紫檀茋诱导白念珠菌凋亡的研究

中国真菌学杂志　 2016, Vol. 11 　Issue (2): 75-78.

紫檀茋诱导白念珠菌凋亡的研究

胡丹丹, 毕爽, 姜远英, 王彦

中国人民解放军第二军医大学药学院新药研究中心, 上海 200433

收稿日期:2015-10-08 出版日期:2016-04-28 发布日期:2016-04-28
通讯作者: 王彦,E-mail:wangyansmmu@126.com;姜远英,E-mail:jiangyuanying@126.com E-mail:wangyansmmu@126.com;jiangyuanying@126.com
作者简介:胡丹丹,女(汉族),博士研究生在读.E-mail:dandanhu1989@163.com;毕爽,女(汉族),硕士研究生在读.E-mail:shuangxun640@126.com
基金资助:
国家自然科学基金(81273558,81072678);上海市浦江人才计划(14PJD001)

Pterostilbene induces apoptosis in Candida albicans

HU Dan-dan, BI Shuang, JIANG Yuan-ying, WANG Yan

New Drug Research and Development Center, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

Received:2015-10-08 Online:2016-04-28 Published:2016-04-28

摘要/Abstract

摘要：

目的探究紫檀茋(Pterostilbene,PTE)诱导白念珠菌凋亡的活性。方法通过分析白念珠菌凋亡细胞、坏死细胞和活细胞比例,检测Caspase酶活性、细胞内活性氧和线粒体膜电位,评价PTE诱导白念珠菌凋亡的活性。结果 ≥4 μg/mL PTE处理后白念珠菌凋亡比例明显增加,Caspase酶活性显著升高,细胞内活性氧水平显著升高,线粒体膜电位显著降低。结论 PTE具有诱导白念珠菌凋亡的活性,该作用可能与活性氧积累和线粒体损伤相关。

关键词: 白念珠菌, 紫檀茋, 细胞凋亡

Abstract:

Objective To explore the activity of Pterostilbene (PTE) in inducing apoptosis of Candida albicans.Methods We used apoptosis, necrosis and overall viability assays, caspase activity determination, ROS measurement and mitochondrial membrane potential assay to evaluate the activity of PTE in inducing apoptosis of C.albicans.Results After C.albicans treated with ≥4 μg/ml PTE, the percentage of apoptosis cells and activity of Caspase notably increased.Moreover, the mitochondrial membrane potential decreased significantly and the intracellular ROS increased.Conclusions PTE has an apoptosis-inducing effect on C.albicans, and this effect may be associated with ROS accumulation and mitochondrial injure.

Key words: Candida albicans, pterostilbene, apoptosis

中图分类号:

R379.4

胡丹丹, 毕爽, 姜远英, 王彦. 紫檀茋诱导白念珠菌凋亡的研究[J]. 中国真菌学杂志, 2016, 11(2): 75-78.

HU Dan-dan, BI Shuang, JIANG Yuan-ying, WANG Yan. Pterostilbene induces apoptosis in Candida albicans[J]. Chinese Journal of Mycology, 2016, 11(2): 75-78.

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