评价ITS、BenA和CaM序列分析在曲霉菌种鉴定方面的应用

中国真菌学杂志　 2017, Vol. 12 　Issue (2): 74-77.

评价ITS、BenA和CaM序列分析在曲霉菌种鉴定方面的应用

李颖, 徐英春

中国医学科学院北京协和医院检验科中国医学科学院北京协和医学院研究生院, 北京 100730

收稿日期:2016-11-22 出版日期:2017-04-28 发布日期:2017-04-28
通讯作者: 徐英春,E-mail:xycpumch@139.com E-mail:xycpumch@139.com
作者简介:李颖,女(汉族),博士研究生在读.E-mail:bravezane@163.com

Evaluation of ITS, BenA and CaM in identification of clinical Aspergillus

LI Ying, XU Ying-chun

Department of Clinical Laboratory, Peking Union Medical College Hospital, Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China

Received:2016-11-22 Online:2017-04-28 Published:2017-04-28

摘要/Abstract

摘要：

目的评价内转录间隔区（ITS）、β-微管蛋白基因（BenA）和钙调蛋白基因（CaM）序列分析技术对曲霉的鉴定能力。方法对169株曲霉临床分离株分别进行ITS、BenA和CaM序列测定，并在Genbank数据库中进行比对分析以获得其菌种鉴定信息。结果 169株曲霉经ITS、BenA和CaM序列分析分别有52.7%、66.3%、97.6%菌株鉴定至种水平，47.3%、33.7%、2.4%菌株鉴定至属水平，3个序列对曲霉均不存在无法鉴定情况。结论 ITS、BenA和CaM均可用于曲霉菌种鉴定，其中以CaM的鉴定能力最强。

关键词: 内转录间隔区 (ITS), β-微管蛋白基因 (BenA), 钙调蛋白基因 (CaM), 序列分析, 曲霉, 菌种鉴定

Abstract:

Objective To evaluate the internal transcribed spacer (ITS), β-tubulin gene (BenA) and calmodulin gene (CaM) sequence analysis in identification of clinical Aspergillus.Methods All 169 strains of Aspergillus collected from clinical patients were analyzed by ITS, BenA, CaM sequencing to obtain the species information with reference to the Genbank database.Results The tested strains were identified to species level with identification rates of 52.7% (89/169), 66.3% (112/169), 97.6% (165/169) and to genus level of 47.3% (80/169), 33.7% (57/169), 2.4% (4/169) by ITS, BenA and CaM, respectively.None of the three sequence markers failed in identification of Aspergillus.Conclusion ITS, BenA and CaM can be used to identify clinical Aspergillus.Among them, CaM is the most efficient molecular marker for Aspergillus identification.

Key words: ITS, BenA, CaM, sequence analysis, Aspergillus, identification

中图分类号:

R379.6

李颖, 徐英春. 评价ITS、BenA和CaM序列分析在曲霉菌种鉴定方面的应用[J]. 中国真菌学杂志, 2017, 12(2): 74-77.

LI Ying, XU Ying-chun. Evaluation of ITS, BenA and CaM in identification of clinical Aspergillus[J]. Chinese Journal of Mycology, 2017, 12(2): 74-77.

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