中国真菌学杂志　 2017, Vol. 12 　Issue (5): 257-261.

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3种微孢子虫在我国西南部分地区腹泻患者中的流行情况调查

邱璐瑶^1,2, 平静^1,2, 李文道^1,2, 丁嵩涛¹, 刘含登¹

1. 重庆医科大学基础医学实验中心, 重庆 400331;
2. 重庆医科大学儿科学院, 重庆 400331

收稿日期:2017-05-24 出版日期:2017-10-28 发布日期:2017-10-28
通讯作者: 刘含登,E-mail:hdliu@cqmu.edu.cn E-mail:hdliu@cqmu.edu.cn
作者简介:邱璐瑶,女(汉族),本科在读.E-mail:AprilQiu@stu.cqmu.edu.cn
基金资助:
重庆市教委科学技术研究项目（KJ1600202）；重庆医科大学大学生科研与创新实验项目（201619）

Investigation of the prevalence of Enterocytozoon bieneusi,Encephalitozoon intestinalis and Encephalitozoon cuniculi among diarrheal patients in the southwest of China

QIU Lu-yao^1,2, PING Jing^1,2, LI Wen-dao^1,2, DING Song-tao¹, LIU Han-deng¹

1. Center of Basic Medical Experiment, Chongqing Medical University, Chongqing 401331, China;
2. College of Pediatrics, Chongqing Medical University, Chongqing 401331, China

Received:2017-05-24 Online:2017-10-28 Published:2017-10-28

摘要/Abstract

摘要：

目的调查毕氏肠道微孢子虫（Enterocytozoon bieneusi）、肠炎微孢子虫（Encephalitozoon intestinalis）和兔脑炎微孢子虫（Encephalitozoon cuniculi）在我国西南地区的流行情况。方法收集了124份西南地区腹泻患者的粪便，通过显微镜镜检、PCR检测等手段，进行微孢子虫的分离与鉴定。结果毕氏肠道微孢子虫和肠炎微孢子虫的检出率较高，分别为7.26%（9/124）和3.23%（4/124），兔脑炎微孢子虫的检出率为0。结论西南地区腹泻患者有感染微孢子虫的情况，感染类型多为毕氏肠道微孢子虫和肠炎微孢子虫。

关键词: 微孢子虫, 腹泻患者, 感染, 人畜共患, PCR检测

Abstract:

Objective To investigate the prevalence of Enterocytozoon bieneusi,Encephalitozoon intestinalis and Encephalitozoon cuniculi among diarrheal patients in the southwest of China.Methods A total of 124 fecal specimens from patients with diarrhea syndrome were collected.The specimens were stained by modified trichrome (weber) and were examined under microscopy.The extracted DNA samples were evaluated by PCR amplification.Results The prevalence of E.bieneusi was 7.26% (9/124),and that of E.intestinalis was 3.23% (4/124),with E.cuniculi all negative.Conclusion The infection of microsporidia did exist in the southwest of China,and the most common types in this area were E.bieneusi and E.intestinalis.

Key words: microsporidia, diarrheal patients, infection, zoonotic, PCR amplification

中图分类号:

R519.8

邱璐瑶, 平静, 李文道, 丁嵩涛, 刘含登. 3种微孢子虫在我国西南部分地区腹泻患者中的流行情况调查[J]. 中国真菌学杂志, 2017, 12(5): 257-261.

QIU Lu-yao, PING Jing, LI Wen-dao, DING Song-tao, LIU Han-deng. Investigation of the prevalence of Enterocytozoon bieneusi,Encephalitozoon intestinalis and Encephalitozoon cuniculi among diarrheal patients in the southwest of China[J]. Chinese Journal of Mycology, 2017, 12(5): 257-261.

参考文献

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3种微孢子虫在我国西南部分地区腹泻患者中的流行情况调查

Investigation of the prevalence of Enterocytozoon bieneusi,Encephalitozoon intestinalis and Encephalitozoon cuniculi among diarrheal patients in the southwest of China

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