氟曲马唑对足癣分离病原菌的体外抗菌活性研究

中国真菌学杂志　 2015, Vol. 10 　Issue (5): 272-275.

氟曲马唑对足癣分离病原菌的体外抗菌活性研究

李亚丽, 万喆, 陈伟, 王爱平, 李若瑜

北京大学第一医院皮肤性病科, 北京大学真菌与真菌病研究中心, 皮肤病分子诊断北京市重点实验室, 北京 100034

收稿日期:2015-03-18 出版日期:2015-10-28 发布日期:2015-10-28
通讯作者: 王爱平,E-mail:wangap@medmail.com.cn E-mail:wangap@medmail.com.cn
作者简介:李亚丽,女(汉族),博士研究生在读.E-mail:lyl2007happy@126.com

In vitro activities of flutrimazole against fungal pathogens isolated from tinea pedis

LI Ya-li, WAN Zhe, CHEN Wei, WANG Ai-ping, LI Ruo-yu

Department of Dermatology and Venereology, Peking University First Hospital, Research Center for Medical Mycology, Peking University, and Beijing Key Laboratory of Molecular Diagnosis of Dermatoses, Beijing 100034, China

Received:2015-03-18 Online:2015-10-28 Published:2015-10-28

摘要/Abstract

摘要：

目的评价氟曲马唑对足癣分离病原菌的体外抗菌活性,并与联苯苄唑相比。方法采用CLSI推荐的M-38 A2(皮肤癣菌)和M27-A3(酵母菌)微量液基稀释法对病原菌进行体外药物敏感性测定。结果氟曲马唑对红色毛癣菌最小抑菌浓度(MIC)范围为0.031~2μg/mL,MIC₅₀为0.5μg/mL,MIC₉₀为1μg/mL,GM值为0.637μg/mL;联苯苄唑分别为0.031~16μg/mL,0.25μg/mL,2μg/mL,0.634μg/mL;两药对红色毛癣菌MIC GM值比较差异无统计学意义(P=0.974)。氟曲马唑对趾(指)间毛癣菌MIC范围为0.031~1μg/mL,MIC₅₀为0.031μg/mL,MIC₉₀为0.5μg/mL,GM值为0.17μg/mL;联苯苄唑分别为0.125~16μg/mL,1μg/mL,2μg/mL,1.886μg/mL;两药对趾(指)间毛癣菌MIC GM值比较差异具有统计学意义(P=0.001)。尽管酵母菌菌株数偏少,但研究结果显示两药对念珠菌属和毛孢子菌属MIC GM值比较差异具有统计学意义(P=0.000和P=0.031)。结论氟曲马唑对红色毛癣菌的抗菌活性与联苯苄唑相似,但对趾(指)间毛癣菌、念珠菌属和毛孢子菌属的抗菌活性明显优于联苯苄唑。

关键词: 氟曲马唑, 联苯苄唑, 皮肤癣菌, 酵母菌, 体外抗菌活性

Abstract:

Objective To evaluate the in vitro activities of flutrimazole against fungal pathogens isolated from tinea pedis.Methods In vitro drug sensitivities were determined using the broth microdilution methods according to the CLSI M38-A2(for dermatophytes) and M27-A3(for yeasts) documents.Results When against Trichophyton rubrum, the range of minimum inhibitory concentration(MIC), MIC₅₀, MIC₉₀, and geometric mean(GM) MIC for flutrimazole were 0.031~2μg/mL, 0.5μg/mL, 1μg/mL and 0.637μg/mL;for bifonazole were 0.031~16μg/mL, 0.25μg/mL, 2μg/mL and 0.634μg/mL;no significant(P=0.974) difference was found for the GM MIC between the two drugs.When against Trichophyton interdigitale, the range of MIC, MIC₅₀, MIC₉₀, and GM MIC for flutrimazole were 0.031~1μg/mL, 0.031μg/mL, 0.5μg/mL and 0.17μg/mL;for bifonazole were 0.125~16μg/mL, 1μg/mL, 2μg/mL and 1.886μg/mL;significant(P=0.001) difference was found for the GM MIC between the two drugs.Even though there are a few number of yeast strains, this study showed that the two drugs had significant difference for the GM MIC against Candida spp.(P=0.000) and Trichosporon spp.(P=0.031).Conclusion In vitro activity of flutrimazole was similar to bifonazole when against T.rubrum, but better than bifonazole when against T.interdigitale, Candida spp.and Trichosporon spp..

Key words: flutrimazole, bifonazole, dermatophytes, yeasts, in vitro

中图分类号:

R978.5

李亚丽, 万喆, 陈伟, 王爱平, 李若瑜. 氟曲马唑对足癣分离病原菌的体外抗菌活性研究[J]. 中国真菌学杂志, 2015, 10(5): 272-275.

LI Ya-li, WAN Zhe, CHEN Wei, WANG Ai-ping, LI Ruo-yu. In vitro activities of flutrimazole against fungal pathogens isolated from tinea pedis[J]. Chinese Journal of Mycology, 2015, 10(5): 272-275.

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