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中国真菌学杂志 2017, Vol. 12  Issue (1): 3-7.

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烟曲霉孢子对肥大细胞活化作用的研究

陈丽莎1, 李燕明2, 佟训靓3, 赵作涛4, 王辰1,5   

  1. 1. 北京大学中日友好临床医学院, 北京 100029;
    2. 北京医院呼吸与危重症医学科, 北京 100730;
    3. 北京医院老年医学部, 北京 100730;
    4. 北京大学第一医院皮肤性病科 北京大学真菌和真菌病研究中心, 北京 100034;
    5. 国家呼吸疾病临床研究中心 中日友好医院呼吸中心 中日友好医院呼吸与危重症医学科, 北京 100029
  • 收稿日期:2017-01-25 出版日期:2017-02-28 发布日期:2017-02-28
  • 通讯作者: 王辰,E-mail:cyh-birm@263.net E-mail:cyh-birm@263.net
  • 作者简介:陈丽莎,女(汉族),硕士研究生在读.E-mail:lychenls@qq.com
  • 基金资助:

    国家十二五科技支撑计划(2012BAI05B02)

Effects of Aspergillus fumigatus spore on the activation of mast cell

CHEN Li-Sha1, LI Yan-Ming2, TONG Xun-Liang3, ZHAO Zuo-Tao4, WANG Chen1,5   

  1. 1. Peking University China-Japan Friendship School of Clinical Medicine, Beijing 100029;
    2. Department of Respiratory and Critical Care Medicine, Beijing Hospital, Beijing 100730;
    3. Department of Geriatrics, Beijing Hospital, Beijing 100730;
    4. Departmemt of Dermatovenerology, Peking University First Hospital, Research Center for Medical Mycology, Beijing 100034;
    5. National Clinical Research Center for Respiratory Diseases, Center for Respiratory Diseases, China-Japan Friendship Hospital, Department of Respiratory and Critical Care Medicine, China-Japan Friendship Hospital, Beijing 100029
  • Received:2017-01-25 Online:2017-02-28 Published:2017-02-28

摘要:

目的 研究烟曲霉孢子对人肥大细胞系HMC-1的活化作用。方法 用加热灭活的方法制备烟曲霉孢子抗原,体外培养人肥大细胞系HMC-1细胞,检测热灭活烟曲霉孢子诱导HMC-1细胞脱颗粒β-氨基己糖苷酶释放比,酶联免疫吸附测定(ELISA)法检测HMC-1细胞培养上清中白细胞介素-5(IL-5)的释放,Realtime-PCR检测热灭活烟曲霉孢子诱导的HMC-1细胞IL-5 mRNA的表达变化。结果 与阴性对照组相比,热灭活烟曲霉孢子刺激后HMC-1细胞的β-氨基己糖苷酶释放比升高[(11.3%±1.2%)VS(0.5%±0.1%),P<0.05],IL-5释放增加[(11.96±1.09)VS(9.00±1.36),P<0.05],IL-5 mRNA表达上调[(2.47±0.12)VS(0.00±0.26),P< 0.05]。结论 热灭活烟曲霉孢子诱导HMC-1肥大细胞活化,引起细胞活化脱颗粒、合成和释放IL-5增加。

关键词: 烟曲霉, 孢子, 肥大细胞, 脱颗粒, 白细胞介素-5

Abstract:

Objective To study the effects of Aspergillus fumigatus spore on the activation of human mast cell line HMC-1.Methods Prepared heat-killed Aspergillus fumigatus spore antigen by heating method.HMC-1 cells were co-cultured with heat-killed Aspergillus fumigatus spores whose concentration was 5×105.Microplate spectrophotometer was applied to the β-hexosaminidase release assay which was an index of mast cell degranulation.Enzyme-linked immunosorbent assay (ELISA) was used for released IL-5.The expression levels of IL-5 mRNA was detected by Realtime-PCR.Results Compared with the negative control group,heat-killed Aspergillus fumigatus spores increased β-hexosaminidase release [(11.3%±1.2%) VS (0.5%±0.1%),P<0.05],IL-5 mRNA expression level [(2.47±0.12) VS (0±0.26),P<0.05] and the release of IL-5 [(11.96±1.09) VS (9±1.36),P<0.05] in HMC-1 cells.Conclusion Aspergillus fumigatus spores activates mast cell line HMC-1 resulting in cell degranulation,synthesis and release of IL-5.

Key words: Aspergillus fumigatus, spore, mast cell, degranulation, interleukin-5

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