顾菊林无绿藻分子生物学鉴定与分型研究新进展

中国真菌学杂志　 2015, Vol. 10 　Issue (2): 126-128.

综述上一篇

顾菊林无绿藻分子生物学鉴定与分型研究新进展

李文静^1,2, 刘锦燕², 史册^1,2, 王影^1,2, 孙景勇³, 项明洁^1,2

1. 上海交通大学医学院附属瑞金医院检验科, 上海 200025;
2. 上海交通大学医学院附属瑞金医院卢湾分院放免检验科, 上海 200020;
3. 上海交通大学医学院附属瑞金医院临床微生物科, 上海 200025

收稿日期:2014-11-28 出版日期:2015-04-28 发布日期:2015-04-28
通讯作者: 项明洁,E-mail:mjxiang123456@126.com E-mail:mjxiang123456@126.com
作者简介:李文静,女(汉族),硕士研究生在读.E-mail:15000028775@163.com

New advance in the study of the molecular biological identification and typing of Prototheca

LI Wen-jing^1,2, LIU Jin-yan², SHI Ce^1,2, WANG Ying^1,2, SUN Jing-yong³, XIANG Ming-jie^1,2

1. Department of Laboratory, Ruijn Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025;
2. Radioimmunology and Clinical Laboratory, Luwan Branch, Ruijn Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200020;
3. Department of Clinical Microbiology Laboratory, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025

Received:2014-11-28 Online:2015-04-28 Published:2015-04-28

摘要/Abstract

摘要：

以基因序列为鉴定基础的分子生物学方法以其客观、可重复性好等优点,被认为是分子鉴定无绿藻的"金标准"。该文对近年来应用在无绿藻鉴定和分型方面的实时定量PCR (Real-Time Quantitative PCR,RT-PCR)技术、单链构象多态性 (Single Stranded Conformation Polymophism,SSCP)分析、限制性片段长度多态性 (Restriction Fragment Length Polymorphism,RFLP)分析、高分辨率熔解曲线 (High Resolution Melting,HRM)分析、质谱 (Mass Spectrometry,MS)分析等分子生物学新技术和新方法进行综述。

关键词: 无绿藻, 分子生物学, PCR, 质谱

Abstract:

The molecular biology method based on gene sequencing which is objective,highly reproducible and so on,is considered the "gold standard" of Prototheca's molecular identification.To summarize the new molecular biology methods and techniques applied to Prototheca's identification and typing,such as real-time quantitative PCR (RT-PCR) technology,single strand conformation polymorphism (SSCP) analysis,PCR-restriction fragment length polymorphism (RFLP)analysis,high resolution melting (HRM) analysis,mass spectrometry(MS) analysis.

Key words: Prototheca, Molecular biology, PCR, MS

中图分类号:

R519.5

李文静, 刘锦燕, 史册, 王影, 孙景勇, 项明洁. 顾菊林无绿藻分子生物学鉴定与分型研究新进展[J]. 中国真菌学杂志, 2015, 10(2): 126-128.

LI Wen-jing, LIU Jin-yan, SHI Ce, WANG Ying, SUN Jing-yong, XIANG Ming-jie. New advance in the study of the molecular biological identification and typing of Prototheca[J]. Chinese Journal of Mycology, 2015, 10(2): 126-128.

参考文献

[1] Cremonesi P,Pozzi F,Ricchi M,et al.Technical note:Identification of Prototheca species from bovine milk samples by PCR-single strand conformation polymorphism
[J].J Dairy Sci,2012,95(12):6963-6968.

[2] Roesler U,Möller A,Hensel A,et al.Diversity within the current algal species Prototheca zopfii:A proposal for two Prototheca zopfii genotypes and description of a novel species,Prototheca blaschkeae sp.nov.
[J].Int J Syst Evol Microbiol,2006,56(pt6):1419-1425.

[3] 章强强.中国无绿藻病发病的现状分析及其诊治
[J].实用皮肤病学杂志,2013,6(2):65-67.

[4] Ueno R,Hanagata N,Urano N,et al.Molecular phylogeny and phenotypic variation in the heterotrophic green algal genus Prototheca
[J].J Phycol,2005,41(6):1268-1280.

[5] Stoh K,Ooe K,Nagayama H,et a1.Prototheca cutis sp.nov.,a newly discovered pathogen of protothecosis isolated from inflamed human skin
[J].Int J Syst Evol Microbiol,2010,60(Pt 5):1236-1240.

[6] Ricchi M,Goretti M,Branda E,et al.Molecular characterization of Prototheca strains isolated from Italian dairy herds
[J].J Dairy Sci,2010,93(10):4625-4631.

[7] Todd JR,King JW,Oberle A,et al.Protothecosis:report of a case with 20-year follow-up,and review of previously published cases
[J].Med Mycol,2012,50 (7):673-689.

[8] Möller A,Truyen U,Roesler U.Prototheca zopfii genotype 2:The causative agent of bovine protothecal mastitis?
[J].Vet Microbiol,2007,120(3-4):370-374.

[9] Osumi T,Kishimoto Y,Kano R,et al.Prototheca zopfii genotypes isolated from cow barns and bovine mastitis in Japan
[J].Vet Microbiol,2008,131(3-4):419-423.

[10] Hirose N,Nishimura K,Inoue-Sakamoto M,et al.Ribosomal Internal Transcribed Spacer of Prototheca wickerhamii Has Characteristic Structure Useful for Identification and Genotyping
[J].PLoS One,2013,8(11):e81223.

[11] Sobukawa H,Yamaguchi S,Kano R,et al.Short communication:Molecular typing of Prototheca zopfii from bovine mastitis in Japan
[J].J Dairy Sci,2012,95(8):4442-4446.

[12] Onozaki M,Makimura K,Satoh K,et al.Detection and Identification of Genotypes of Prototheca zopfii in Clinical Samples by Quantitative PCR Analysis
[J].Infect Dis,2013,66(5):383-390.

[13] Cremonesi P,Pozzi F,Ricchi M,et al.Technical note:Identification of Prototheca species from bovine milk samples by PCR-single strand conformation polymorphism
[J].J Dairy Sci,2012,95(12):6963-6968.

[14] 赵鎏莉,王晓萍.细菌鉴定的分子生物学方法
[J].哈尔滨师范大学自然科学学报,2012,28(4):53-56.

[15] Jagielski T,Lassa H,Ahrholdt J,et al.Genotyping of bovine Prototheca mastitis isolates from Poland
[J].Vet Microbiol,2011,149(1-2):283-287.

[16] 于静波.PCR-HRM技术在微生物鉴定中的应用
[J].国际检验医学杂志,2011,32(17):1988-1989.

[17] Sobukawa H,Ibaraki M,Kano R,et al.Rapid Molecular Typing of Prototheca zopfii by High Resolution Melting Real-Time PCR(PCR-HRM)
[J].Med Mycol J,2013 54(4):341-344.

[18] Wang X,Fu Y-F,Wang R-Y,et al.Identification of Clinically Relevant Fungi and Prototheca Species by rRNA Gene Sequencing and Multilocus PCR Coupled with Electrospray Ionization Mass Spectrometry
[J].PLOS ONE,2014,9(5):e98110.

[19] 张丽,徐英春.MALDI-TOF MS在临床微生物实验室的应用研究进展
[J].中国感染与化疗杂志,2013,13(3):226-231.

[20] Von Bergen M,Eidner A,Schmidt F,et al.Identification of harmless and pathogenic algae of the genus Prototheca by MALDI-MS
[J].Proteomics Clin Appl,2009,3(7):774-784.

[21] Murugaiyan J,Ahrholdt J,Kowbel V,et al.Establishment of a matrix-assisted laser desorption ionization time-offlight mass spectrometry database for rapid identification of infectious achlorophyllous green micro-algae of the genus Prototheca
[J].Clin Microbiol Infect,2012,18(5):461-467.

[22] 章强强,赵颖.无绿藻的表型及其分子生物学鉴定
[J].中国真菌学杂志,2010,5(1):9-12.

顾菊林无绿藻分子生物学鉴定与分型研究新进展

New advance in the study of the molecular biological identification and typing of Prototheca

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	刘加, 梁官钊, 刘维达. 絮状表皮癣菌的基础与临床研究进展[J]. 中国真菌学杂志, 2019, 14(6): 376-380.
[2]	张明瑞, 周盈, 李福秋, 姚春丽, 杨鑫, 龚杰, 赵飞. 实时荧光PCR检测巴西孢子丝菌的实验方法的建立[J]. 中国真菌学杂志, 2019, 14(5): 297-302.
[3]	余甜, 林赋桂, 胡立冬, 李可可, 陈晓青, 谢跃, 时文静, 魏莲花. 飞行质谱鉴定临床分离真菌准确度的Meta分析[J]. 中国真菌学杂志, 2019, 14(2): 83-89.
[4]	邵锦, 余进, 万喆, 李若瑜, 王爱平. 肺曲霉球1例及实验研究[J]. 中国真菌学杂志, 2019, 14(1): 24-27.
[5]	罗柳春, 黄春兰, 梁辉苍, 徐秋红, 韦文飞, 覃仁利. 小型无绿藻所致皮肤感染1例[J]. 中国真菌学杂志, 2018, 13(6): 361-362.
[6]	郑艳青, 史娜娜, 曹存巍. 实时荧光定量PCR检测马尔尼菲篮状菌病组织样本中菌载量的研究[J]. 中国真菌学杂志, 2018, 13(2): 71-74.
[7]	杨金, 章强强. 无绿藻的微生物学特性和菌种鉴定[J]. 中国真菌学杂志, 2018, 13(2): 95-100.
[8]	赵悦, 朱巍巍, 刘锦燕, 孟玲宁, 王珏婷, 李文静, 项明洁. 无绿藻PCR ITS1-ITS2基因分型法的建立及应用[J]. 中国真菌学杂志, 2018, 13(1): 4-7.
[9]	邱璐瑶, 平静, 李文道, 丁嵩涛, 刘含登. 3种微孢子虫在我国西南部分地区腹泻患者中的流行情况调查[J]. 中国真菌学杂志, 2017, 12(5): 257-261.
[10]	高来强, 王海英, 王学红, 李爱兰, 马强, 张宏. 白念珠菌氟康唑耐药株与敏感株SRB1、CDR1、ERG11表达比较分析[J]. 中国真菌学杂志, 2017, 12(3): 148-151,155.
[11]	尤丽吐孜·许合热提, 哈地丽亚·哈斯木, 王晓东, 邓淑文, 帕丽达·阿布利孜. 新疆首次临床分离新生隐球菌菌株分子鉴定、基因分型及体外药物敏感性研究[J]. 中国真菌学杂志, 2016, 11(6): 327-331.
[12]	曾炫皓, 章强强. 全球近10年无绿藻病例报道文献的回顾[J]. 中国真菌学杂志, 2016, 11(5): 310-315.
[13]	王立朋, 鲍翠霞, 于丽梅, 张晓录, 于威娟, 张霞, 李玮, 黄葆华, 李杰, 孙成铭. 核酸序列依赖性扩增、Real-time PCR及GM试验诊断侵袭性曲霉菌感染的临床应用评价[J]. 中国真菌学杂志, 2015, 10(5): 283-287.
[14]	张德全, 刘涵, 廖勇, 夏志宽, 杨冬倩, 杨阳, 吕雪莲, 杨蓉娅. 菌落PCR改良技术快速鉴定阿萨希毛孢子菌的实验研究[J]. 中国真菌学杂志, 2015, 10(1): 6-10.
[15]	李妍淳, 胡志东. 分子生物学技术在侵袭性真菌感染诊疗中的应用进展[J]. 中国真菌学杂志, 2014, 9(6): 377-380,376.