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中国真菌学杂志 2016, Vol. 11  Issue (6): 378-380.

技术与方法 上一篇    下一篇

荧光染色法和KOH湿片法在诊断浅部真菌感染中的效果比较

陈锐君, 佟韬, 李超, 吴贤, 陈琼, 陈晓男, 徐明, 陈柏叡   

  1. 福州市皮肤病防治院, 福州 350001
  • 收稿日期:2016-08-03 出版日期:2016-12-28 发布日期:2016-12-28
  • 通讯作者: 徐明,E-mail:15359725945@163.com E-mail:15359725945@163.com
  • 作者简介:陈锐君,女(汉族),本科,初级检验技师.E-mail:mone882003@qq.com
  • 基金资助:

    福州市科技计划项目(2013-S-128-4)

Comparison of fluorescent staining method and KOH wet method for diagnosis of superficial fungal infections

CHEN Rui-jun, TONG Tao, LI Chao, WU Xian, CHEN Qiong, CHEN Xiao-nan, XU Ming, CHEN Bo-rui   

  1. Fuzhou Dermatosis Prevention Hospital, Fuzhou 350001
  • Received:2016-08-03 Online:2016-12-28 Published:2016-12-28

摘要:

目的 探讨荧光染色法在浅部真菌镜检中的应用价值。方法 收集100例皮肤科门诊疑似真菌感染的患者标本,分别运用荧光染色法和KOH湿片法对这些标本进行检测,并对检测结果进行比较。结果 荧光染色法共检出56例真菌阳性,而KOH湿片法只有39例阳性。荧光染色法的阳性检出率明显高于KOH湿片法(χ2=5.79,P<0.05)。结论 荧光染色法是一种快速、准确的真菌检测方法,值得在皮肤科对真菌感染早期诊断的推广使用。

关键词: 荧光染色法, KOH湿片法, 真菌感染

Abstract:

Objective To explore the application value of fluorescent staining method for superficial fungal microscopic examination.Methods One hundred cases with suspected fungal infection were collected in the department of dermatology.Fluorescent staining method and KOH wet method were used respectively to detect these samples,and the results were compared.Results A total of 56 cases of fungal positive were detected with fluorescent staining method,while there were only 39 cases of fungal positive with KOH wet method.The positive detection rate of fluorescent staining method was higher than that of KOH wet method (χ2=5.79,P<0.05).Conclusion The fluorescent staining method is a rapid and accurate method to detect fungi.It is worth to be used in the department of dermatology for the early diagnosis of fungal infection.

Key words: fluorescent staining method, KOH wet method, fungal infection

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