不同光敏剂孵育时间在白念珠菌光动力抑制效应中的实验研究

中国真菌学杂志　 2015, Vol. 10 　Issue (3): 146-150.

不同光敏剂孵育时间在白念珠菌光动力抑制效应中的实验研究

徐辉¹, 陈虹霞², 邹先彪²

1. 解放军医学院, 北京 100853;
2. 解放军总医院第一附属医院皮肤科, 北京 100048

收稿日期:2014-12-18 出版日期:2015-06-28 发布日期:2015-06-28
通讯作者: 邹先彪, E-mail:xbzou@126.com E-mail:xbzou@126.com
作者简介:徐辉, 男 (汉族), 硕士研究生在读, 主治医师.E-mail:81764988@qq.com

Inhibitory effect of different durations of incubation of a photosensitizer on photodynamics of C.albicans

Xu Hui¹, CHENG Hong-xia², ZOU Xian-biao²

1. Chinese PLA Medical School, Beijing 100853, China;
2. Department of Dernatlogy, First Affiliated Hospital of Chinese PLA General Hospital, Beijng 100048, China

Received:2014-12-18 Online:2015-06-28 Published:2015-06-28

摘要/Abstract

摘要：

目的通过测定5-氨基酮戊酸 (5-ALA)与白念珠菌菌悬液不同孵育时间生成PpIX的水平及5-氨基酮戊酸光动力疗法 (5-ALA-PDT)抑菌率,为临床选择最佳孵育时间提供理论依据。方法制备白念珠菌悬液并与ALA避光孵育,设实验组和对照组。采用激光共聚焦显微镜观察PpIX产生情况,MTT法测定ALA光动力治疗对白念珠菌生长的抑制率。结果白念珠菌菌悬液与ALA避光孵育后有荧光物质PpIX产生,孵育30 min到90 min产生的PpIX最多,120 min以后PpIX开始明显减少,对照组无荧光物质出现。结论 ALA-PDT对白念珠菌抑制效应同孵育时间密切相关。这为临床ALA-PDT治疗白念珠菌疾病提供了理论依据。

关键词: 5-氨基酮戊酸, 光动力, 白念珠菌, 孵育时间

Abstract:

Objective To facilitate the selection of the best lighting time by determining the level at which C.albicans and 5-ALA PDT generate protoporphyrin IX (PpIX) at different time points and by measuring the bacteriostatic rate of 5-amino ketones pentanoic acid photodynamic therapy (5-ALA PDT).Method C.albicans suspension was prepared and incubated with ALA in the dark.The consequent mixture was divided into the control group and experimental group.The generation of PpIX was observed under a laser confocal microscope.The inhibitory rate of 5-ALA PDT on C.albicans was determined by MTT method.Results After dark incubation,C.albicans and ALA produced fluorescent PpIX that reached the peak amount between 30 min and 90 min,but began to decrease significantly at 120 min.There was no fluorescent material in control group.Conclusion The inhibitory effect of ALA PDT on C.albicans was closely related to the concentration of the photosensitizer,which provides data for treatment of C.albicans disease.

Key words: 5-amino ketones pentanoic acid, photodynamic, C.albicans, drug concentration

中图分类号:

R379.4

徐辉, 陈虹霞, 邹先彪. 不同光敏剂孵育时间在白念珠菌光动力抑制效应中的实验研究[J]. 中国真菌学杂志, 2015, 10(3): 146-150.

Xu Hui, CHENG Hong-xia, ZOU Xian-biao. Inhibitory effect of different durations of incubation of a photosensitizer on photodynamics of C.albicans[J]. Chinese Journal of Mycology, 2015, 10(3): 146-150.

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