多重连接探针扩增技术在临床病原体检测中的应用

中国真菌学杂志　 2015, Vol. 10 　Issue (5): 307-311.

多重连接探针扩增技术在临床病原体检测中的应用

方文捷, 刘加, 潘炜华, 廖万清

上海市医学真菌分子生物学重点实验室, 上海长征医院皮肤病与真菌病研究所全军皮肤病重点实验室, 第二军医大学附属长征医院皮肤科, 上海 200003

收稿日期:2014-10-26 出版日期:2015-10-28 发布日期:2015-10-28
通讯作者: 廖万清,E-mail:liaowanqing@sohu.com;潘炜华,E-mail:panweihua@medmail.com.cn E-mail:liaowanqing@sohu.com;panweihua@medmail.com.cn
作者简介:方文捷,男(汉族),硕士研究生在读.E-mail:fangwenjie1990@126.com
基金资助:
国家973项目(2013CB531601),重大传染病防治科技重大专项(2013ZX10004612),上海市科委专项(14DZ2272900)

MLPA application in clinical diagnosis of pathogen

FANG Wen-jie, LIU Jia, PAN Wei-hua, Liao Wanqing

Shanghai Key Laboratory of Molecular Medical Mycology, Institute of dermatology and fungal diseases, PLA Key laboratory of the fungal diseases, Department of dermatology, Changzheng hospital, Shanghai, 200433

Received:2014-10-26 Online:2015-10-28 Published:2015-10-28

摘要/Abstract

摘要：

真菌等临床病原体的诊断、基因分型和耐药性检测对于临床诊治十分重要。多重连接探针扩增是一个可以同时定量检测多个基因序列的,简单、有效、快速的方法。其步骤包括待测DNA变性、半探针与待测DNA杂交,半探针的连接、PCR法扩增、凝胶电泳或者毛细管电泳鉴定扩增产物。MLPA已经在临床微生物诊断和研究中有了一定的应用,虽然尚处于起步阶段,但因其操作简便,反应快速,高通量,高敏感性和特异性的特点,拥有良好的应用前景。

关键词: 多重连接探针扩增, 病原体, 诊断

Abstract:

As for patients diagnosed with Infectious diseases, favorable prognosis is determined by correct diagnosis and proper treatment.So it is important to diagnose clinical pathogens rapidly.Compared with other clinical test methods, MLPA is a simple, valid, and fast one which can detect several gene sequences in quantity at the same time.The steps include:the degeneration of DNA sample, hybridization, ligation, and PCR amplification.Although being at its first stage, there are already certain applications of MLPA in diagnosing and researching of clinic microorganism, and the peculiarity of easy operation, fast reaction, high pass rate and high sensitivity have reveal bright prospect of MLPA.

Key words: multiplex ligation-dependent probe amplification, pathogen, diagnosis

中图分类号:

R446.5

方文捷, 刘加, 潘炜华, 廖万清. 多重连接探针扩增技术在临床病原体检测中的应用[J]. 中国真菌学杂志, 2015, 10(5): 307-311.

FANG Wen-jie, LIU Jia, PAN Wei-hua, Liao Wanqing. MLPA application in clinical diagnosis of pathogen[J]. Chinese Journal of Mycology, 2015, 10(5): 307-311.

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