白头翁汤正丁醇提取物对白念珠菌VVC临床株黏附作用的影响

中国真菌学杂志　 2015, Vol. 10 　Issue (1): 25-30.

白头翁汤正丁醇提取物对白念珠菌VVC临床株黏附作用的影响

张梦翔¹, 夏丹¹, 施高翔¹, 陆克乔¹, 邵菁¹, 吴大强¹, 汪天明², 汪长中¹

1. 安徽中医药大学中西医结合临床学院, 合肥 230038;
2. 安徽省中医药科学院中西医结合研究所, 合肥, 230038

收稿日期:2014-11-21 出版日期:2015-02-28 发布日期:2015-02-28
通讯作者: 汪长中,E-mail:ahwcz63@sina.com E-mail:ahwcz63@sina.com
作者简介:张梦翔,男(汉族),硕士研究生在读.E-mail:tojosaki@126.com
基金资助:
安徽省自然科学基金(1408085MH165),安徽中医药大学校级科研项目(2014zr026)

Anti-adhesion effect of Butyl alcohol extract of BaiTouWeng decoction on Candida albicans isolated from VVC

ZHANG Meng-xiang¹, XIA Dan¹, SHI Gao-xiang¹, LU Ke-qiao¹, SHAO Jing¹, WU Da-qiang¹, WANG Tian-ming², WANG Chang-zhong¹

1. School of Integrated Traditional and Western Medicine, Anhui University of Chinese Medicine, Hefei 230038, China;
2. Institute of Integrated Traditional and Western Medicine, Anhui Academy of Chinese Medicine, Hefei 230038, China

Received:2014-11-21 Online:2015-02-28 Published:2015-02-28

摘要/Abstract

摘要： 目的探讨白头翁汤正丁醇提取物 (Butyl alcohol extract of BaiTouWeng decoction,BAEB)对外阴阴道念珠菌病(Vulvovaginal Candidiasis,VVC)白念珠菌临床分离株黏附作用的影响.方法平板法检测与BAEB共培养2 h、4 h时白念珠菌的CFU;XTT还原法检测与BAEB共培养2 h、4 h的白念珠菌代谢活性;试管法检测白念珠菌絮凝能力;水-烃两相法检测白念珠菌细胞表面疏水性 (cell surface hydrophobicity,CSH);荧光显微镜观察菌体活力;扫描电镜观察白念珠菌细胞形态;qRT-PCR法检测黏附相关基因ALS1、CSH1、HWP1、ALS5、ALA1、INT1、MNT2与ALS3的表达.结果 512、1 024 μg/mL BAEB可显著抑制黏附2 h、4 h时的白念珠菌的CFU及代谢活性;肉眼和倒置显微镜下观察发现512、1 024 μg/mL BAEB可明显延缓絮凝产生的时间;256、512、1 024 μg/mL的BAEB均能够显著减少白念珠菌的细胞表面疏水性;qRT-PCR检测表明1 024 μg/mL的BAEB作用后,ALS1、CSH1、ALA1、ALS3、INT1、HWP1分别下调18.7%、36%、37.8%、29.6%、19.9%、21.6%,ALS5与MNT2未产生明显变化.结论 BAEB可能通过调节黏附相关基因抑制白念珠菌VVC临床株的黏附作用.

关键词: 白头翁汤正丁醇提取物, 外阴阴道念珠菌病, 白念珠菌, 黏附

Abstract: Objective To investigate anti-adhesion effect of Butyl alcohol extract of BaiTouWeng decoction (BAEB)on Candida albicans.Methods Agar plate assay was used to determine CFU of Candida albicans for 2h and 4h.XTT reduction assay was applied to evaluate the adherent Candida albicans merabolic activity.Test tube assay was utilized to evaluate flocculation.The water-hydrocarbon two-phase assay was employed to measure the cell surface hydrophobicity of Candida albicans.Scanning electron microscope(SEM)and fluorescein diacetate(FDA)staining was used to observe the morphological changes of adherent Candida albicans.qRT-PCR was adopted to inspect the expression of adhesion-related genes such as INT1,ALS3,MNT2,ALA1,ALS1,ALS5,HWP1 and CSH1.Results 512,1 024 μg/mL BAEB could effectively inhibit adherence of Candida albicans.Flocculation was delayed by 512,1 024 μg/mL BAEB.The cell surface hydrophobicity in the BAEB treated groups was lower than that of the control group.The expression of ALS1,CSH1,ALA1,ALS3,INT1,HWP1 were downregulated dramatically after BAEB treatment,but ALS5 and MNT2 were unchanged.Conclusion BAEB could effectively inhibit adherence of Candida albicans isolated from VVC by regulating adhesion-associated genes.

Key words: butyl alcohol extract of BaiTouWeng decoction (BAEB), vulvovaginal candidiasis(VVC), Candida albicans, adhesion

中图分类号:

R379.4

张梦翔, 夏丹, 施高翔, 陆克乔, 邵菁, 吴大强, 汪天明, 汪长中. 白头翁汤正丁醇提取物对白念珠菌VVC临床株黏附作用的影响[J]. 中国真菌学杂志, 2015, 10(1): 25-30.

ZHANG Meng-xiang, XIA Dan, SHI Gao-xiang, LU Ke-qiao, SHAO Jing, WU Da-qiang, WANG Tian-ming, WANG Chang-zhong. Anti-adhesion effect of Butyl alcohol extract of BaiTouWeng decoction on Candida albicans isolated from VVC[J]. Chinese Journal of Mycology, 2015, 10(1): 25-30.

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