白念珠菌破壁方法的研究应用进展

中国真菌学杂志　 2014, Vol. 9 　Issue (5): 310-315.

白念珠菌破壁方法的研究应用进展

王晓飞¹, 张璐璐², 曹永兵^1,2, 姜远英^1,2

1. 福建中医药大学药学院中药学教研室, 福州 350108;
2. 中国人民解放军第二军医大学药学院新药研究中心, 上海 200433

收稿日期:2013-03-18 出版日期:2014-10-28 发布日期:2014-10-28
通讯作者: 姜远英, E-mail:jiangyycn@yahoo.com.cn E-mail:jiangyycn@yahoo.com.cn
作者简介:王晓飞, 男 (汉族), 硕士研究生在读.E-mail:wangxiaofei3@126.com
基金资助:
国家自然科学基金(81173635,81273556)

The research progress of Candida albicans wall-breaking method

WANG Xiao-fei¹, ZHANG Lu-lu², CAO Yong-bing^1,2, JIANG Yuan-ying^1,2

1. Department of Traditional Chinese materia medica, School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China;
2. R&D Center of New Drug, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

Received:2013-03-18 Online:2014-10-28 Published:2014-10-28

摘要/Abstract

摘要： 细胞壁作为真菌中特殊和必须的细胞结构,相对于哺乳动物的细胞膜更加坚硬,难以用简单的方法使其充分破碎.因此,达到理想的破壁效果是白念珠菌研究中的关键步骤之一.在提取白念珠菌RNA、DNA和蛋白质等细胞组分的过程中,为获得足量和稳定的实验样品.该文对多种白念珠菌破壁方法作一综述,以便为白念珠菌相关研究提供适用、高效的破壁方案.

关键词: 白念珠菌, 细胞壁, 破碎

Abstract: Cell wall as a special and necessary cell structure of fungus.it's hard to make it fully broken in a simple way. Therefore, to achieve the ideal wall-breaking effect is one of the key steps in the study of Candida albicans. In extraction such as RNA, DNA and protein components of Candida albicans, in order to obtain sufficient and stable experimental samples, the researchers shoulduse different methods breaking the cell wall according to different research purposes. This paper summarizes a variety of Candida albicans wall-breaking methods, so as to provide applicable and efficient solution of wall-breaking for related research in Candida albicans.

Key words: Candida albicans, cell wall, breaking

中图分类号:

R379.4

王晓飞, 张璐璐, 曹永兵, 姜远英. 白念珠菌破壁方法的研究应用进展[J]. 中国真菌学杂志, 2014, 9(5): 310-315.

WANG Xiao-fei, ZHANG Lu-lu, CAO Yong-bing, JIANG Yuan-ying. The research progress of Candida albicans wall-breaking method[J]. Chinese Journal of Mycology, 2014, 9(5): 310-315.

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